Recombinant Human MDGA1 Protein, CF Summary
Product Specifications
Gln19-Gly931, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5055-MD
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: MDGA1
MDGA1 (MAM domain containing glycosylphosphatidylinositol anchor 1) is a 135‑140 kDa glycoprotein within the IgCAM superfamily of adhesion molecules (1‑3). MDGA1 is thought to mediate neuronal migration, neurite outgrowth, and cell-cell adhesion within the central and peripheral nervous system (1, 4‑8). Human MDGA1 precursor is a 955 amino acid (aa) protein that produces a 914 aa mature protein with six Ig-like domains, a fibronectin type III domain (aa 640-739), a MAM domain (aa 751-918), and a GPI anchor (aa 932) (1-3). Mature human MDGA1 shares 95%, 96%, and 98% aa sequence identity with mature mouse, rat, and canine MDGA1, respectively. Potential human variants of 973, 904 and 587 aa contain a 48 aa substitution for aa 936-955, a 58 aa substitution for aa 847-955, and a 51 aa substitution for aa 537-955, respectively. Human MDGA1 also shares 54% aa sequence identity with human MDGA2, and may also share some functional redundancy (1, 4). MDGA1 is mainly expressed on restricted populations of neurons in the central and peripheral nervous system, such as embryonic neurons destined for cortical layers 2/3, migrating basilar pontine neurons and D1 interneurons of the spinal cord (1, 5‑7). Deletion or down‑regulation of mouse MDGA1 slows radial migration of neurons, indicating a role for MDGA1 in radial migration of cortical neurons (4, 5). MAM and Ig-like domains are involved in heterophilic and homophilic adhesion (7, 8). MDGA1 expression is also reported on primary cells and cell lines from leukemias, lymphomas, and tumors of the lung, colon, uterus, stomach and breast (3).
- Litwack, E.D. et al. (2004) Mol. Cell. Neurosci. 25:263.
- Diaz-Lopez, A. et al. (2005) Exp. Cell Res. 307:91.
- De Juan, C. et al. (2002) Oncogene 21:3089.
- Ishikawa, T. et al. (2011) Dev. Dyn. 240:96.
- Takeuchi, A. and D.D.M. O'Leary (2006) J. Neurosci. 26:4460.
- Takeuchi, A. et al. (2007) Cereb Cortex. 17:1531.
- Fujimura, Y. et al. (2006) Brain Res. 1101:12.
- Diaz-Lopez, A. et al. (2011) Cancer Microenviron. 4:23.
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