Recombinant Human MOG Protein, CF Summary
Product Specifications
Gly30-Gly154, with a substitution at Asp131Ala and a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8535-MO
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: MOG
Myelin oligodendrocyte glycoprotein (MOG) is 28 kDa single-pass transmembrane glycoprotein that is a member of the Ig superfamily (1, 2). Human MOG is synthesized with a 29 amino acid (aa) signal sequence, a 125 aa extracellular domain (ECD) containing an Ig-like domain, a 21 aa transmembrane domain, and a 72 aa cytosolic fragment featuring a hydrophobic domain that associates with the cytoplasmic face of the plasma membrane. The ECD of mature human MOG shares 90% aa sequence identity with the ECD of both mouse and rat MOG. Unlike mouse and rat, human MOG has seven splice variants, including a soluble ECD isoform and multiple isoforms that have a truncated cytoplasmic domain (3, 4). Dimerization of MOG occurs via the extracellular Ig-like domain (5-8). MOG is expressed exclusively by oligodendrocytes in the central nervous system (CNS) and is localized to the outer layer of the myelin sheath as well as in the oligodendrocyte plasma membrane (9). MOG expression in the brain can be used as a temporal biomarker for myelin development. MOG is an important antigenic target for autoimmune diseases that mediate demyelination in the CNS (10). In vivo administration of exogenous MOG protein or peptide induces experimental autoimmune encephalomyelitis (EAE) in multiple animal species (11, 12). EAE is used as an animal model for multiple sclerosis and related CNS demyelinating diseases. MOG is thought to function as an adhesion molecule as well as a mediator of immune activation in the CNS (9, 13).
- Johns, T.G. and C.C. Bernard (1999) J. Neurochem. 72:1.
- Pham-Dinh, D. et al. (1993) Proc. Natl. Acad. Sci. U S A 90:7990.
- Hilton, A.A. et al. (1995) J. Neurochem. 65:309.
- Slavin, A.J. et al. (1997) Dev. Neurosci. 19:69.
- Abo, S. et al. (1993) Biochem. Mol. Biol. Int. 30:945.
- Amiguet, P. et al. (1992) J. Neurochem. 58:1676.
- Bettadapura, J. et al. (1998) J. Neurochem. 70:1593.
- Clements, C.S. et al. (2003) Proc. Natl. Acad. Sci. U S A 100:11059.
- Reindl, M. et al. (2013) Nat. Rev. Neurol. 9:455.
- von Budingen, H.C. et al. (2004) Eur. J. Immunol. 34:2072.
- Rangachari, M. and V.K. Kuchroo (2013) J. Autoimmun. 45:31.
- Tompkins, S.M. et al. (2002) J. Immunol. 168:4173.
- Garcia-Vallejo, J.J. et al. (2014) J. Exp. Med. 211:1465.
Citation for Recombinant Human MOG Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Remibrutinib (LOU064) inhibits neuroinflammation driven by B cells and myeloid cells in preclinical models of multiple sclerosis
Authors: Nuesslein-Hildesheim, B;Ferrero, E;Schmid, C;Huck, C;Smith, P;Tisserand, S;Rubert, J;Bornancin, F;Eichlisberger, D;Cenni, B;
Journal of neuroinflammation
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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