Recombinant Human P-Selectin/CD62P Protein, CF Summary
Product Specifications
Trp42-Ala771
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
ADP3
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol. |
| Reconstitution | Reconstitute at 1 mg/mL in sterile, deionized water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: P-Selectin/CD62P
Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1).
P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L Selectin,P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling' of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.
ELISA techniques have shown that detectable levels of soluble P-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of P-Selectin may be elevated or lowered in subjects with a variety of pathological conditions.Citations for Recombinant Human P-Selectin/CD62P Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
17
Citations: Showing 1 - 10
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Single cell autofluorescence imaging reveals immediate metabolic shifts of neutrophils with activation across biological systems
Authors: Datta, R;Miskolci, V;Gallego-López, GM;Britt, E;Gillette, A;Kralovec, A;Giese, MA;Qian, T;Fan, J;Huttenlocher, A;Skala, MC;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Whole Cells
Applications: Immunocytochemistry -
P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression
Authors: E Yeini, P Ofek, S Pozzi, N Albeck, D Ben-Shusha, G Tiram, S Golan, R Kleiner, R Sheinin, S Israeli Da, S Reich-Zeli, R Grossman, Z Ram, H Brem, TM Hyde, P Magod, D Friedmann-, A Madi, R Satchi-Fai
Nature Communications, 2021-03-26;12(1):1912.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
L-Arginine Modulates Neonatal Leukocyte Recruitment in a Gestational Age-Dependent Manner
Authors: R Fitterer, T Lajqi, SA Kranig, M Braun, N Theissig, N Kuss, J Pöschl, D Frommhold, H Hudalla
J Clin Med, 2020-08-27;9(9):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Platelets Fuel the Inflammasome Activation of Innate Immune Cells
Authors: V Rolfes, LS Ribeiro, I Hawwari, L Böttcher, N Rosero, S Maasewerd, MLS Santos, T Próchnicki, CMS Silva, CWS Wanderley, M Rothe, SV Schmidt, HJ Stunden, D Bertheloot, MN Rivas, CJ Fontes, LH Carvalho, FQ Cunha, E Latz, M Arditi, BS Franklin
Cell Rep, 2020-05-12;31(6):107615.
Species: Human
Sample Types:
Applications: Cell Culture -
Glycosaminoglycans as Tools to Decipher the Platelet Tumor Cell Interaction: A Focus on P-Selectin
Authors: S Schwarz, LM Gockel, A Naggi, U Barash, M Gobec, G Bendas, M Schlesinge
Molecules, 2020-02-26;25(5):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The P-selectin and PSGL-1 axis accelerates atherosclerosis via activation of dendritic cells by the TLR4 signaling pathway
Authors: Z Ye, L Zhong, S Zhu, Y Wang, J Zheng, S Wang, J Zhang, R Huang
Cell Death Dis, 2019-07-01;10(7):507.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Neutrophil activation and NETosis are the major drivers of thrombosis in heparin-induced thrombocytopenia
Authors: J Perdomo, HHL Leung, Z Ahmadi, F Yan, JJH Chong, FH Passam, BH Chong
Nat Commun, 2019-03-21;10(1):1322.
Species: Human
Sample Types: Whole Blood
Applications: Bioassay -
L-selectin expression is regulated by CXCL8-induced reactive oxygen species produced during human neutrophil rolling
Authors: MJ Domínguez-, E Armas-Gonz, A Herrera-Ga, M Arce-Franc, M Feria, M Vicente-Ma, A Martínez-R, F Sánchez-Ma, F Díaz-Gonzá
Eur. J. Immunol., 2018-12-05;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity
Sci Rep, 2016-11-28;6(0):37953.
Applications: ELISA Developmet -
Platelets Guide Leukocytes to Their Sites of Extravasation
PLoS Biol, 2016-05-06;14(5):e1002459.
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay -
Endocarditis pathogen promotes vegetation formation by inducing intravascular neutrophil extracellular traps through activated platelets.
Authors: Jung C, Yeh C, Hsu R, Lee C, Shun C, Chia J
Circulation, 2014-12-19;131(6):571-81.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Saikosaponin D isolated from Bupleurum falcatum inhibits selectin-mediated cell adhesion.
Authors: Jang M, Kim Y, Bae E, Oh T, Choi H, Lee J, Oh H, Lee S
Molecules, 2014-12-04;19(12):20340-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
VLA-4 blockade promotes differential routes into human CNS involving PSGL-1 rolling of T cells and MCAM-adhesion of TH17 cells.
Authors: Schneider-Hohendorf T, Rossaint J, Mohan H, Boning D, Breuer J, Kuhlmann T, Gross C, Flanagan K, Sorokin L, Vestweber D, Zarbock A, Schwab N, Wiendl H
J Exp Med, 2014-08-18;211(9):1833-46.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
RAGE and ICAM-1 cooperate in mediating leukocyte recruitment during acute inflammation in vivo.
Authors: Frommhold D, Kamphues A, Hepper I, Pruenster M, Lukic IK, Socher I, Zablotskaya V, Buschmann K, Lange-Sperandio B, Schymeinsky J, Ryschich E, Poeschl J, Kupatt C, Nawroth PP, Moser M, Walzog B, Bierhaus A, Sperandio M
Blood, 2010-04-20;116(5):841-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Regulation of leukocyte recruitment by the long pentraxin PTX3.
Authors: Deban L, Russo RC, Sironi M, Moalli F, Scanziani M, Zambelli V, Cuccovillo I, Bastone A, Gobbi M, Valentino S, Doni A, Garlanda C, Danese S, Salvatori G, Sassano M, Evangelista V, Rossi B, Zenaro E, Constantin G, Laudanna C, Bottazzi B, Mantovani A
Nat. Immunol., 2010-03-07;11(4):328-34.
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay -
Capture and enrichment of CD34-positive haematopoietic stem and progenitor cells from blood circulation using P-selectin in an implantable device.
Authors: Wojciechowski JC, Narasipura SD, Charles N, Mickelsen D, Rana K, Blair ML, King MR
Br. J. Haematol., 2008-01-22;140(6):673-81.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
Staphylococcal superantigen-like 5 binds PSGL-1 and inhibits P-selectin-mediated neutrophil rolling.
Authors: Bestebroer J, Poppelier MJ, Ulfman LH, Lenting PJ, Denis CV, van Kessel KP, van Strijp JA, de Haas CJ
Blood, 2007-04-01;109(7):2936-43.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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