Recombinant Human/Mouse/Rat Irisin Protein Summary
Product Specifications
Asp32-Glu143, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8880-IR
| Formulation | Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and TCEP with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
8880-IR/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and TCEP. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
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3T3-L1 preadipocytes were incubated with 1 µg/ml of Recombinant Human Irisin for 15 minutes. The cells were lysed and Western blots were performed with anti-phospho p38 MAPK and total p38 MAPK antibodies. Experiments are shown in duplicate.
Reconstitution Calculator
Background: Irisin/FNDC5
Irisin (also known as FNDC5) is a 12 kDa glycosylated polypeptide hormone that regulates energy metabolism, stem cell differentiation, and neuronal development (1, 2). Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation (2-5). Mature human, mouse, and rat Irisin share 100% sequence identity. Expression of Irisin is induced in skeletal muscle and subcutaneous adipose tissue during and shortly after exercise (2, 6). Irisin induces expression of peroxisome proliferator-activated receptor gamma co-activator 1 alpha (PGC1 alpha ) and uncoupling protein-1 (UCP1), mitochondrial-associated metabolic proteins (7, 8). Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue. In mice, expression of Irisin has been shown to regulate obesity and diabetes (1, 2). A similar function in humans is suggested (9). Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts (10-14).
Citations for Recombinant Human/Mouse/Rat Irisin Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Irisin mitigates rheumatoid arthritis by suppressing mitochondrial fission via inhibiting YAP-Drp1 signaling pathway
Authors: Yu, Y;Ma, M;Li, C;Dang, Q;Lei, H;Wang, G;Su, J;Li, Y;
International immunopharmacology
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: In Vivo, Bioassay -
Exercise-induced FNDC5/irisin protects nucleus pulposus cells against senescence and apoptosis by activating autophagy
Authors: W Zhou, Y Shi, H Wang, L Chen, C Yu, X Zhang, L Yang, X Zhang, A Wu
Oncogene, 2022-07-26;54(7):1038-1048.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay
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