Recombinant Human MICB His-tag Protein, CF Summary
Product Specifications
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10431-MB
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Scientific Data
View Larger
When Recombinant Human NKG2D/CD314 Fc Chimera (Catalog # 1299-NK) is immobilized at 0.125 µg/mL, 100 µL/mL, the concentration of Recombinant Human MICB His-tag (Catalog # 10431-MB) produces 50% of the optimal binding response is approximately 80-400 ng/mL.
View Larger
2 μg/lane of Recombinant Human MICB His-tag Protein (Catalog # 10431-MB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands 35-55 kDa.
Reconstitution Calculator
Background: MICB
Major Histocompatibility Complex Class I Chain-related Gene B (MICB) is a transmembrane glycoprotein that functions as a ligand for human Natural-Killer Group 2 Member D (NKG2D) (1). MICB, along with the highly related MICA, are members of the non-classical MHC class I family. Similar to classical MHC class I proteins, MICB contains three Ig-like domains ( alpha 1, alpha 2, and alpha 3) in the extracellular domain (ECD), a transmembrane segment, and a carboxy-terminal cytoplasmic tail (2). The genes encoding MICA and MICB are found within the MHC on the short arm of human chromosome 6 and occur in most mammalian species but are absent from mouse and rat (2-4). Despite structural similarities, MICA/B only share ~27% amino acid (aa) identity with human MHC class I proteins (3). Both MICA and MICB display a significant degree of polymorphism within the ECD and this enables them to be reconfigured and bind with NKG2D rather than binding with beta 2-microglobulin (4, 5). Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells (6, 7). MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response (2, 8-11). Inhibition of MICA/B shedding through targeted antibodies allows anti-tumor immunity to be retained (12).
- Huang, C. et al. (2018) Sci. Rep. 8:15821.
- Bahram, S. et al. (1994) Proc. Natl. Acad. Sci. USA 91:6259.
- Cosman, D. et al. (2001) Immunity 14:123.
- Groh, V. et al. (2001) Nature Immunol. 2:255.
- Stephens, H. (2001) Trends Immunol. 22:378.
- Bauer, S. et al. (1999) Science 285:727.
- Kawabata, Y. et al. (2000). Human Immunology. 61:624.
- Groh, V. et al. (2002) Nature 419:734.
- Steinle, A. et al. (2001) Immunogenetics 53:279.
- Pende, D. et al. (2002) Cancer Res. 62:6178.
- Salih, H. et al. (2003) Blood 102:1389.
- Ferrari de Andrade et al. (2018) Science 359:1537.
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human MICB His-tag Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human MICB His-tag Protein, CF and earn rewards!
Have you used Recombinant Human MICB His-tag Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
