Recombinant Human Wnt-1/sFRP-1 Complex Protein, CF Summary
Product Specifications
| Human Wnt-1 (Ala28-Leu370) Accession # P04628.1 |
| Human sFRP-1 (Ser32-Lys314) Accession # AAB70793.1 | ||
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
11160-WN
| Formulation | Supplied as a 0.2 μm filtered solution in MOPS and NaCl with Trehalose. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Recombinant Human Wnt-1/sFRP-1 Complex (Catalog # 11160-WN) activates Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 25.0-250 ng/mL.
Reconstitution Calculator
Background: Wnt-1
Wnt-1 is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins that are important for normal developmental processes (1). Human Wnt-1 cDNA encodes a 370 amino acid (aa) protein that contains four potential N-linked glycosylation sites plus 23 cysteines (2, 3). When secreted, it exists as a 343 aa glycoprotein with a molecular weight of 42 to 48 kDa. Human Wnt -1 shares a 99.7% aa sequence identity with mouse Wnt-1. Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (4). As intracellular beta -catenin levels rise, beta -catenin binds to TCF/LEF transcription factors leading to expression of Wnt target genes (5). Wnt-1 is known to bind to sFRPs, a family of secreted molecules that contain an N-terminal cysteine-rich domain (CRD) highly similar to the CRDs of the Frizzled family receptors (6,7). More than one molecule of sFRP is suggested to participate in the interaction with each Wnt molecule (7). R&D Systems produces biologically active human Wnt-1 protein in a complex with human sFRP-1, and this Wnt-1/sFRP-1 complex is stable in the physiological buffer without detergent.
- Willert, K. and R. Nusse (2012) Cold Spring Harb. Perspect. Biol. 4:a007864.
- Van Ooyen, A. et al. (1985) EMBO J. 4:2905.
- Burrus, L.W. and A.P. McMahon (1995) Exp. Cell Res. 220:363.
- MacDonald, B.T. and X. He (2012) Cold Spring Harb. Perspect. Biol. 4:a007880.
- Korinek, V. et al. (1997) Science 275:1784.
- Dennis, S. et al. (1999) J. Cell Sci. 112:3815.
- Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.
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