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Recombinant Human USP2 Catalytic Domain Protein, CF

Catalog #: E-504
28 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
E-504-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (28)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human USP2 Catalytic Domain Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human USP2 Catalytic Domain is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP2 Catalytic Domain concentration of 1-5 nM.
Source
E. coli-derived human USP2 protein
Asn259 - Met605
Accession #
O75604.2
Predicted Molecular Mass
40 kDa

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E-504

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-504

Formulation Supplied as a solution in HEPES, NaCl, EDTA and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: USP2

Ubiquitin Specific Peptidase 2 (USP2), also known as UBP41 and USP9, is a cytoplasmic protein that belongs to the peptidase C19 family of deubiquitinating enzymes (1). It is widely expressed and serves to deubiquitinate select target substrates. At least three isoforms have thus far been described: USP2a, also known as USP2-69, USP-41, and USP2-45 (2,3). All isoforms contain the same catalytic core, but show variations within their N- and C-terminal domains (3). USP2a is the canonical isoform. It is 605 amino acids (aa) in length and has a predicted molecular weight of 68 kDa. Human USP2a shares 90% aa sequence identity with the mouse and rat orthologs. USP-41 and USP-45 are 362 and 396 aa in length, respectively, and have predicted molecular weights of 41 and 45 kDa. USP-41 and USP2a have been shown to be critical regulators of apoptosis and cell proliferation. They deubiquitinate poly-ubiquitinated target proteins, such as FAS, MDM2, and Cyclin D1, to promote or block apoptosis, depending on the context (3-9). USP-45, whose expression appears to be regulated by circadian and nutritional cues, is thought to regulate hepatic gluconeogenesis and glucose metabolism (10,11). This recombinant human protein encompasses the catalytic domain of USP2 (aa 259-605).

References
  1. Baek, S.H. et al. (1997) J. Biol. Chem. 272:25560.
  2. Gousseva, N. & R.T. Baker (2003) Gene Expr. 11:163.
  3. Haimerl, F. et al. (2009) J. Biol. Chem. 284:495.
  4. Gewies, A. & S. Grimm (2003) Cancer Res. 63:682.
  5. Graner, E. et al. (2004) Cancer Cell 5:253.
  6. Stevenson, L.F. et al. (2007) EMBO J. 26:976.
  7. Shan, J. et al. (2009) Mol. Cell 36:469.
  8. Mahul-Mellier, A.L. et al. (2012) Cell Death Differ. 19:891.
  9. Mahul-Mellier, A.L. et al. (2012) Biochim. Biophys. Acta. 1823:1353.
  10. Molusky, M.M. et al. (2012) Diabetes 61:1025.
  11. Molusky, M.M. et al. (2012) PLoS One 7:e47970.
Long Name
Ubiquitin-specific Protease 2
Entrez Gene IDs
9099 (Human); 53376 (Mouse); 115771 (Rat)
Alternate Names
Deubiquitinating enzyme 2; EC 3.1.2.15; EC 3.4.19.12,41 kDa ubiquitin-specific protease; ubiquitin specific peptidase 2; ubiquitin specific protease 12; ubiquitin specific protease 2; ubiquitin specific protease 9; ubiquitin thioesterase 2; Ubiquitin thiolesterase 2; Ubiquitin-specific-processing protease 2; UBP41; UBP41ubiquitin carboxyl-terminal hydrolase 2; USP2; USP9

Citations for Recombinant Human USP2 Catalytic Domain Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. The IRAK1/IRF5 axis initiates IL-12 response by dendritic cells and control of Toxoplasma gondii infection
    Authors: Pereira, M;Ramalho, T;Andrade, WA;Durso, DF;Souza, MC;Fitzgerald, KA;Golenbock, DT;Silverman, N;Gazzinelli, RT;
    Cell reports
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Sde Proteins Coordinate Ubiquitin Utilization and Phosphoribosylation to Promote Establishment and Maintenance of the Legionella Replication Vacuole
    Authors: Isberg, R;Kotewicz, K;Zheng, M;Kim, S;Tai, A;
    Research square
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. Cooperative assembly of p97 complexes involved in replication termination
    Authors: OV Kochenova, S Mukkavalli, M Raman, JC Walter
    Nature Communications, 2022-11-03;13(1):6591.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  4. Ubiquitination of CLIP-170 family protein restrains polarized growth upon DNA replication stress
    Authors: X Wang, F Zheng, YY Yi, GY Wang, LX Hong, D McCollum, C Fu, Y Wang, QW Jin
    Nature Communications, 2022-09-22;13(1):5565.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  5. E3 ligase HUWE1 promotes PDGF D-mediated osteoblastic differentiation of mesenchymal stem cells by effecting polyubiquitination of beta-PDGFR
    Authors: T Pham, AJ Najy, HC Kim
    The Journal of Biological Chemistry, 2022-04-25;0(0):101981.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. A trio of ubiquitin ligases sequentially drives ubiquitylation and autophagic degradation of dysfunctional yeast proteasomes
    Authors: RS Marshall, RD Vierstra
    Cell Reports, 2022-03-15;38(11):110535.
    Applications: Bioassay
  7. Structure of CRL2Lrr1, the E3 ubiquitin ligase that promotes DNA replication termination in vertebrates
    Authors: H Zhou, MS Zaher, JC Walter, A Brown
    Nucleic Acids Research, 2021-12-16;49(22):13194-13206.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  8. Ubiquitylation of lipopolysaccharide by RNF213 during bacterial infection
    Authors: EG Otten, E Werner, A Crespillo-, KB Boyle, V Dharamdasa, C Pathe, B Santhanam, F Randow
    Nature, 2021-05-19;0(0):.
    Species: Bacteria - Salmonella typhimurium
    Sample Types: Cell Lysates
    Applications: Bioassay
  9. The deubiquitinase TRABID stabilizes the K29/K48-specific E3 ubiquitin ligase HECTD1
    Authors: LD Harris, J Le Pen, N Scholz, J Mieszczane, N Vaughan, S Davis, G Berridge, BM Kessler, M Bienz, JDF Licchesi
    The Journal of Biological Chemistry, 2021-01-08;296(0):100246.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  10. The nucleosome acidic patch and H2A ubiquitination underlie mSWI/SNF recruitment in synovial sarcoma
    Authors: MJ McBride, N Mashtalir, EB Winter, HT Dao, M Filipovski, AR D'Avino, HS Seo, NT Umbreit, R St Pierre, AM Valencia, K Qian, HJ Zullow, JD Jaffe, S Dhe-Pagano, TW Muir, C Kadoch
    Nat. Struct. Mol. Biol., 2020-08-03;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Manumycin polyketides act as molecular glues between UBR7 and P53
    Authors: Y Isobe, M Okumura, LM McGregor, SM Brittain, MD Jones, X Liang, R White, W Forrester, JM McKenna, JA Tallarico, M Schirle, TJ Maimone, DK Nomura
    Nat. Chem. Biol., 2020-06-22;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  12. Cockayne syndrome group A and B proteins function in rRNA transcription through nucleolin regulation
    Authors: MN Okur, JH Lee, W Osmani, R Kimura, TG Demarest, DL Croteau, VA Bohr
    Nucleic Acids Res., 2020-03-18;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  13. Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase
    Authors: M Fottner, AD Brunner, V Bittl, D Horn-Ghetk, A Jussupow, VRI Kaila, A Bremm, K Lang
    Nat. Chem. Biol., 2019-02-15;15(3):276-284.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Ubiquitination
  14. Correcting errors in the BRCA1 warning system
    Authors: Y Liang, WJ Dearnaley, NA Alden, MJ Solares, BL Gilmore, KJ Pridham, AC Varano, Z Sheng, E Alli, DF Kelly
    DNA Repair (Amst.), 2018-11-22;73(0):120-128.
    Applications: Bioassay
  15. Translesion polymerase kappa-dependent DNA synthesis underlies replication fork recovery
    Authors: P Tonzi, Y Yin, CWT Lee, E Rothenberg, TT Huang
    Elife, 2018-11-13;7(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  16. Androgen receptor polyglutamine expansion drives age-dependent quality control defects and muscle dysfunction
    Authors: SR Nath, Z Yu, TA Gipson, GB Marsh, E Yoshidome, DM Robins, SV Todi, DE Housman, AP Lieberman
    J. Clin. Invest., 2018-07-23;0(0):.
    Applications: Bioassay
  17. CHIP E3 ligase mediates proteasomal degradation of the proliferation regulatory protein ALDH1L1 during the transition of NIH3T3 fibroblasts from G0/G1 to S-phase
    Authors: QA Khan, P Pediaditak, Y Malakhau, A Esmaeilnia, Z Ashkavand, V Sereda, NI Krupenko, SA Krupenko
    PLoS ONE, 2018-07-06;13(7):e0199699.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  18. Expression and Regulation of Deubiquitinase-Resistant, Unanchored Ubiquitin Chains in Drosophila
    Authors: JR Blount, K Libohova, GB Marsh, JR Sutton, SV Todi
    Sci Rep, 2018-05-31;8(1):8513.
    Applications: Bioassay
  19. Mfn2 ubiquitination by PINK1/parkin gates the p97-dependent release of ER from mitochondria to drive mitophagy
    Authors: GL McLelland, T Goiran, W Yi, G Dorval, CX Chen, ND Lauinger, AI Krahn, S Valimehr, A Rakovic, I Rouiller, TM Durcan, JF Trempe, EA Fon
    Elife, 2018-04-20;7(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  20. A VCP inhibitor substrate trapping approach (VISTA) enables proteomic profiling of endogenous ERAD substrates
    Authors: EY Huang, M To, E Tran, LTA Dionisio, HJ Cho, KLM Baney, CI Pataki, JA Olzmann
    Mol. Biol. Cell, 2018-03-22;0(0):.
    Applications: Bioassay
  21. Rapid induction of p62 and GABARAPL1 upon proteasome inhibition promotes survival before autophagy activation
    Authors: Z Sha, HM Schnell, K Ruoff, A Goldberg
    J. Cell Biol., 2018-03-13;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  22. Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression
    Authors: X Liu, JM Reitsma, JL Mamrosh, Y Zhang, R Straube, RJ Deshaies
    Mol. Cell, 2018-03-01;69(5):773-786.e6.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  23. ERAD-dependent control of the Wnt secretory factor Evi
    Authors: K Glaeser, M Urban, E Fenech, O Voloshanen, D Kranz, F Lari, JC Christians, M Boutros
    EMBO J., 2018-01-29;37(4):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  24. Phosphorylation of the type II transmembrane serine protease, TMPRSS13 in Hepatocyte Growth Factor Activator Inhibitor-1 and 2-mediated cell surface localization
    Authors: AS Murray, FA Varela, TE Hyland, AJ Schoenbeck, JM White, LM Tanabe, SV Todi, K List
    J. Biol. Chem., 2017-07-14;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Keap1/Cullin3 Modulates p62/SQSTM1 Activity via UBA Domain Ubiquitination
    Authors: Y Lee, TF Chou, SK Pittman, AL Keith, B Razani, CC Weihl
    Cell Rep, 2017-04-04;19(1):188-202.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Bioassay
  26. p97/VCP promotes degradation of CRBN substrate glutamine synthetase and neosubstrates
    Authors: TV Nguyen, J Li, CJ Lu, JL Mamrosh, G Lu, BE Cathers, RJ Deshaies
    Proc. Natl. Acad. Sci. U.S.A, 2017-03-20;0(0):.
    Applications: Bioassay
  27. An inhibitory mono-ubiquitylation of the Drosophila initiator caspase Dronc functions in both apoptotic and non-apoptotic pathways
    Authors: HE Kamber Kay, M Ditzel, P Meier, A Bergmann
    PLoS Genet, 2017-02-16;13(2):e1006438.
    Species: Drosophila
    Sample Types: Protein
    Applications: Bioassay
  28. A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses.
    Authors: Liang, Qin, Dexheimer, Thomas S, Zhang, Ping, Rosenthal, Andrew S, Villamil, Mark A, You, Changjun, Zhang, Qiuting, Chen, Junjun, Ott, Christin, Sun, Hongmao, Luci, Diane K, Yuan, Bifeng, Simeonov, Anton, Jadhav, Ajit, Xiao, Hui, Wang, Yinsheng, Maloney, David J, Zhuang, Zhihao
    Nat Chem Biol, 2014-02-16;10(4):298-304.
    Applications: Bioassay

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