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Recombinant Mouse Activin RIB/ALK-4 Fc Chimera Protein, CF

Catalog #: 1477-AR
1 Citation Datasheet / COA / SDS
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1477-AR-050
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Activin RIB/ALK-4 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rmActivin RIB/Fc Chimera at 1 µg/mL (100 µL/well) can bind rmCripto with a linear range of 0.8‑100 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Activin RIB/ALK-4 protein
Mouse Activin RIB
(Leu32 - Glu126)
Accession #NP_031421		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
NP_031421
N-terminal Sequence
Analysis
Leu32
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
37.2 kDa (monomer)
SDS-PAGE
44 kDa, reducing conditions

Product Datasheets

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1477-AR

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1477-AR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Activin RIB/ALK-4

Activin RIB, also known as ALK-4, is a glycosylated 58 kDa type I transmembrane receptor that belongs to the superfamily of TGF-beta serine/threonine kinase receptors. Activin RIB associates with Activin RIIB to form a receptor complex for activin and inhibin molecules (1). These ligands bind to Activin RIIB which then associates with, and phosphorylates, the cytoplasmic domain of Activin RIB to initiate signal transduction (2, 3). Mature mouse Activin RIB consists of a 103 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 356 aa cytoplasmic region that includes the kinase domain (4). Within the ECD, mouse Activin RIB shares 93% and 98% aa sequence identity with human and rat Activin RIB, respectively. It shares 23% - 38% aa sequence identity with other mouse type I receptors Activin RIA, BMPR-IA, BMPR-IB, and TGF-beta RI. Activin receptor signaling is modulated by the direct interaction of Activin RIB with cripto or inhibin binding protein (5 - 7). Activin RIB is excluded from the signaling complex if Activin RIIB first binds inhibin and betaglycan (8). Activin RIB functions in a wide variety of growth and differentiation processes, including embryonic cell fate and axis determination, cell proliferation, apoptosis, and tumorigenesis (1, 9, 10).

References
  1. Chen, Y.-G. et al. (2006) Exp. Biol. Med. 231:534.
  2. Attisano, L. et al. (1996) Mol. Cell. Biol. 16:1066.
  3. Tsuchida, K. et al. (1995) Endocrinology 136:5493.
  4. Ebner, R. et al. (1993) Science 260:1344.
  5. Chapman, S.C. and T.K. Woodruff (2001) Mol. Endocrinol. 15:668.
  6. Bianco, C. et al. (2002) Mol. Cell. Biol. 22:2586.
  7. Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. 100:5193.
  8. Lewis, K.A. et al. (2000) Nature 404:411.
  9. Gu, Z. et al. (1998) Genes Dev. 12:844.
  10. Chen, Y. et al. (2004) Dev. Biol. 268:280.
Long Name
Activin Receptor IB
Entrez Gene IDs
91 (Human); 11479 (Mouse)
Alternate Names
activin A receptor, type IB; Activin receptor type IB; Activin receptor-like kinase 4; Activin RIB; ActivinRIB; ACTRIB; ACVR1B; ACVRLK4ACTR-IB; ALK4; ALK-4; ALK4activin A receptor, type II-like kinase 4; EC 2.7.11; EC 2.7.11.30; Serine/threonine-protein kinase receptor R2; SKR2activin receptor type-1B

Citation for Recombinant Mouse Activin RIB/ALK-4 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. ALK1 signaling regulates early postnatal lymphatic vessel development.
    Authors: Niessen K, Zhang G, Ridgway JB, Chen H, Yan M
    Blood, 2009-11-10;115(8):1654-61.
    Applications: ELISA (Standard)

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