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Recombinant Mouse BTNL3 Fc Chimera Protein, CF

Catalog #: 10222-BT Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
10222-BT-050
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse BTNL3 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.4-4 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse BTNL3 protein
Recombinant Mouse BTNL3
(Glu28-Pro241)
Accession # Q7TST0.3		IEGRMDPMouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
Q7TST0.3
N-terminal Sequence
Analysis
Glu28
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
51 kDa
SDS-PAGE
45-65 kDa, under reducing conditions

Product Datasheets

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10222-BT

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10222-BT

Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Bioactivity View Larger

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.4-4 μg/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse BTNL3 Fc Chimera (Catalog # 10222-BT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-65 kDa and 90-130 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BTNL3

Butyrophilin-like 3 (BTNL3), also referred to as BTNL1, Gm316, and Ng10, is a member of the BTN/MOG Ig-superfamily and shares structural resemblance to the B7 family (1). BTNL3 is a type I transmembrane protein that consists of an extracellular domain (ECD) containing two IgV domains, a single transmembrane domain, and a cytoplasmic domain with a B30.2/SPRY region. Mature mouse BTNL3 shares 30% and 80% amino acid sequence identity with human and rat BTNL3, respectively. BTNL3 mRNA has been identified in many tissues including small intestine, colon, liver, lung, bone marrow and spleen tissue, and it is highly expressed in neutrophils (2-6). The specific function of BTNL3 has yet to be fully elucidated, but BTNL3 is suggested to be a novel negative regulator for T cell activation and immune diseases (7). In humans, BTNL3 has been shown to form heterodimers with BTNL8 and overexpression of this complex leads to CD69 upregulation and T cell antigen receptor downregulation (8). In mice, BTNL3 can form heterodimers with BTNL6 and modulate local immune responses and intraepithelial lymphocytes–epithelial cell interaction pathways (9). Additionally, BTNL3 has been shown to bind directly to V gamma 4+ T cell receptor, suggesting a role in gamma δ T cell regulation (10). BTNL3 expression has also been found to be down-regulated in colon cancer tumors (11). R&D Systems in house data indicate that mouse BTNL3 protein inhibits the secretion of IL-2 from anti-CD3 activated mouse CD3+ T cells.

References
  1. Shibui, A. et al. (1999) J. Hum. Genet. 44:249.
  2. Arnett, H.A. et al. (2014) Nat Rev Immunol. 14:5596.
  3. Arnett, H.A. et al. (2009) Cytokine 46:370.
  4. Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
  5. Rhodes, D. et al. (2016) Annu. Rev. Immunol. 34:151.
  6. Guo, Y and Wang, AY (2015) Front. Immunol. 6:421.
  7. Yamazaki, T. et al. (2010) J Immunol 185:5907.
  8. Melandri, D. et al. (2018) Nat Immunol 19:1352.
  9. Lebrero-Fernández, C. et al. (2016) Front Immunol. 7:1.
  10. Wilcox, C.R. et al. (2019) Immunity 51:813.
  11. Lebrero-Fernandez, C. et al. (2016) Immun. Inflammation Dis. 4:191.
Long Name
Butyrophilin-like 3
Entrez Gene IDs
10917 (Human); 100038862 (Mouse); 407781 (Rat)
Alternate Names
BTN9.1; BTNL3; BTNLR; BTNLRButyrophilin-like receptor; butyrophilin-like 3; butyrophilin-like protein 3; COLF4100

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