Recombinant Mouse Cadherin-6/KCAD Protein, CF Summary
Product Specifications
Optimal dilutions should be determined by each laboratory for each application.
Met1-Ala615 with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6826-CA
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in DPBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Cadherin-6/KCAD
Cadherin‑6, also known as KCAD or K‑Cadherin, is a 110‑120 kDa type I transmembrane glycoprotein belonging to the classical Cadherin superfamily of
calcium‑dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1, 2). Mouse Cadherin‑6 is synthesized with a 18 amino acid (aa) signal peptide and a 35 aa N‑terminal propeptide. The mature cell surface‑expressed protein consists of a 562 amino acid (aa) extracellular domain (ECD) that contains five tandem Cadherin repeats, a 21 aa transmembrane segment, and a 154 aa cytoplasmic domain (3). Within the ECD, mouse Cadherin‑6 shares 96% aa sequence identity with human and rat Cadherin‑10. It interacts homotypically as well as heterotypically with Cadherin‑9 and more weakly with Cadherins‑7, ‑10, and ‑14 (4, 5). Cadherin‑6 is expressed primarily in the kidney and brain, both during development and in the adult (6‑9). It is enriched in renal proximal tubule epithelial cells and their precursors at sites of cell‑cell contact as well as on basolateral membranes (7, 10, 11). In the nervous system, Cadherin‑6 is neuronally expressed in discrete regions of the neural plate, neural crest cells that give rise to peripheral nerves, and the brain (3, 6). Cadherin‑6 is additionally expressed in the developing retina, osteoclasts and their progenitors, ovarian epithelium, and endometrium during the follicular phase of the menstrual cycle (12‑15). It is also expressed in carcinomas of the kidney, ovary, and liver (8, 10, 11, 14).
- Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
- Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
- Inoue, T. et al. (1997) Dev. Biol. 183:183.
- Shimoyama, Y. et al. (2000) Biochem. J. 349:159.
- Shimoyama, Y. et al. (1999) J. Biol. Chem. 274:11987.
- Inoue, T. et al. (1998) Dev. Dyn. 211:338.
- Xiang Y.-Y. et al. (1994) Cancer Res. 54:3034.
- Shimoyama, Y. et al. (1995) Cancer Res. 55:2206.
- Cho, E.A. et al. (1998) Development 125:803.
- Paul, R. et al. (1997) Cancer Res. 57:2741.
- Shimazui, T. et al. (2000) Eur. Urol. 38:331.
- Ruan, G. et al. (2006) Mech. Dev. 123:881.
- Mbalaviele, G. et al. (1998) J. Cell Biol. 141:1467.
- Sellar, G.C. et al. (2001) Cancer Res. 61:6977.
- Getsios, S. et al. (1998) Dev. Dyn. 211:238.
Citation for Recombinant Mouse Cadherin-6/KCAD Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Raludotatug Deruxtecan, a CDH6-Targeting Antibody-Drug Conjugate with a DNA Topoisomerase I Inhibitor DXd, is Efficacious in Human Ovarian and Kidney Cancer Models
Authors: Suzuki, H;Nagase, S;Saito, C;Takatsuka, A;Nagata, M;Honda, K;Kaneda, Y;Nishiya, Y;Honda, T;Ishizaka, T;Nakamura, K;Nakada, T;Abe, Y;Agatsuma, T;
Molecular cancer therapeutics
Species: N/A
Sample Types: Recombinant Protein
Applications: ELISA Capture
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