Recombinant Mouse 4-1BB/TNFRSF9 Fc Chimera Protein, CF
Recombinant Mouse 4-1BB/TNFRSF9 Fc Chimera Protein, CF Summary
Product Specifications
| Mouse 4-1BB (Val24-Leu187) Accession # P20334 |
DIEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
937-4B
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: 4-1BB/TNFRSF9/CD137
4-1BB, also known as CD137 and TNFRSF9, is an approximately 30 kDa transmembrane glycoprotein in the TNF receptor superfamily. 4-1BB functions in the development and activation of multiple immune cells (1). Mature mouse 4-1BB consists of a 164 amino acid (aa) extracellular domain (ECD) with four TNFR cysteine‑rich repeats, a 21 aa transmembrane segment, and a 48 aa cytoplasmic domain (2). Within the ECD, mouse 4-1BB shares 60% and 79% aa sequence identity with human and rat 4-1BB, respectively. 4-1BB is expressed as a disulfide-linked homodimer on various populations of activated T cells including CD4+, CD8+, memory CD8+, NKT, and regulatory T cells (3-6) as well as on myeloid and mast cell progenitors, dendritic cells, mast cells, and bacterially infected osteoblasts (7-10). It binds with high affinity to the transmembrane 4-1BB Ligand/TNFSF9 which is expressed on antigen presenting cells and myeloid progenitor cells (7, 11). This interaction co‑stimulates the proliferation, activation, and/or survival of the 4-1BB expressing cell (3-6, 11). It can also enhance the activation-induced cell death of repetitively stimulated T cells (11). Mice lacking 4-1BB show augmented T cell activation, perhaps due to its absence on regulatory T cells (12). 4-1BB can associate with OX40 on activated T cells, forming a complex that responds to either ligand and inhibits Treg and CD8+ T cell proliferation (13). Reverse signaling through 4-1BB Ligand inhibits the development of dendritic cells, B cells, and osteoclasts (7, 10) but supports mature dendritic cell survival and co‑stimulates the proliferation and activation of mast cells (8, 9). 4‑1BB activation enhances CD8+ T cell and NK cell mediated anti-tumor immunity (14). It also contributes to the development of inflammation in high fat diet-induced metabolic syndrome (15). Soluble forms of 4-1BB and 4-1BB Ligand circulate at elevated levels in the serum of rheumatoid arthritis and hematologic cancer patients, respectively (16, 17).
- Kwon, B.S. and S.M. Weissman (1989) Proc. Natl. Acad. Sci. USA 86:1963.
- Wen, T. et al. (2002) J. Immunol. 168:4897.
- Pulle, G. et al. (2006) J. Immunol. 176:2739.
- Zheng, G. et al. (2004) J. Immunol. 173:2428.
- Kim, D. et al. (2008) J. Immunol. 180:2062.
- Lee, S. et al. (2008) Nat. Immunol. 9:917.
- Choi, B.K. et al. (2009) J. Immunol. 182:4107.
- Nishimoto, H. et al. (2005) Blood 106:4241.
- Saito, K. et al. (2004) J. Biol. Chem. 279:13555.
- Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
- Lee, S. et al. (2005) J. Immunol. 174:6803.
- Ma, B.Y. et al. (2005) Blood 106:2002.
- Choi, B.K. et al. (2010) J. Immunol. 185:1404.
- Kim, C. et al. (2011) Diabetes 60:3159.
- Michel, J. et al. (1998) Eur. J. Immunol. 28:290.
- Salih, H.R. et al. (2001) J. Immunol. 167:4059.
Citations for Recombinant Mouse 4-1BB/TNFRSF9 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Trispecific natural killer cell nanoengagers for targeted chemoimmunotherapy
Authors: KM Au, SI Park, AZ Wang
Sci Adv, 2020-07-03;6(27):eaba8564.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
A tumor-targeted trimeric 4-1BB-agonistic antibody induces potent anti-tumor immunity without systemic toxicity
Authors: M Compte, SL Harwood, IG Muñoz, R Navarro, M Zonca, G Perez-Chac, A Erce-Llama, N Merino, A Tapia-Gali, AM Cuesta, K Mikkelsen, E Caleiras, N Nuñez-Prad, MA Aznar, S Lykkemark, J Martínez-T, I Melero, FJ Blanco, J Bernardino, JM Zapata, L Sanz, L Alvarez-Va
Nat Commun, 2018-11-15;9(1):4809.
Species: Mouse
Sample Types: Serum
Applications: ELISA (Capture) -
Cell surface 4-1BBL mediates sequential signaling pathways 'downstream' of TLR and is required for sustained TNF production in macrophages.
Authors: Kang YJ, Kim SO, Shimada S, Otsuka M, Seit-Nebi A, Kwon BS, Watts TH, Han J
Nat. Immunol., 2007-05-13;8(6):601-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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