Recombinant Human Wnt-10b Protein Summary
Product Specifications
Met1-Lys389
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
7196-WN
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
7196-WN/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Wnt-10b
Wnt-10b (also known as Wnt-12) is a 42 ‑ 44 kDa member of the Wnt family of secreted, highly conserved, cysteine-rich glycoproteins that play important roles in vertebrate pattern formation, cell fate decision, axon guidance, and tumor formation (1 ‑ 3). Human Wnt-10b cDNA encodes a 389 amino acid (aa) precursor that contains a 28 aa signal sequence plus a 361 aa mature protein that contains two glycosylation sites, three potential phosphorylation sites, and a potential palmitoylation site (3, 4). Human Wnt-10b shares 97 ‑ 99% aa identity with mouse, rat, equine, porcine, and canine Wnt-10b. Wnt-10b plays a critical role in maintaining mesenchymal stem cells and determining whether they differentiate to adipocytes or osteoblasts (5 ‑ 7). Mouse Wnt-10b deletion produces age‑dependent loss of bone mass due to defective production of osteoblasts, while transgenic over‑expression increases postnatal osteoblast differentiation and inhibits adipocyte differentiation (5 ‑ 7). Ectopic expression of Wnt-10b in an obesity and diabetes‑prone background, such as the ob/ob mouse, inhibits obesity (8). In mouse skeletal muscle, Wnt-10b is expressed inversely with SREBP1c and increases insulin sensitivity (9). In humans, a missense polymorphism is responsible for a malformation of hands and feet, while a C256Y inactivating mutation is associated with severe early-onset obesity (10, 11). Wnt-10b is mainly produced by stem cells and pre-osteoblasts, but also by adult bone marrow CD8+ T lymphocytes stimulated with parathyroid hormone (12). In some hepatocellular carcinomas, Wnt-10b can inhibit cancer cell growth, but in others, it can act synergistically with FGFs to stimulate cell growth (13). Several Wnts, including Wnt-10b, are expressed in both normal and/or malignant colon tissues (14).
- Hardiman, G. et al. (1997) Cytogenet. Cell Genet. 77:278.
- Katoh, M. (2008) Curr. Drug Targets 9:565.
- Hausmann, G. et al. (2007) Nat. Rev. Mol. Cell Biol. 8:331.
- Swiss Prot Accession # O00744.
- Stevens, J.R. et al. (2010) J. Bone Miner. Res. 25:2138.
- Bennett, C.N. et al. (2007) J. Bone Miner. Res. 22:1924.
- Bennett, C.N. et al. (2005) Proc. Natl. Acad. Sci. USA 102:3324.
- Wright, W.S. et al. (2007) Diabetes 56:295.
- Abiola, M. et al. (2009) PLoS ONE 4:e8509.
- Ugur, S.A. and A. Tolun (2008) Hum. Mol. Genet. 17:2644.
- Christodoulides, C. et al. (2006) Diabetologia 49:678.
- Terauchi, M. et al. (2009) Cell Metab. 10:229.
- Yoshikawa, H. et al. (2007) Mol. Biol. Cell 18:4292.
- Holcombe, R.F. et al. (2002) J. Clin. Pathol: Mol. Pathol. 55:220.
Citations for Recombinant Human Wnt-10b Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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CCN6 suppresses metaplastic breast carcinoma by antagonizing WNT/?-catenin signaling to inhibit EZH2-driven EMT
Authors: Gonzalez, ME;Brophy, B;Eido, A;Leonetti, AE;Djomehri, SI;Augimeri, G;Carruthers, NJ;Cavalcante, RG;Giordano, F;Andò, S;Nesvizhskii, AI;Fearon, ER;Kleer, CG;
Cancer research
Species: Human
Sample Types:
Applications: Competition Assay -
Wnt10b protects cardiomyocytes against doxorubicin-induced cell death via MAPK modulation
Authors: Chen, L;Byer, SH;Holder, R;Wu, L;Burkey, K;Shah, Z;
PloS one
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Dissecting specific Wnt components governing osteogenic differentiation potential by human periodontal ligament stem cells through interleukin-6
Authors: Purwaningrum, M;Giachelli, CM;Osathanon, T;Rattanapuchpong, S;Sawangmake, C;
Scientific reports
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Immunocytochemical Analysis of Endogenous Frizzled-(Co-)Receptor Interactions and Rapid Wnt Pathway Activation in Mammalian Cells
Authors: J Neuhaus, A Weimann, M Berndt-Pae
International Journal of Molecular Sciences, 2021-11-08;22(21):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The microRNA-15a-PAI-2 axis in cholangiocarcinoma-associated fibroblasts promotes migration of cancer cells
Authors: P Utaijaratr, K Vaeteewoot, T Tsunematsu, P Jamjantra, S Wongkham, C Pairojkul, N Khuntikeo, N Ishimaru, Y Sirivatana, A Pongpaibul, P Thuwajit, C Thuwajit, Y Kudo
Mol. Cancer, 2018-01-18;17(1):10.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
WNT/?-catenin pathway modulates the TNF-?-induced inflammatory response in bronchial epithelial cells
Authors: J Jang, Y Jung, S Chae, SI Chung, SM Kim, Y Yoon
Biochem. Biophys. Res. Commun, 2017-01-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
LPS-induced inflammatory response is suppressed by Wnt inhibitors, Dickkopf-1 and LGK974
Authors: J Jang, Y Jung, Y Kim, EH Jho, Y Yoon
Sci Rep, 2017-01-27;7(0):41612.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Directed Differentiation of Human Embryonic Stem Cells into Prostate Organoids In Vitro and its Perturbation by Low-Dose Bisphenol A Exposure.
Authors: Calderon-Gierszal E, Prins G
PLoS ONE, 2015-07-29;10(7):e0133238.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity.
Authors: Kilander M, Dahlstrom J, Schulte G
Cell Signal, 2014-05-27;26(9):1943-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Activation of intracellular calcium by multiple Wnt ligands and translocation of beta-catenin into the nucleus: a convergent model of Wnt/Ca2+ and Wnt/beta-catenin pathways.
Authors: Thrasivoulou C, Millar M, Ahmed A
J Biol Chem, 2013-10-24;288(50):35651-9.
Species: Human, Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Whole Cells
Applications: Bioassay
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Reason for Rating: produces repeatable differentiation
