Recombinant Human RELT/TNFRSF19L Fc Chimera Protein, CF

Recombinant Human RELT/TNFRSF19L Fc Chimera Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: RELT/TNFRSF19L

RELT (Receptor Expressed in Lymphoid Tissues) is a 46 kDa (predicted) type I transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily, designated TNFRSF19-like (TNFRSF19L) (1, 2). It is primarily expressed in hematopoietic tissues and peripheral blood leukocytes (2, 3). Human RELT cDNA encodes 430 amino acids (aa), including a 25 aa signal peptide, a 137 aa extracellular domain containing a TNF receptor cysteine-rich domain and a potential N‑linked glycosylation site, a 21 aa transmembrane domain and a 247 aa cytoplasmic region that lacks a death domain. Within the ECD, human RELT shares 78%, 75%, 75%, 78% and 72% aa sequence homology with mouse, rat, canine, porcine and bovine RELT, respectively. Among TNFRSF members, the RELT extracellular domain is most closely related to that of TROY/TNFRSF19 and OX40 (2). Neither human nor mouse RELT bind any of the ~19 TNF superfamily ligands that have been tested (1). Two related transmembrane proteins, RELL1 and RELL2, have been identified in both human and mouse (3). RELL1 and 2 can interact with and are coexpressed with RELT, and are thought to modulate its signaling (3-5). Intracellularly, RELT has been shown to bind the adaptor protein TRAF-1 and activate the NF‑ kappa B pathway, the phospholipid scramblase PLSCR1, and oxidative stress responsive proteins OSR1 (2-5). Another investigator notes association and signaling through SPAK, but not TRAF or NF kappa B (6). When overexpressed in HEK-293 cells, RELT induces p38 and JNK signaling and activates apoptosis (4-6). Immobilized RELT can also costimulate T-cell proliferation in the presence of CD3 signaling (2).

Related Research Areas

• Co-stimulatory and Co-inhibitory Molecules
• Regulation of Apoptosis by TNF Superfamily Members
• Regulation of T Cell Co-stimulation by TNF Superfamily Members
• TNF Superfamily
• TNF Superfamily Receptors