Recombinant Human MAGP-2/MFAP5 Protein, CF Summary
Product Specifications
Ile22-Leu173 with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
4914-MG
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 400 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: MAGP-2/MFAP5
MAGP-2 (Microfibril-associated glycoprotein 2), also called MFAP5 (microfibril associated protein 5) is a secreted, cell-associated 25 kDa mammalian member of the MFAP family of proteins (1). Human MAGP-2 cDNA encodes 173 amino acids (aa) including a 21 aa signal sequence, an RDG motif (aa 30‑32; absent in MAGP-1) and a central cysteine‑rich binding domain (aa 84‑140) (1‑3). One potential splice variant shows a 10 aa deletion between aa 73‑82 that includes the only potential glycosylation site (4). Mature human MAGP-2 shares 80%, 82%, 82%, 84% and 85% aa identity with mouse, rat, bovine, porcine and canine MAGP-2, respectively. Both MAGP-1 and MAGP-2 bind and covalently crosslink fibrillins 1 and 2 through the binding domain, but MAGP-2 has more limited distribution and binds a more limited set of extracellular matrix proteins (1‑3). However, the MAGP-2 RGD motif binds to the integrin alpha v beta 3, allowing cell attachment to microfibrils (5). MAGP‑2 is thought to facilitate microfibril assembly and induce elastin fiber formation (1‑3, 6). Through its binding to EGF repeats, MAGP-2 mediates the metalloproteinase-dependent release of Jagged1 and the metalloproteinase-independent release of Notch extracellular domains (7, 8). In endothelial cells, MAGP-2 promotes angiogenic sprouting through inhibiting Notch signaling, binding integrin alpha v beta 3 (which then enhances VEGF signaling), and/or enhancing endothelial cell response to FGF basic and EGF (9, 10). In ovarian cancer, MAGP-2 expression is associated with cancer cell survival, increased microvessel density, and poor prognosis (11). Skin MAGP-2 expression is increased in human scleroderma and the tight skin (TSK) mouse; MAGP‑2 may contribute to abnormal collagen accumulation in these conditions by increasing the half-life of type I collagen (12, 13). Fibrillin-1 mutations within the region that binds MAGP-2 can be found in Marfan syndrome (6).
- Gibson, M.A. et al. (1996) J. Biol. Chem. 271:1096.
- Hanssen, E. et al. (2004) J. Biol. Chem. 279:29185.
- Segade, F. et al. (2002) J. Biol. Chem. 277:11050.
- Genbank protein Accession # AAQ18021.
- Gibson, M.A. et al. (1999) J. Biol. Chem. 274:13060.
- Lemaire, R. et al. (2007) J. Biol. Chem. 282:800.
- Nehring, L.C. et al. (2005) J. Biol. Chem. 280:20349.
- Miyamoto, A. et al. (2006) J. Biol. Chem. 281:10089.
- Albig, A.R. et al. (2008) Microvasc. Res. 76:7.
- Albig, A.R. et al. (2007) Angiogenesis 10:197.
- Mok, S.C. et al. (2009) Cancer Cell 16:521.
- Lemaire, R. et al. (2005) Arthritis Rheumatol. 52:1812.
- Ito, S. et al. (2006) Am.J. Respir. Cell Mol. Biol. 34:688.
Citation for Recombinant Human MAGP-2/MFAP5 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Microfibril-associated protein 5 and the regulation of skin scar formation
Authors: Han, C;Leonardo, TR;Romana-Souza, B;Shi, J;Keiser, S;Yuan, H;Altakriti, M;Ranzer, MJ;Ferri-Borgogno, S;Mok, SC;Koh, TJ;Hong, SJ;Chen, L;DiPietro, LA;
Scientific reports
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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