Recombinant Human Testican 1/SPOCK1 Protein, CF Summary
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Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2327-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
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Background: Testican 1/SPOCK1
Testican 1, encoded by the SPOCK1 gene, is a proteoglycan first identified in human seminal plasma (1). The cDNAs from the human testis and mouse brain indicate 95% identity between the deduced amino acid sequences from the two species, predicting a conserved function (2, 3). Human Testican 1 is able to inhibit attachment of Neuro-2a cells and their ability to form neurite extensions (4). At R&D Systems, recombinant human (rh) Testican 1 has also been shown to be able to enhance neurite outgrowth of E18 rat embryonic hippocampal neurons. Testican 1 contains Ca2+-binding domain and the C-terminal acidic domain with putative glycosaminoglycan attachment sites (5). In addition, it contains three potential inhibitory domains targeted toward three different classes of proteases, metallo, cysteine and serine proteases. The N-terminal region, which is unique to testicans, is responsible for the inhibition of testican 1 towards MMP-14 (MT1-MMP, a metalloprotease) activation of MMP-2 (6). The thyropin domain may be responsible for the inhibition of testican 1 towards cathepsin L, a cysteine protease (5). The follistatin-like domain with a six cysteine Kazal-like motif may inhibit serine proteases. The purified rhTestican 1 is capable of inhibiting rhMMP-14 and rhCathepsin L (Catalog # 918-MP and 952-CY) in assays using the fluorogenic peptide substrates (Catalog # ES001 and ES008).
- Bonnet, F. et al. (1992) Biochem. J. 288:565.
- Alliel, P.M. et al. (1993) Eur. J. Biochem. 214:347.
- Bonnet, F. et al. (1996) J. Biol. Chem. 271:565.
- Marr, H.S. and C.-J.S Edgell (2003) Matrix Biol. 22:259.
- Bocock, J.P. et al. (2003) Eur. J. Biochem. 270:4008.
- Nakada, M. et al. (2001) Cancer Res. 61:8896.
Citations for Recombinant Human Testican 1/SPOCK1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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SPOCK1 overexpression induced by platelet-derived growth factor-BB promotes hepatic stellate cell activation and liver fibrosis through the integrin alpha5beta1/PI3K/Akt signaling pathway
Authors: Z Du, Z Lin, Z Wang, D Liu, D Tian, L Xia
Lab. Invest., 2020-04-14;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Broad regulation of matrix and adhesion molecules in THP-1 human macrophages by nitroglycerin.
Authors: Krishnatry AS, Brazeau DA, Fung HL
Nitric Oxide, 2009-10-15;22(1):11-7.
Applications: Western Blot
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