Recombinant Human TMED1 Fc Chimera Protein, CF Summary
Product Specifications
| Human TMED1 (Ala24-Asn194) Accession # Q13445 | IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2243-TM
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: |
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Scientific Data
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When Recombinant TMED1 Fc Chimera (Catalog # 2243-TM) is coated at 0.5 µg/mL, Recombinant Human ST2/IL-1 R4 Fc Chimera (Catalog # 523-ST) binds with an ED50 of 0.6-3.6 µg/mL.
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2 μg/lane of Recombinant Human TMED1 Fc was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 56-62 kDa and 110-120 kDa, respectively.
Reconstitution Calculator
Background: TMED1
TMED1 (Transmembrane Emp24 domain-containing protein 1) is a member of the TMED family of proteins (gene name TMED1). The TMED family of proteins are localized to membranes of the early secretory pathway, including the endoplasmic reticulum and Golgi, and function in vesicular protein trafficking (1, 2). TMED1 is a 59 kDa monomer and has been reported to exist as homodimer (3). TMED1 is composed of a 23 amino acid (aa) signal sequence, a 171 aa extra cellular domain, a 21 aa transmembrane domain, and a 12 aa cytoplasmic domain. The extracellular domain contains an 83 aa GOLD (Golgi Dynamics) domain, and COPI and COPII binding motifs are found in the cytoplasmic domain (1-3, 5). Human TMED1 shares 97% sequence identity with mouse, bovine, and rat homologs within the 171 aa extracellular domain. The beta -strand-rich GOLD domain has been specifically identified to be involved in intracellular protein trafficking (1, 4, 5). TMED1 is important in regulating innate immune signaling through its interaction with ST2L. Specifically, the GOLD domain in TMED1 interacts with the TIR domain of ST2L, a receptor for IL‑33 (1). This interaction promotes ST2L association with IL-33, allowing downstream signaling cascade activating MAP kinases, p38, and JNK (1, 6). Studies have shown knockdown of TMED-1 in HUVECs impairs the IL-33 induced response resulting in reduction of IL-6 and IL-8 productions (1).
- Connolly, D. et al. (2013) J Biol Chem. 288:5616.
- Gour, N. and Lajoie, S. (2018) Curr Allergy Ashma Rep. 16:65.
- Jenne, N. (2002) J Biol Chem. 277:46504.
- Anantharaman, V. and Aravind, L. (2002) Genome Biol. 3:research0023
- Gomez-Navarro, N. and Miller, E. (2016) J Cell Biol. 215:769.
- Hardman, C. and Ogg, G. (2016). Curr Opin Immunol. 42:16.
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