Recombinant Human Oncostatin M (HEK-293-expressed) Protein
Recombinant Human Oncostatin M (HEK-293-expressed) Protein Summary
Product Specifications
Ala26-Arg221
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8475-OM
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
8475-OM/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Oncostatin M/OSM
Oncostatin M (OSM) is an approximately 30 KDa secreted cytokine belonging to the Interleukin-6 family. Like other members of the IL-6 family such as IL-11, CNTF, and Cardiotrophin-1, OSM plays crucial roles in inflammation, neuroprotection, hematopoiesis, metabolism and development (1). The human OSM cDNA encodes a 252 amino acid (aa) prepro protein with a signal peptide and a hydrophilic Cterminal domain that are proteolytically processed to generate the 195 aa mature form (2). Although both mature and pro-OSM are equally active in radioreceptor assays, the mature OSM is 5 to 60 fold more active in growth inhibition assays (3). Thus, proteolytic processing of the pro-OSM peptide may be important in regulating the in vivo activities of OSM (3). Cytokines of the IL-6 family share a common receptor subunit, gp130, which allows intracellular signal transduction. In contrast to other cytokines, OSM binds gp130 with very low affinity and has little to no biological activity unless a second receptor chain is recruited (4). The OSM-gp130 complex recruits either LIF R alpha, or OSM R beta to form the OSM receptor type I or OSM receptor type II respectively (5). Among multiples roles, OSM has been reported to modulate tumor cell and epithelial cell growth (6, 7). An axis involving OSM, CD40 Ligand and OSM receptor described as an antiviral and immunostimulatory system is disrupted in patients liver showing hepatitis C infection (8). Human OSM shares 45% aa sequence homology with mouse and rat OSM.
- Richards, C.D. (2013) ISRN Inflamm. 2013:512103.
- Malik, N. et al. (1989) Mol. Cel. Biol. 9:2847.
- Linsley, P.S. et al. (1990) Mol. Cel. Biol. 10:1882.
- Liu, J. et al. (1994) Cytokine 6:272.
- Mosley, B. et al. (1996) J. Biol. Chem. 271:32635.
- Grant, S.L. et al. (2001) Growth Factors 19:153.
- Chollangi, S. et al. (2012) J. Biol. Chem. 287:32848.
- Larrea, E. et al. (2014) J. Hepatol. 60:482.
Citations for Recombinant Human Oncostatin M (HEK-293-expressed) Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
2
Citations: Showing 1 - 2
Filter your results:
Filter by:
-
Mast cells disrupt the function of the esophageal epithelial barrier
Authors: Kleuskens, MTA;Bek, MK;Al Halabi, Y;Blokhuis, BRJ;Diks, MAP;Haasnoot, ML;Garssen, J;Bredenoord, AJ;C A M van Esch, B;Redegeld, FA;
Mucosal immunology
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
A nonalcoholic fatty liver disease model in human induced pluripotent stem cell-derived hepatocytes, created by endoplasmic reticulum stress-induced steatosis
Authors: M Parafati, RJ Kirby, S Khorasaniz, F Rastinejad, S Malany
Dis Model Mech, 2018-09-25;11(9):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
-
What are the differences between Recombinant Human Oncostatin M Protein (Catalog # 295-OM) and Recombinant Human Oncostatin M (HEK293-expressed) Protein (Catalog # 8475-OM)?
Recombinant Human Oncostatin M Protein (Catalog # 295-OM) and Recombinant Human Oncostatin M (HEK293-expressed) Protein (Catalog # 8475-OM) share the same protein sequence, Ala26-Arg221 of Accession # P13725, but Catalog # 295-OM was expressed in E. coli and Catalog # 8475-OM was expressed in HEK293 cells. They are offered in formulations with or without BSA as a carrier protein.
Both proteins are routinely tested and have the same activity range in our established QC bioassay: "Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells." The ED50 for this effect is 0.05-0.3 ng/mL. Catalog # 8475-OM is produced in a mammalian expression system so we would expect it to be glycosylated. Catalog # 295-OM is produced in E coli and is not glycosylated.
If endotoxin levels are of concern for your application, we would recommend selecting the Catalog # 295-OM as it has an endotoxin threshold of <0.01 EU per 1 μg of protein. Catalog # 8475-OM has a higher endotoxin threshold of <0.10 EU per 1 μg of the protein. That said, if it is important to use a mammalian expressed protein like Catalog # 8475-OM in your assay system, and you require endortoxin lower than <0.10 EU/1 μg of the protein please consult technical service to see if custom testing is an option. We also recommend reviewing the references under the Citations tab on the respective product pages to check the types of applications in which these two proteins have been evaluated.
-
Is Recombinant Human Oncostatin M (HEK-293-expressed) Protein (Catalog # 8475-OM) considered the pre-OSM peptide or mature protein?
Recombinant Human Oncostatin M (HEK-293-expressed) Protein (Catalog # 8475-OM) is comprised of amino acids Ala26-Arg221 of Accession # P13725. This region is the 196 residue mature form of OSM.
Reviews for Recombinant Human Oncostatin M (HEK-293-expressed) Protein
Average Rating: 4 (Based on 1 Review)
Have you used Recombinant Human Oncostatin M (HEK-293-expressed) Protein?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
