Recombinant Human Syndecan-1 Protein, CF Summary
Product Specifications
Gln18-Glu251, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2780-SD
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Syndecan-1/CD138
Syndecan-1, designated CD138, is a dimeric type I transmembrane (TM) protein that belongs to the syndecan family of Type 1 transmembrane proteins (1, 2). The four syndecan family members are major carriers of heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans (GAGs) that have different expression patterns and extracellular sequences. Syndecan-1 forms weak non-covalent homodimers, or heterodimers with Syndecan-2 or -3, through interactions of the transmembrane domain (3). It is synthesized as a 310 amino acid (aa) precursor with a 17 aa signal sequence, a 234 aa extracellular domain (ECD) that includes three closely-spaced consensus Ser-Gly HS attachment sites near the N-terminus, a 25 aa TM segment, and a 34 aa cytoplasmic region that includes a PDZ binding motif with a tyrosine phosphorylation site. The ECD is variably modified by GAGs, producing molecular weights of 120 - 200 kDa for native Syndecan-1. Soluble forms are shed via proteolytic cleavage. Human Syndecan-1 ECD shares 65 - 71% aa identity with the ECD of rat, mouse, canine, equine and bovine Syndecan-1. Syndecan-1 shows highest expression on epithelial cells such as keratinocytes, and terminally differentiated B cells such as plasma cells (4, 5). It aids wound healing in skin, cornea, and heart following myocardial infarction by promoting re-epithelialization, migration, and collagen deposition (4 - 8). It binds chemokines, creating chemotactic gradients when shed, but also binds and modulates integrins to control the influx of leukocytes (5, 7, 9). The net effect is to allow, but limit, inflammation. In myeloma and other cancers, shedding of Syndecan-1 can facilitate growth, angiogenesis and metastasis (10 - 12). Growth factors, such as FGFs and HGF, bind GAG chains and use Syndecan-1 as a coreceptor (12, 13). The GAG chains may also be used by a variety of viruses and bacteria for cell adhesion and uptake (4).
- Tkachenko, E. et al. (2005) Circ. Res. 96:488.
- Mali, M. et al. (1990) J. Biol. Chem. 265:6884.
- Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
- Fears, C.Y. and A. Woods (2006) Matrix Biol. 25:443.
- Stepp, M.A. et al. (2002) J. Cell Sci. 115:4517.
- Ojeh, N. et al. (2008) J. Invest. Dermatol. 128:26.
- Stepp, M.A. et al. (2007) J. Cell Sci. 120:2851.
- Vanhoutte, D. et al. (2007) Circulation 115:475.
- Li, Q. et al. (2002) Cell 111:635.
- Beauvais, D.M. et al. (2009) J. Exp. Med. 206:691.
- Yang, Y. et al. (2007) J. Biol. Chem. 282:13326.
- Derksen, P.W.B. et al. (2002) Blood 99:1405.
- Su, G. et al. (2007) J. Biol. Chem. 282:14906.
Citations for Recombinant Human Syndecan-1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Chain-specificity of laminin ?1-5 LG45 modules in the recognition of carbohydrate-linked receptors and intramolecular binding
Authors: Matsunuma, M;Kan, R;Yamada, Y;Hamada, K;Kanagawa, M;Nomizu, M;Kikkawa, Y;
Scientific reports
Species: Human
Sample Types:
Applications: Bioassay -
Glycocalyx components affect platelet function, whole blood coagulation, and fibrinolysis: an in vitro study suggesting a link to trauma-induced coagulopathy
Authors: MW Britten, L Lümers, K Tominaga, J Peters, D Dirkmann
BMC anesthesiology, 2021-03-19;21(1):83.
Species: Human
Sample Types: Reference Standard
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Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases
Authors: AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano
Sci Rep, 2020-07-31;10(1):12912.
Species: Snake
Sample Types: Protein
Applications: Bioassay -
The stressed vascular barrier and coagulation - The impact of key glycocalyx components on in vitro clot formation
Authors: JI Buchheim, MC Enzinger, A Choukèr, M Bruegel, L Holdt, M Rehm
Thromb. Res., 2019-12-28;186(0):93-102.
Species: Human
Sample Types: Whole Blood
Applications: Activation -
Dengue Virus NS1 Disrupts the Endothelial Glycocalyx, Leading to Hyperpermeability
PLoS Pathog, 2016-07-14;12(7):e1005738.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
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Average Rating: 4.8 (Based on 4 Reviews)
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Reason for Rating: used in spike-and-recovery eval in serum
Reason for Rating: Works with correspoding Duoset ELISA but needs to be measured with kit's calibration curve.
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