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Recombinant Human Lymphotoxin-alpha/TNF-beta Protein

Catalog #: 211-TBB
8 Citations 1 Review Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
211-TBB-010/CF
211-TBB-050/CF

With Carrier

Catalog # Availability Size / Price Qty
211-TBB-010
211-TBB-050
Recombinant Human Lymphotoxin-alpha/TNF-beta Protein Bioactivity
2 Images
Product Details
Citations (8)
FAQs
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Lymphotoxin-alpha/TNF-beta Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 4-20 pg/mL.
Source
E. coli-derived human Lymphotoxin-alpha/TNF-beta protein
Leu35-Leu205, with an N-terminal Met
Accession #
P01374
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
19 kDa
SDS-PAGE
17-19 kDa, reducing conditions

Product Datasheets

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211-TBB (with carrier)

Product Datasheet
COA
SDS

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211-TBB/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

211-TBB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

211-TBB/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human Lymphotoxin-alpha/TNF-beta Protein Bioactivity View Larger

Recombinant Human Lymphotoxin-alpha /TNF-beta (Catalog # 211-TBB) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 4-20 pg/mL.

SDS-PAGE Recombinant Human Lymphotoxin-alpha/TNF-beta Protein SDS-PAGE View Larger

1 µg/lane of Recombinant Human Lymphotoxin-alpha /TNF-beta was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 19 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lymphotoxin-alpha/TNF-beta

Lymphotoxin-alpha (LT-alpha ), also known as Tumor Necrosis Factor-beta (TNF-beta ), is a member of the TNF Superfamily. Human LT-alpha /TNF-beta is a 22 kDa protein that shares 73% amino acid sequence identity with mouse and rat LT-alpha /TNF-beta (1-3). Secreted LT-alpha forms homotrimers that bind and activate TNF RI/TNFRSF1A, TNF RII/TNFRSF1B, and HVEM/TNFRSF14 (4). LT-alpha /TNF-beta also forms heterotrimers with plasma membrane-localized LT-beta to bind and activate the LT-beta R/TNFRSF3 (4). In addition to its cytotoxic action on tumor cells, LT-alpha /TNF-beta mediates lymph node development, inflammation, and immune function (5-8). LT-alpha /TNF-beta is expressed in activated T- and B lymphocytes and contributes to autoimmune disease (9, 10).

References
  1. Nedwin, G.E. et al. (1985) J. Cell. Biochem. 29:171.
  2. Gray, P.W. et al. (1984) Nature 312:721.
  3. Kobayashi, Y. et al. (1986) J. Biochem. 100:727.
  4. Warzocha, K. et al. (1995) Eur. Cytokine Netw. 6:83.
  5. McCarthy, D.D. et al.(2006) Immunol. Res. 35:41.
  6. Drutskaya, M.S. et al. (2010) IUBMB Life 62:283.
  7. De Togni, P. et al. (1994) Science 264:703.
  8. Seleznik, G.M. et al. (2014) Cytokine Growth Factor Rev. 25:125.
  9. Mapara, M.Y. et al. (1994) Int. J. Cancer 58:248.
  10. Chiang, E.Y. et al. (2009) Nat. Med. 15:766.
Long Name
Tumor Necrosis Factor beta
Entrez Gene IDs
4049 (Human); 16992 (Mouse)
Alternate Names
LT; LTA; LT-alpha; lymphotoxin alpha (TNF superfamily, member 1); Lymphotoxin alpha; Lymphotoxinalpha; Lymphotoxin-alpha; tnfb; TNF-beta; TNFBlymphotoxin-alpha; TNFSF1; TNFSF1B; TNFSF1TNF-beta; tumor necrosis factor beta; Tumor necrosis factor ligand superfamily member 1

Citations for Recombinant Human Lymphotoxin-alpha/TNF-beta Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells
    Authors: M Fellermann, C Huchler, L Fechter, T Kolb, F Wondany, D Mayer, J Michaelis, S Stenger, K Mellert, P Möller, TFE Barth, S Fischer, H Barth
    Toxins (Basel), 2020-09-01;12(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. EphA2 contributes to disruption of the blood-brain barrier in cerebral malaria
    Authors: TK Darling, PN Mimche, C Bray, B Umaru, LM Brady, C Stone, CE Eboumbou M, TE Lane, LS Ayong, TJ Lamb
    PLoS Pathog., 2020-01-30;16(1):e1008261.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. BTKCys481Serdrives ibrutinib resistance via ERK1/2, and protects BTKWild-TypeMYD88 mutated cells by a paracrine mechanism
    Authors: JG Chen, X Liu, M Munshi, L Xu, N Tsakmaklis, MG Demos, A Kofides, ML Guerrera, GG Chan, CJ Patterson, KE Meid, J Gustine, T Dubeau, P Severns, JJ Castillo, ZR Hunter, J Wang, SJ Buhrlage, NS Gray, SP Treon, G Yang
    Blood, 2018-03-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Dendritic cell regulation of NK-cell responses involves lymphotoxin-alpha, IL-12, and TGF-beta.
    Authors: Sarhan D, Palma M, Mao Y, Adamson L, Kiessling R, Mellstedt H, Osterborg A, Lundqvist A
    Eur J Immunol, 2015-04-17;45(6):1783-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Generation and characterization of small single domain antibodies inhibiting human tumor necrosis factor receptor 1.
    Authors: Steeland S, Puimege L, Vandenbroucke R, Van Hauwermeiren F, Haustraete J, Devoogdt N, Hulpiau P, Leroux-Roels G, Laukens D, Meuleman P, De Vos M, Libert C
    J Biol Chem, 2014-12-23;290(7):4022-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Inhibition of activin A ameliorates skeletal muscle injury and rescues contractile properties by inducing efficient remodeling in female mice.
    Authors: Yaden B, Wang Y, Wilson J, Culver A, Milner A, Datta-Mannan A, Shetler P, Croy J, Dai G, Krishnan V
    Am J Pathol, 2014-04-01;184(4):1152-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Regulatory T-cells and cAMP suppress effector T-cells independently of PKA-CREM/ICER: a potential role for Epac.
    Authors: Vang, Amanda G, Housley, William, Dong, Hongli, Basole, Chaitali, Ben-Sasson, Shlomo Z, Kream, Barbara, Epstein, Paul M, Clark, Robert B, Brocke, Stefan
    Biochem J, 2013-12-15;456(3):463-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
    Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
    J. Exp. Med., 2006-11-20;203(12):2763-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human Lymphotoxin-alpha/TNF-beta Protein
By Anonymous on 11/06/2018
Application: Immunoassay Standard

The recombinant TNFb was used in an ELISA with a sensitivity of 6pg/ml.

Immunoassay Standard Recombinant Human Lymphotoxin-alpha/TNF-beta Protein 211-TBB