Recombinant Human PSGL-1/CD162 Fc Chimera Protein, CF Summary
Why choose R&D Systems PSGL-1 Protein?
- Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 95%.
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Product Specifications
| Human PSGL-1 (Gln42-Val295) Accession # AAC50061 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Gln42
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3345-PS
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Recombinant Human PSGL-1/CD162 Fc Chimera (Catalog # 3345-PS) supports the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin. The ED50 for this effect is 0.04-0.32 μg/mL.
Reconstitution Calculator
Background: PSGL-1/CD162
PSGL-1 (P-Selectin Glycoprotein Ligand-1, designated CD162), is a 110-120 kDa mucin-type glycoprotein that plays a key role in leukocyte adhesion (1-3). Human PSGL-1 cDNA encodes 412 amino acids (aa) including a 17 aa signal sequence, a 24 aa propeptide, a 279 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 71 aa cytoplasmic region (1). The ECD of human PSGL-1 contains 16 decameric repeats in the A allele (long form), which is expressed by 65-80% of the population. Alleles B and C contain 15 and 14 repeats, respectively, and mouse PSGL-1 contains 10-12 repeats (1, 3). Shorter forms may show weaker binding to P-selectin (9). Human PSGL-1 ECD shares less than 50% aa sequence identity with mouse, equine, canine and porcine PSGL-1 ECD. However, all share structural motifs and functions (1-3). The mature PSGL-1 (aa 42-412) is expressed as a disulfide-linked homodimer that signals intracellularly and promotes integrin activation (2‑4). Ligand binding relies on presence of O-glycosylation/sialylated Lewis X (LeX) and tyrosine sulfation within aa 42-57 (1, 5-7). Sulfation and glycosylation, which are constitutive on neutrophils and induced with activation on T cells, are essential for binding to P-selectin and L-selectin (3-6). E-Selectin requires only glycosylation (3). Proteases such as neutrophil elastase and cathepsin G can cleave within this region, impacting binding affinity (3, 8). Cleaved, soluble ectodomains of PSGL-1 are also known (3). PSGL-1 is found on virtually all leukocytes, dendritic cells, platelets, and some endothelial cells (3). It is primarily responsible for early events in extravasation, especially rolling adhesion of leukocytes to vascular endothelium (3, 4). In addition, PGSL-1 binds chemokines such as CCL19, CCL21 and CCL27, promoting chemotaxis of hematopoietic stem cells and plasma cells to the bone marrow and T cell homing to lymphoid organs (3, 10, 11). Recombinant PSGL-1 ECD interferes with this chemotaxis (13). PSGL-1 binds von Willebrand factor in endothelial-derived Weibel-Palade bodies, and the chondroitin sulfate, versican (3). It is present with Tissue Factor in pro-thrombotic microparticles from membranes of monocytes and cancer cells, and is required for particle shedding and fusion with activated platelets (12, 13).
- Yang, J. et al. (1999) Thromb. Haemost. 81:1.
- Sako, D. et al. (1993) Cell 75:1179.
- Carlow, D.A. et al. (2009) Immunol. Rev. 230:75.
- Zarbock, A. et al. (2011) Blood 278:37.
- Bernimoulin, M.P. et al. (2003) J. Biol. Chem. 278:37.
- Sako, D. et al. (1995) Cell 83:323.
- Xia, L. et al. (2003) Blood 101:552.
- Gardiner, E.E. et al. (2001) Blood 98:1440.
- Lozano, M.L. et al. (2001) Br. J. Haematol. 115:969.
- Veerman, K.M. et al. (2007) Nat. Immunol. 8:532.
- Hirata, T. et al. (2004) J. Biol. Chem. 279:51775.
- del Conde, I. et al. (2005) Blood 106:1604.
- Thomas, G.M. et al. (2009) J Exp. Med. 206:1913.
Citations for Recombinant Human PSGL-1/CD162 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Oligomer-guided recognition of two fucan sulfate from Bohadschia argus and inhibition of P-selectin binding to its ligand
Authors: Ruzemaimaiti, D;Sun, H;Zhang, J;Xu, C;Chen, L;Yin, R;Zhao, J;
Carbohydrate polymers
Species: N/A
Sample Types: Carbohydrates
Applications: ELISA Detection -
A previously uncharacterized O-glycopeptidase from Akkermansia muciniphila requires the Tn-antigen for cleavage of the peptide bond
Authors: BJ Medley, L Leclaire, N Thompson, KE Mahoney, B Pluvinage, MAH Parson, JE Burke, S Malaker, W Wakarchuk, AB Boraston
The Journal of Biological Chemistry, 2022-08-30;0(0):102439.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Therapeutic implementation in arterial thrombosis with pulmonary administration of fucoidan microparticles containing acetylsalicylic acid
Authors: MS Saito, KC Zatta, PC Sathler, PS Furtado, N C O Miguel, FS Frattani, M Berger, V Lavayen, AR Pohlmann, SS Guterres
International journal of pharmaceutics, 2022-05-25;622(0):121841.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Structure-guided mutagenesis of a mucin-selective metalloprotease from Akkermansia muciniphila alters substrate preferences
Authors: DJ Shon, D Fernandez, NM Riley, MJ Ferracane, CR Bertozzi
The Journal of Biological Chemistry, 2022-04-09;0(0):101917.
Species: Akkermansia muciniphila
Sample Types: Protein
Applications: Bioassay -
Quantification of fast molecular adhesion by fluorescence footprinting
Authors: AB Yasunaga, ITS Li
Science Advances, 2021-08-18;7(34):.
Species: Human
Sample Types: Recombinant Proteins
Applications: Bioassay -
The vimentin rod domain blocks P-selectin-P-selectin glycoprotein ligand 1 interactions to attenuate leukocyte adhesion to inflamed endothelium
Authors: FW Lam, CA Brown, C Valladolid, DC Emebo, TG Palzkill, MA Cruz
PLoS ONE, 2020-10-13;15(10):e0240164.
Species: N/A
Sample Types: Complex Sample Type
Applications: Bioassay -
An engineered antibody binds a distinct epitope and is a potent inhibitor of murine and human VISTA
Authors: N Mehta, S Maddineni, RL Kelly, RB Lee, SA Hunter, JL Silberstei, RA Parra Sper, CL Miller, A Rabe, L Labanieh, JR Cochran
Scientific Reports, 2020-09-16;10(1):15171.
Species: Human
Sample Types: Peptide
Applications: Bioassay -
The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins
Authors: SA Malaker, K Pedram, MJ Ferracane, BA Bensing, V Krishnan, C Pett, J Yu, EC Woods, JR Kramer, U Westerlind, O Dorigo, CR Bertozzi
Proc. Natl. Acad. Sci. U.S.A., 2019-03-25;0(0):.
Species: Bacteria - E. Coli
Sample Types: Recombinant Protein
Applications: Bioassay -
Synthesis of Human Neutrophil Extracellular Traps Contributes to Angiopoietin-Mediated In Vitro Proinflammatory and Proangiogenic Activities
Authors: SS Lavoie, E Dumas, B Vulesevic, PE Neagoe, M White, MG Sirois
J. Immunol., 2018-04-23;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Recombinant Human Vimentin Binds to P-Selectin and Blocks Neutrophil Capture and Rolling on Platelets and Endothelium
Authors: FW Lam, Q Da, B Guillory, MA Cruz
J. Immunol., 2018-01-15;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Blocking -
Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity
Sci Rep, 2016-11-28;6(0):37953.
Applications: Bioassay -
Single Neutralizing Monoclonal Antibodies Targeting the VP1 GH Loop of Enterovirus 71 Inhibit both Virus Attachment and Internalization during Viral Entry.
Authors: Ku Z, Ye X, Shi J, Wang X, Liu Q, Huang Z
J Virol, 2015-09-23;89(23):12084-95.
Species: Human
Sample Types: Complex Sample Type
Applications: Bioassay -
TleA, a Tsh-like autotransporter identified in a human enterotoxigenic Escherichia coli strain.
Authors: Gutierrez D, Pardo M, Montero D, Onate A, Farfan M, Ruiz-Perez F, Del Canto F, Vidal R
Infect Immun, 2015-02-23;83(5):1893-903.
Species: Bacteria - E. Coli
Sample Types: Cell Culture Supernates
Applications: Bioassay -
Broad spectrum activity of a lectin-like bacterial serine protease family on human leukocytes.
Authors: Ayala-Lujan, Jorge Lu, Vijayakumar, Vidhya, Gong, Mei, Smith, Rachel, Santiago, Araceli, Ruiz-Perez, Fernando
PLoS ONE, 2014-09-24;9(9):e107920.
Species: Bacteria, Human
Sample Types: Protein, Whole Cells
Applications: Enzyme Assay, Enzyme Assay Substrate -
Heterotropic modulation of selectin affinity by allosteric antibodies affects leukocyte rolling.
Authors: Riese S, Kuehne C, Tedder T, Hallmann R, Hohenester E, Buscher K
J Immunol, 2014-01-15;192(4):1862-9.
Species: Human
Sample Types: Protein, Whole Cells
Applications: Bioassay, Flow Cytometry -
Bromelain decreases neutrophil interactions with P-selectin, but not E-selectin, in vitro by proteolytic cleavage of P-selectin glycoprotein ligand-1.
Authors: Banks, Jessica, Herman, Christin, Bailey, Ryan C
PLoS ONE, 2013-11-11;8(11):e78988.
Applications: Bioassay -
Regulation of leukocyte recruitment by the long pentraxin PTX3.
Authors: Deban L, Russo RC, Sironi M, Moalli F, Scanziani M, Zambelli V, Cuccovillo I, Bastone A, Gobbi M, Valentino S, Doni A, Garlanda C, Danese S, Salvatori G, Sassano M, Evangelista V, Rossi B, Zenaro E, Constantin G, Laudanna C, Bottazzi B, Mantovani A
Nat. Immunol., 2010-03-07;11(4):328-34.
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay -
A versatile polyacrylamide gel electrophoresis based sulfotransferase assay.
Authors: Wu ZL, Ethen CM, Larson S, Prather B, Jiang W
BMC Biotechnol., 2010-02-10;10(0):11.
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay -
Crystal structures of the staphylococcal toxin SSL5 in complex with sialyl Lewis X reveal a conserved binding site that shares common features with viral and bacterial sialic acid binding proteins.
Authors: Baker HM, Basu I, Chung MC, Caradoc-Davies T, Fraser JD, Baker EN
J. Mol. Biol., 2007-10-10;374(5):1298-308.
Applications: Western Blot -
Kinetic analysis of heparin and glucan sulfates binding to P-selectin and its impact on the general understanding of selectin inhibition.
Authors: Simonis D, Fritzsche J, Alban S, Bendas G
Biochemistry, 2007-04-26;46(20):6156-64.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance
FAQs
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Does the CHO-derived rhPSGL-1/CD162, Catalog # 3345-PS, have O-glycosylation and the Sialyl-LewisX modification?
Yes. We use proprietary methods to produce a functional rhPSGL-1 protein with O-glycosylation and the Sialyl-Lewis X Modification.
Reviews for Recombinant Human PSGL-1/CD162 Fc Chimera Protein, CF
Average Rating: 4 (Based on 1 Review)
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Reason for Rating: Good binding for protein-protein interaction assays
