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Recombinant Human Mindin His-tag Protein, CF

Catalog #: 11539-SP Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
11539-SP-050
Recombinant Human Mindin His-tag Protein Binding Activity.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Mindin His-tag Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind fluorescein-conjugated E. coli Bioparticles. The ED50 for this effect is 0.200-2.40 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Mindin protein
Glu32-Val331, with a C-terminal 6-His tag
Accession #
AAH02707.1
N-terminal Sequence
Analysis
Glu 32
Predicted Molecular Mass
34 kDa
SDS-PAGE
38-44 kDa, under reducing conditions

Product Datasheets

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11539-SP

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11539-SP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. 
Reconstitution Reconstitute lyophilized material at 250 μg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

Recombinant Human Mindin His-tag Protein (Catalog # 11539-SP) binds to fluorescein-conjugated E. coli Bioparticles. The ED50 for this effect is 0.200‑2.40 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Mindin

Mindin, also known as Spondin-2 and DIL-1, is a member of the Mindin F-Spondin family of evolutionarily conserved, secreted extracellular matrix proteins. It is composed of an N-terminal F-spondin domain, which mediates integrin binding, and a C-terminal thrombospondin type I repeat, which binds to bacterial lipopolysaccharide (1).  The mature human protein shares 88% aa sequence identity with the mouse orthologs (2). Mindin is expressed in a variety of tissues in mice with the highest expression levels being detected in lung, spleen, heart, and lymph nodes (2). Mindin is essential for initiating innate and adaptive immune responses. It has been shown to be a pattern recognition receptor for bacterial pathogens and function as an opsonin for macrophage phagocytosis of bacteria (1, 2). Mindin also binds directly to influenza virus particles and is necessary for macrophage-dependent clearance of influenza viruses from the nasal cavity (3). In addition, Mindin serves as a ligand for leukocyte integrins (1). It is critical for T cell priming and recruitment of macrophages and neutrophils to sites of inflammation (4, 5). Mindin also regulates trafficking of eosinophils and granulocytes into the airspace and plays a role in the development of allergic airways disease (6, 7). Outside the immune system, Mindin has been shown to promote adhesion and outgrowth of hippocampal embryonic neurons, and to be an important mediator of brain ischemic injury and cardiac hypertrophy (8-11). Furthermore, it has been suggested that Mindin can serve as a marker for prostate and ovarian cancer, as well as diabetic nephropathy (12-14).

References
  1. Li, Y. et al. (2009) EMBO J. 28:286.
  2. He, Y.W. et al. (2004) Nat. Immunol. 5:88.
  3. Jia, W. et al. (2008) J. Immunol. 180:6255.
  4. Li, H. et al. (2006) EMBO J. 25:4097.
  5. Jia, W. et al. (2005) Blood 106:3854.
  6. Li, Z. et al. (2009) J. Leukoc. Biol. 85:124.
  7. Tighe, R.M. et al. (2011) J. Allergy Ther. 2011 (Suppl. 1). pii: 001.
  8. Feinstein, Y. et al. (1999) Development 126:3637.
  9. Yan, L. et al. (2011) Cardiovasc. Res. 92:85.
  10. Bian, Z.Y. et al. (2012) J. Mol. Med. (Berl.) 90:895.
  11. Wang, L. et al. (2013) Exp. Neurol. 247:506.
  12. Simon, I. et al. (2007) Gynecol. Oncol. 106:112.
  13. Murakoshi, M. et al. (2011) Exp. Diabetes Res. 2011:486305.
  14. Lucarelli, G. et al. (2013) J. Urol. 190:2271.
Entrez Gene IDs
10417 (Human); 100689 (Mouse)
Alternate Names
Differentially expressed in cancerous and non-cancerous lung cells 1; DIL1; DIL-1; DIL1FLJ34460; DKFZp686G21139; FLJ16313; FLJ22401; Mindin; M-SPONDIN; RG-1; SPON2; Spondin 2; spondin 2, extracellular matrix protein; spondin-2

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