Recombinant Human OSMR beta Protein, CF Summary
Product Specifications
Glu28-Ser739
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
4389-OR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: OSMR beta
OSM R beta is a 150 - 180 kDa member of the IL-6 receptor family. It associates with gp130 to form the type II OSM receptor that is responsive to OSM. The gp130 subunit is shared by other IL-6 family cytokine receptors (1, 2, 3, 4), and OSM R beta associates with gp130-like receptor (GPL) to form a receptor complex responsive to IL-31 (5, 6). The human OSM R beta cDNA encodes a 979 amino acid (aa) precursor that includes a 27 aa signal sequence, a 712 aa extracellular domain (ECD), a 22 aa transmembrane segment, and a 218 aa cytoplasmic domain. The ECD contains one partial and one complete hematopoietin domain, an Ig-like domain, and three fibronectin type-III domains. The cytoplasmic domain contains box1, 2, and 3 motifs (7). Within the ECD, human OSM R beta shares 55%, 58%, 61%, and 72% aa sequence identity with mouse, rat, bovine, and canine OSM R beta, respectively. It also shares 31% aa sequence identity with human LIF R, but less than 20% aa sequence identity with human CNTF R alpha, G-CSF R, IL-6 R, IL-11 R alpha, and TCCR. OSM R beta does not bind cytokines directly, but increases the affinity of gp130 for OSM, and GPL for IL-31 (7, 8). OSM R beta, gp130, and GPL each initiate signaling events following ligand stimulation (9, 10). Jak/STAT and MAPK pathways are activated by OSM R beta -containing receptors (9, 11, 12, 13), including STAT5b and SHC which are not activated by other IL-6 family receptors (10, 13). In mice, the loss of OSM R beta expression blocks erythroid progenitor development in bone marrow, and dramatically reduces the number of circulating platelets and erythrocytes (14). The type II OSM receptor is the only IL-6 family receptor that promotes osteoblast differentiation in calvaria cell cultures (15).
- Chen, S.-H. and E.N. Benveniste (2004) Cytokine Growth Factor Rev. 15:379.
- Heinrich, P.C. et al. (2003) Biochem. J. 374:1.
- Tanaka, M. and A. Miyajima (2003) Rev. Physiol. Biochem. Pharmacol. 149:39.
- Gearing, D.P. et al. (1992) Science 255:1434.
- Dillon, S.R. et al. (2004) Nat. Immunol. 5:752.
- Diveu, C. et al. (2003) J. Biol. Chem. 278:49850.
- Mosley, B. et al. (1996) J. Biol. Chem. 271:32635.
- Diveu, C. et al. (2004) Eur. Cytokine Netw. 15:291.
- Dreuw, A. et al. (2004) J. Biol. Chem. 279:36112.
- Wang, Y. et al. (2000) J. Biol. Chem. 275:25273.
- Hermanns, H.M. et al. (2000) J. Biol. Chem. 275:40742.
- Kuropatwinski, K.K. et al. (1997) J. Biol. Chem. 272:15135.
- Auguste, P. et al. (1997) J. Biol. Chem. 272:15760.
- Tanaka, M. et al. (2003) Blood 102:3154.
- Malaval, L. et al. (2005) J. Cell. Physiol. 204:585.
Citations for Recombinant Human OSMR beta Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Engineered Interleukin-6-derived cytokines recruit artificial receptor complexes and disclose CNTF signaling via the OSMR
Authors: Rafii, P;Cruz, PR;Ettich, J;Seibel, C;Padrini, G;Wittich, C;Lang, A;Petzsch, P;Köhrer, K;Moll, JM;Floss, DM;Scheller, J;
The Journal of biological chemistry
Species: N/A
Sample Types: Recombinant Protein
Applications: Immunoprecipitation -
A unique loop structure in oncostatin M determines binding affinity toward oncostatin M receptor and leukemia inhibitory factor receptor.
Authors: Chollangi, Srinivas, Mather, Timothy, Rodgers, Karla K, Ash, John D
J Biol Chem, 2012-07-24;287(39):32848-59.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA (Capture), Surface Plasmon Resonance
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