Home / NKG2A/CD159a / Recombinant Human NKG2A/CD159a Fc Chimera Protein, CF

Recombinant Human NKG2A/CD159a Fc Chimera Protein, CF

Catalog #: 10511-NK Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
10511-NK-050
Recombinant Human NKG2A/CD159a Fc Chimera Protein Binding Activity.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human NKG2A/CD159a Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD94 His-tag (Catalog # 9270-CD) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human NKG2A/CD159a Fc Chimera (Catalog # 10511-NK) binds with an ED50 of 2-16 µg/mL.
Source
Human embryonic kidney cell, HEK293-derived human NKG2A/CD159a protein
MDHuman IgG1
(Pro100-Lys330)		IEGRHuman NKG2A/CD159a
(Pro94-Leu233)
Accession # P26715.2
N-terminusC-terminus
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
43 kDa
SDS-PAGE
55-65 kDa, under reducing conditions

Product Datasheets

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10511-NK

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10511-NK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Recombinant Human CD94 His-tag (9270-CD) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human NKG2A/CD159a Fc Chimera (Catalog # 10511-NK) binds with an ED50 of 2-16 µg/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human NKG2A/CD159a Fc Chimera Protein (10511-NK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-65 kDa and 110-130 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NKG2A/CD159a

Killer cell lectin like receptor C1 (KLRC1), also known as NKG2A/NKG2B type II integral membrane protein or NK cell receptor A, is a member of the C-type lectin family (1). The NKG2 family is comprised of three unique receptor isoforms, NKG2A, NKG2C and NKG2E, which regulate both inhibitory and activating signals and are attractive targets for immune-checkpoint inhibition (1-3). Mature human NKG2A consists of a cytoplasmic domain containing two immunoreceptor tyrosine based inhibitory motifs (ITIM), a helical type II transmembrane domain, and an extracellular domain (ECD) with a C-type lectin motif (4). Within the mature ECD, human NKG2A shares 41% and 45% amino acid sequence identity with mouse and rat NKG2A, respectively. NKG2A is an inhibitory member of the NKG2 family and is expressed on CD56high natural killer (NK) cells, natural killer T (NKT) cells and a subset of CD8+ alpha beta T cells. NKG2A forms a disulfide linked, dimeric complex with another C-type lectin, CD94 (KLRD1), and functions by binding with HLA-E, a non-classical MHC I molecule (1-4). The increased expression of NKG2A in tumor‑infiltrating NK cells is also emerging as a contributor in determining the poor prognosis of hepatocellular, lung or other carcinomas and in some cases may be a predictive factor of tumor metastasis (5). NKG2A immune checkpoint blockade in combination with PD-1/PD-L1 blockade or therapeutic cancer vaccines may hold promise in the treatment or multiple myeloma (MM) and various other solid and blood tumors or may improve the clinical outcome of patients who have undergone allogeneic hematopoietic stem cell transplantation (5-9).

References
  1. Haanen, J. and Cerundolo, V. (2018) Cell. 175:1720.
  2. Ceelan, B.C. and Antonia, S.J. (2019) Antonia Nature Reviews Clinical Oncology 16:277.
  3. Montfoort, N. et al. (2018) Cell. 175:1744.
  4. Brooks, A. et al. (1997) J. Exp. Med. 185:795.
  5. Zaghi, E. et al. (2019) J Leukoc Biol. 105:1243.
  6. van Hall, T. et al. (2019) J Immunother Cancer. 7:263.
  7. André, P. et al. (2019) Cell. 175:1731.
  8. Mahaweni, N.M. et al. (2018) Front Immunol. 9:2848.
  9. Borst, L. et al. (2020) doi: 10.1158/1078-0432.CCR-19-2095.
Long Name
Natural Killer G2A
Entrez Gene IDs
3821 (Human); 16641 (Mouse); 102121106 (Cynomolgus Monkey)
Alternate Names
CD159 antigen-like family member A; CD159a antigen; CD159a; C-lectin type II protein; killer cell lectin-like receptor subfamily C, member 1; Klrc1; MGC13374; natural killer cell lectin; natural killer group protein 2; NK cell receptor A; NKG2; NKG2-1/B activating NK receptor; NKG2A; NKG2-A; NKG2-A/B type II integral membrane protein; NKG2-A/B-activating NK receptor; NKG2-A/NKG2-B type II integral membrane protein; NKG2AMGC59791; NKG2-B

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