Recombinant Human NFATC2 Protein, CF

Catalog #: 9716-NA Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9716-NA-050

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Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Human NFATC2 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

E. coli-derived human NFATC2 protein
His575-Pro679, with an N-terminal Met and a C-terminal 6-His tag

Accession #

Q13469-1

N-terminal Sequence
Analysis

Met

Predicted Molecular Mass

13 kDa

SDS-PAGE

16 kDa, reducing conditions

Product Datasheets

9716-NA

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9716-NA

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 500 μg/mL in 4 mM HCl..
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: NFATC2

Nuclear factor of activated T cells, cystoplamic 2 (NFATC2), also known as T cell transcription factor NFAT1, is a member of the calcium-regulated family of transcription factors that regulate gene expression in lymphocytes and control cardiac valve formation (1, 2). The NFAT family is comprised of four members, NFATC1-C4. Human NFATC2 is a 925 aa transcription factor containing several key regions including a calcineurin-binding site, a trans-activation domain, and nuclear localization and export signals. The first three members of this family are thought to be immune-specific forms of NFAT, while NFATC4 has functions in the regulation of cardiac hypertrophy and hippocampal neuronal signaling (3, 4). In resting T cells, the NFAT proteins are present in the cytoplasm in their phosphorylated form. Upon elevation of intracellular calcium concentration and activation of the phosphatase calcineurin, the NFATC proteins are dephosphorylated and translocated into the nucleus where they regulate the transcription of genes for a number of cytokines and surface molecules (5, 6).

References

Rengarajan, J. et al. (2002) Nat. Immunol. 3:48.
Baksh, S. et al. (2002) Mol. Cell. 10:1071.
Molkentin, J.D. et al. (1998) Cell. 93:215.
Graef, I.A. et al. (1999) Nature. 401:703.
Bopp, T. et al. (2005) J. Exp. Med. 201:181.
Ranger, A.M. et al. (2000) J. Exp. Med. 191:9.

Long Name

Nuclear factor of activated T-cells, cytoplasmic 2

Entrez Gene IDs

4773 (Human)

Alternate Names

NFAT pre-existing subunit; NFAT transcription complex, preexisting component; NFAT1; NFAT1nuclear factor of activated T-cells, cytoplasmic 2; NFATC2; NF-ATc2; NFATP; NF-ATp; NFATPnuclear factor of activated T-cells, preexisting component; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2; preexisting nuclear factor of activated T-cells 2; T cell transcription factor NFAT1; T-cell transcription factor NFAT1