Recombinant Mouse Arylsulfatase G/ARSG Protein, CF
Recombinant Mouse Arylsulfatase G/ARSG Protein, CF Summary
Product Specifications
Gly17-Val525, with a C-terminal 10-His tag
Accession # Q3TYD4
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
4600-SU
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Assay Procedure
- Assay Buffer: 50 mM MES, 250 mM NaCl, pH 5.5
- Recombinant Mouse Arylsulfatase G/ARSG (rmARSG) (Catalog # 4600-SU)
- Substrate: 4-Nitrocatechol Sulfate (PNCS) (Sigma, Catalog # N-7251), 100 mM in deionized water
- Sodium Hydroxide, 1 M stock in deionized water
- 96-well Clear Plate (Costar, Catalog # 92592)
- Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
- Dilute rmARSG to 1 µg/mL in Assay Buffer.
- Dilute Substrate to 2 mM in Assay Buffer.
- Mix 75 μL of 1 µg/mL of rmARSG and 75 µL of 2 mM of Substrate in duplicate. Create a Substrate Blank with 75 µL Assay Buffer and 75 µL Substrate in duplicate. Incubate at 37 °C for 1 hour.
- Stop reaction by adding 150 µL of 0.2 M sodium hydroxide to the reaction vials.
- Load into a clear 96-well plate 200 µL from each reaction vial.
- Read at 510 nm (absorbance) in endpoint mode.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Abs* (OD) x Conversion Factor** (pmol/OD) |
| Incubation time (min) x amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard P-Nitrocatechol (PNC) (Sigma, Catalog # N15553).
- rmARSG: 0.05 µg
- Substrate: 0.5 mM
Reconstitution Calculator
Background: Arylsulfatase G/ARSG
ARSG is a member of the aryl sulfatase family of enzymes with a high degree of amino acid sequence similarity to ARSA and ARSB. ARSG mRNA is expressed in a ubiquitous manner, being detected in most adult and embryonic tissues of the mouse (1).
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