Recombinant Human NKp65/KLRF2 Fc Chimera Protein, CF Summary
Product Specifications
| MD | Human IgG1 (Pro100-Lys330) | IEGR | Human NKp65/KLRF2 (Asp52-Val207) Accession # D3W0D1.1 |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10371-NK
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
View Larger
When Recombinant Human NKp65/KLRF2 Fc Chimera (Catalog # 10371-NK) is immobilized at 0.1 µg/mL, 100 µL/well, the concentration for Recombinant Human CLEC-2A (Catalog # 8435-CL) that produces 50% of the optimal binding response is approximately 0.5-3 ng/mL.
View Larger
2 μg/lane of Recombinant Human NKp65/KLRF2 Fc Chimera (Catalog # 10371-NK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-65 kDa and 100-130 kDa, respectively.
Reconstitution Calculator
Background: NKp65/KLRF2
Killer cell lectin-like receptor subfamily F member 2, also known as KLRF2 and NKp65, is a C-type lectin-like receptor and a member of the NKRP1 (NK receptor protein) subfamily (1-3). KLRF2, along with the related KLRF1, is an activating receptor for the C-type-lectin-like-2 (CLEC2) family of ligands (1-3). KLRF2 is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic domain, a single transmembrane region and an extracellular domain (ECD) consisting of 26-residue stalk and 130-residue C-type lectin-like domain (CTLD) (1, 3). Unlike other members of the NKRP1 family such as KLRF1, KLRF2 is a non-disulfide-linked homodimer (1, 2). While sharing subfamily-specific traits classifying them as members of the NKRP1 subfamily, there does not appear to be a direct homolog of KLRF2 in mouse (2). KLRF2 is minimally expressed on peripheral blood NK cells and its ligand, Keratinocyte-associated C-type lectin (KACL), is restricted to human keratinocytes (1-3). This expression of KACL indicates potential implications in skin-affecting diseases such as psoriasis and wound healing (1). The signaling pathway of KLRF2 is distinct from most NK receptors because it does not utilize immunoreceptor tyrosine-based activating motif (ITAM)-containing signaling adaptors, but rather uses an activating signaling motif with only one tyrosine module referred to as hemITAM (1, 3).
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human NKp65/KLRF2 Fc Chimera Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human NKp65/KLRF2 Fc Chimera Protein, CF and earn rewards!
Have you used Recombinant Human NKp65/KLRF2 Fc Chimera Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
