Recombinant Human OSCAR Fc Chimera Protein, CF Summary
Product Specifications
| Human OSCAR (Asp19-Asn229) Accession # NP_573399 | IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9955-OS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: |
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Scientific Data
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When Bovine Collagen I is coated at 10 µg/mL, Recombinant Human OSCAR Fc Chimera binds with an ED50 of 1-8 ng/mL.
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2 μg/lane of Recombinant Human OSCAR was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 61-70 kDa and 120-140 kDa, respectively.
Reconstitution Calculator
Background: OSCAR
OSCAR (Osteoclast-associated immunoglobulin-like receptor), also known as PIgR-3 (polymeric immunoglobulin-like receptor), is a type I transmembrane protein of the leukocyte receptor complex (LRC) family (1). The mature human OSCAR includes an extracellular domain (ECD) with two Ig domains homology to PIR family proteins, a transmembrane region, and a cytoplasmic domain. The ECD of human OSCAR shares 77% and 72% amino acid sequence identity with the ECD of mouse and rat OSCAR, respectively. OSCAR is specifically expressed by pre-osteoclasts and signals via FcR gamma (2). OSCAR co-stimulates one of the major FcR gamma -associated pathways required for osteoclastogenesis in vivo (2). Collagen type I and collagen type II serve as ligands for OSCAR (3). OSCAR binds to specific motifs within fibrillary collagens in the extracellular matrix (ECM) (2). OSCAR promoted osteoclastogenesis in vivo, and its binding to the collagen motif led to signaling that increased numbers of osteoclasts in culture (2). OSCAR contains two immunoglobulin-like domains, D1 and D2. Direct binding assays on OSCAR molecules showed that D2, but not D1, is critical for collagen binding (4). OSCAR may contribute to the pathogenesis and severity of a number of diseases including osteoporosis, atherosclerosis, chronic obstructive pulmonary disease and rheumatoid arthritis (4).
- Kim N. el al. (2002) J. Exp. Med. 195:201.
- Barrow, A.D. et al. (2011) J. Clin. Invest. 121:3505.
- Shultz, H.S. et al. (2016) Eur. J. Immunol. 46:952.
- Zhou, L. et al. (2016) Blood 127:529.
Citation for Recombinant Human OSCAR Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Collagen-like Osteoclast-Associated Receptor (OSCAR)-Binding Motifs Show a Co-Stimulatory Effect on Osteoclastogenesis in a Peptide Hydrogel System
Authors: Vitale, M;Ligorio, C;Richardson, SM;Hoyland, JA;Bella, J;
International journal of molecular sciences
Applications: Surface Plasmon Resonance (SPR)
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