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Recombinant Human SIRP delta Fc Chimera Protein, CF

Catalog #: 10138-SB Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
10138-SB-050
Recombinant Human SIRP delta Fc Chimera Protein Binding Activity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human SIRP delta Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human SIRP delta Fc Chimera (Catalog # 10138-SB) is immobilized at 1 µg/mL (100 µL/well), recombinant human DIRAS2 binds with an ED50 of 0.15-0.9 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human SIRP delta protein
Human SIRP delta
(Phe30-Arg197)
Accession # Q9H106		IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
Q9H106
N-terminal Sequence
Analysis
Phe30
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
45 kDa
SDS-PAGE
50-60 kDa, under reducing conditions

Product Datasheets

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10138-SB

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10138-SB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human SIRP delta Fc Chimera Protein Binding Activity View Larger

When Recombinant Human SIRP delta Fc Chimera (Catalog # 10138-SB) is immobilized at 1 µg/mL, recombinant human DIRAS2 binds with an ED50 of 0.15-0.9 μg/mL.

SDS-PAGE Recombinant Human SIRP delta Fc Chimera Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Human SIRP delta Fc Chimera (Catalog # 10138-SB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-60 kDa and 100-120 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SIRP delta

SIRPD (Signal Regulatory Protein Delta), also know as Protein Tyrosine Phosphatase non-Receptor Type Substrate 1-l Like 2 (PTPNS1L2), is member of the signal regulatory proteins (SIRPS) family (1). SIRPD contains a 168 amino acid Ig-like domain that is characteristic of other SIRP family members (1). Unlike other members of SIRPS family, SIRPD lacks the transmemberane region, and is secreted (2). Murine homologs of SIRPD are not characterized. Expression sequence tag analysis suggests that SIRPD may be expressed in sperm cells and respiratory tissue (2). Using BioPlex 2.0 (Biophysical Interactions of ORFeome-derived complexes) high-throughput affinity purification–mass spectrometry (AP–MS) analysis to identify probable protein–protein interactions, several candidate SIRPD interactions were found including DIRAS2 (3). In-house testing indicates SIRPD can interact with DIRAS2.

References
  1. Van den Berg, T.K. et al. (2005) J. Immunol. 175:7788.
  2. Van Beek, E.M. et al. (2005) J Immunol. 175:7781.
  3. Huttlin, E.L. et al. (2017) Nature 545:505.
Long Name
Signal-regulatory Protein delta
Entrez Gene IDs
128646 (Human)
Alternate Names
dJ576H24.4; Protein tyrosine phosphatase non-receptor type substrate 1-like 2; protein tyrosine phosphatase, non-receptor type substrate 1-like 2; PTPNS1L2; signal-regulatory protein delta; SIRP delta; SIRPD; SIRP-Delta

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