Recombinant Mouse B7-H3 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10946-B3-050
Recombinant Mouse B7‑H3 Fc Chimera Protein Bioactivity.
2 Images
Product Details
FAQs
Reviews

Recombinant Mouse B7-H3 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity

Measured by its ability to inhibit Mouse CD3 epsilon Antibody induced proliferation of Mouse T cells. The ED50 for this effect is 0.06-0.72 µg/mL.

Source
Mouse myeloma cell line, NS0-derived mouse B7-H3 protein
Mouse B7-H3
(Val29-Phe244)
Accession # Q8VE98.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Val29
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
50 kDa
SDS-PAGE
65-73 kDa, under reducing conditions

Product Datasheets

You must select a language.

x

10946-B3

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10946-B3

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity View Larger

Recombinant Mouse B7-H3 Fc Chimera (Catalog # 10946-B3) inhibits Mouse CD3 epsilon Antibody (Clone # 145-2C11, MAB484) induced proliferation of Mouse T cells. The ED50 for this effect is 0.06-0.72 µg/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse B7‑H3 Fc Chimera Protein (Catalog # 10946-B3) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-73 kDa and 130-150 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: B7-H3

T cells require a signal induced by the engagement of the T cell receptor and a “costimulatory” signal(s) through distinct T cell surface molecules for optimal T cell expansion and activation. Members of the B7 superfamily of counter-receptors were identified by their ability to interact with costimulatory molecules found on the surface of T cells. Members of the B7 superfamily include B7-1 (CD80), B7-2 (CD86), B7-H1 (PD-L1), B7-H2 (B7RP-1), B7-H3, and PD-L2 (1). B7-H3 is expressed at very high levels in immature dendritic cells at moderate levels on mature dendritic cells, LPS stimulated immature dendritic cells and LPS stimulated monocytes, and at low levels on resting monocytes. B7-H3 binds to activated T cells via an as-of-yet identified receptor. B7-H3 co-stimulates proliferation of T cells and interferon-gamma (IFN-gamma ) production and enhances the induction of cytotoxic T cells. B7-H3 shares 20 - 27% amino acid (aa) identity with other B7 family members (2). Murine B7-H3 is a 259 aa protein containing an extracellular domain, a transmembrane domain and a cytoplasmic domain. Mouse and human B7-H3 share 87% aa identity (3).

References
  1. Coyle, A.J. and J.-C. Gutierrez-Ramos (2001) Nature Immunol. 2:203.
  2. Chapoval, A.I. et al. (2001) Nature Immunol. 2:269.
  3. Sun, M. et al. (2002) J. Immunol. 168:6294.
Long Name
B7 Homolog 3
Entrez Gene IDs
80381 (Human); 102657 (Mouse); 315716 (Rat); 102131295 (Cynomolgus Monkey)
Alternate Names
B7H3; B7-H3; B7H34Ig-B7-H3; B7-H3B7 homolog 3; CD276 antigen; CD276 molecule; CD276; Costimulatory molecule

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Mouse B7-H3 Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse B7-H3 Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Mouse B7-H3 Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review