Recombinant Human Serpin E2/PN1 Protein, CF Summary
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Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2980-PI
| Formulation | Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Reconstitution Calculator
Background: Serpin E2/PN1
Serpin E2, also known as protease nexin I or glial-derived nexin (GDN), is a member of the Serpin superfamily of the serine protease inhibitors (1). Serpin E2 is a potent inhibitor of thrombin, plasmin and plasminogen activators (2). It is differentially expressed during neuronal differentiation and is able to transform human embryonic kidney cells into neuron-like cells (3). Its over‑expression in mice leads to progressive neuronal and motor dysfunction in these animals (4). It is also over‑expressed in the majority of pancreatic carcinoma as well as gastric and colorectal cancer samples whereas it is weakly expressed in all normal pancreas and chronic pancreatitis tissue samples (5). It plays an important role in controlling male fertility because its knockout male mice show a marked impairment in fertility from the onset of sexual maturity and its abnormal expression is found in the semen of men with seminal dysfunction (6). The deduced amino acid sequence of rhSerpin E2 is the same as that in NP_001130000, which predicts Arg329 in its 397 amino acid residues (7). An alternatively splice form predicts Thr-Gly at positions 329 and 330 in its 398 amino acid sequence (8-10).
- Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
- Rossignol, P. et al. (2004) J. Biol. Chem. 279:10346.
- Lin, H.J. et al. (2005) Int. J. Dev. Neurosci. 23:9.
- Meins, M. et al. (2001) J. Neurosci. 21:8830.
- Buchholz, M. et al. (2003) Cancer Res. 63:4945.
- Murer, V. et al. (2001) Proc. Natl. Acad. Sci. USA 98:3029.
- Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
- Sommer, J. et al. (1987) Biochemistry 26:6407.
- Gloor, S. et al. (1986) Cell 47:687.
- McGrogan, M. et al. (1988) Bio/Technology 6:172.
Citations for Recombinant Human Serpin E2/PN1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Loss of HAI-2 in mice with decreased prostasin activity leads to an early-onset intestinal failure resembling congenital tufting enteropathy
Authors: R Szabo, TH Bugge
PLoS ONE, 2018-04-04;13(4):e0194660.
Applications: Bioassay -
The Involvement of Protease Nexin-1 (PN1) in the Pathogenesis of Intervertebral Disc (IVD) Degeneration
Sci Rep, 2016-07-27;6(0):30563.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
SERPINE2 Inhibits IL-1alpha-Induced MMP-13 Expression in Human Chondrocytes: Involvement of ERK/NF-kappaB/AP-1 Pathways.
Authors: Santoro A, Conde J, Scotece M, Abella V, Lois A, Lopez V, Pino J, Gomez R, Gomez-Reino J, Gualillo O
PLoS ONE, 2015-08-25;10(8):e0135979.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Matriptase initiates activation of epidermal pro-kallikrein and disease onset in a mouse model of Netherton syndrome.
Authors: Sales KU, Masedunskas A, Bey AL
Nat. Genet., 2010-07-25;42(8):676-83.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Prostasin expression is regulated by airway surface liquid volume and is increased in cystic fibrosis.
Authors: Myerburg MM, McKenna EE, Luke CJ, Frizzell RA, Kleyman TR, Pilewski JM
Am. J. Physiol. Lung Cell Mol. Physiol., 2008-02-29;294(5):L932-41.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Autosomal ichthyosis with hypotrichosis syndrome displays low matriptase proteolytic activity and is phenocopied in ST14 hypomorphic mice.
Authors: List K, Currie B, Scharschmidt TC, Szabo R, Shireman J, Molinolo A, Cravatt BF, Segre J, Bugge TH
J. Biol. Chem., 2007-10-16;282(50):36714-23.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Bioassay
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