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Recombinant Mouse BCAM His-tag Protein, CF

Catalog #: 11174-BC Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
11174-BC-050
Recombinant Mouse BCAM His-tag Protein Bioactivity.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse BCAM His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of TE‑85 human osteogenic sarcoma cells. The ED50 for this effect is 18.0-162 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse BCAM protein
Glu26-Ala541, with a C-terminal 6-His tag
Accession #
Q9R069.1
N-terminal Sequence
Analysis
Glu26
Predicted Molecular Mass
58 kDa
SDS-PAGE
65-85 kDa, under reducing conditions.

Product Datasheets

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11174-BC

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11174-BC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity View Larger

Recombinant Mouse BCAM His-tag Protein (Catalog # 11174-BC) supports the adhesion of TE‑85 human osteogenic sarcoma cells. The ED50 for this effect is 18.0-162 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse BCAM His-tag Protein (Catalog # 11174-BC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-85 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BCAM

Basal Cell Adhesion Molecule (BCAM), also known as CD239, is a member of immunoglobulin superfamily protein. BCAM arises from alternate splicing of the Lutheran blood group molecule (Lu) and lacks a 40 amino acid (aa) SH3-containing segment that is present in the cytoplasmic domain of Lutheran (1). Mature mouse BCAM consists of two Ig-like V-type domains and three Ig-like C2-type domains in the extracellular domain (ECD), a transmembrane segment, and a short cytoplasmic domain. Within the ECD, mouse BCAM shares 73% and 91% aa sequence identity with human and rat BCAM, respectively. BCAM is widely expressed in epithelial and endothelial tissues including in the vasculature, kidney glomerulus, small intestine, colon, hair follicle outer root sheath, and basal keratinocytes of the skin during inflammation (2‑5). BCAM is also expressed on vascular and visceral smooth muscle cells and at the neuromuscular junction of skeletal muscle (2, 4, 6, 7). It cooperates with Integrins beta 1 and alpha V beta 3 as an adhesion receptor for Laminins which contain the alpha 5 chain (8, 9). Mouse BCAM binds to both human and mouse Laminin, whereas human BCAM binds to human but not to mouse Laminin (1). BCAM is up‑regulated on carcinomas (particularly ovarian), sarcomas, astrocytomas, and melanomas (2, 3, 5, 6). In contrast to mouse, human BCAM is additionally expressed on erythrocytes and is up‑regulated on these cells in sickle cell disease and polycythemia vera (4, 10, 11). In human erythroid disorders, it mediates increased binding of erythrocytes to Laminin and promotes the formation of erythrocyte-monocyte aggregates (4, 10‑14).

References
  1. Rahuel, C. et al. (1999) Immunogenetics 50:271.
  2. Garin-Chesa, P. et al. (1994) Int. J. Oncol. 5:1261.
  3. Schon, M. et al. (2000) J. Invest. Dermatol. 115:1047.
  4. Rahuel, C. et al. (2008) Am. J. Physiol. Renal Physiol. 294:F393.
  5. Rettig, W.J. et al. (1986) Cancer Res. 46:6406.
  6. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. 85:3110.
  7. Nishimune, H. et al. (2008) J. Cell Biol. 182:1201.
  8. Kikkawa, Y. et al. (2007) J. Biol. Chem. 282:14853.
  9. Vainionpaa, N. et al. (2006) Am. J. Physiol. Cell. Physiol. 290:C764.
  10. Zen, Q. et al. (1999) J. Biol. Chem. 274:728.
  11. Wautier, M.-P. et al. (2007) Blood 110:894.
  12. Udani, M. et al. (1998) J. Clin. Invest. 101:2550.
  13. Chaar, V. et al. (2010) Haematologica 95:1841.
  14. Gauthier, E. et al. (2009) Br. J. Haematol. 148:456.
Long Name
Basal Cell Adhesion Molecule
Entrez Gene IDs
4059 (Human); 57278 (Mouse)
Alternate Names
antigen identified by monoclonal F8; Auberger B antigen; basal cell adhesion molecule (Lu and Au blood groups); basal cell adhesion molecule (Lutheran blood group); basal cell adhesion molecule; B-CAM cell surface glycoprotein; BCAM; B-cell adhesion molecule; CD239 antigen; CD239; F8/G253 antigen; glycoprotein 95kDa; LUAU; Lutheran antigen; Lutheran blood group (Auberger b antigen included); Lutheran blood group glycoprotein; MSK19

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