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Recombinant Mouse Activin RIA/ALK-2 Fc Chimera Protein, CF

Catalog #: 6506-RA Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
6506-RA-100
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse Activin RIA/ALK-2 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Activin RIA Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human BMP‑6 (Catalog # 507-BP) that produces 50% of the optimal binding response is found to be 6-60 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Activin RIA/ALK-2 protein
Mouse Activin RIA
(Met1-Glu123)
Accession # P37172		IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminusC-terminus
Accession #
P37172
N-terminal Sequence
Analysis
Asp23
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
38.4 kDa (monomer)
SDS-PAGE
45-50 kDa, reducing conditions

Product Datasheets

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6506-RA

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6506-RA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Activin RIA/ALK-2

Activin RIA, also known as ALK-2, TSK-7L, SKR1, TSR-I, and ACTR-I, is a glycosylated 65 kDa type I receptor in the TGF-beta serine/threonine kinase receptor family (1-3). Binding of TGF-beta superfamily ligands induces formation of a heterotetrameric complex that contains two chains each of a type I and a type II receptor in multiple combinations. The type II receptors phosphorylate the type I receptors which then phosphorylate and activate Smad signal transduction proteins (1, 2). Activin RIA functions in a wide variety of growth and differentiation processes including gastrulation, skeletal system development, and cardiac morphogenesis (1 4‑7). Mature mouse Activin RIA consists of a 103 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 363 aa cytoplasmic region that includes a Gly/Ser-rich GS box and the kinase domain (8). Within the ECD, mouse Activin RIA shares 99% and 93% aa sequence identity with human and rat Activin RIA, respectively. It shares 24%-26% aa sequence identity with other mouse type I receptors Activin RIB, BMPR-IA, BMPR-IB, and TGF-beta RI. BMP signaling through Activin RIA is enhanced by the direct interaction between Activin RIA and RGM-B/DRAGON, a BMP coreceptor that also associates with other type I and type II receptors (9). Activin RIA can additionally phosphorylate the co‑receptor Endoglin and blocks the inhibitory effect of Endoglin on prostate cancer cell motility (10).

References
  1. Wu, M.Y. and C.S. Hill (2009) Dev. Cell 16:329.
  2. Nickel, J. et al. (2009) Cytokine Growth Factor Rev. 20:367.
  3. de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
  4. Chuva de Sousa Lopes, S.M. et al. (2004) Genes Dev. 18:1838.
  5. Zhang, D. et al. (2003) J. Bone Miner. Res. 18:1593.
  6. van Dinther, M. et al. (2010) J. Bone Miner. Res. 25:1208.
  7. Wang, J. et al. (2005) Dev. Biol. 286:299.
  8. Ebner, R. et al. (1993) Science 260:1344.
  9. Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.
  10. Romero, D. et al. (2010) Carcinogenesis 31:359.
Long Name
Activin Receptor IA
Entrez Gene IDs
90 (Human); 11477 (Mouse)
Alternate Names
activin A receptor, type I; Activin RIA; ActivinRIA; ACTRI; ACVR1; ACVR1A; ACVRLK2; ALK2; ALK-2; FOP; SKR1; TSRI

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