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Recombinant Human LTK Protein, CF

Catalog #: 9376-LK Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9376-LK-050
Recombinant Human LTK Protein Bioactivity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human LTK Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human LTK is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human FAM-150A that produces 50% of the optimal binding response is 40-240 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human LTK protein
Ser20-Pro424, with a C-terminal His-tag
Accession #
P29376-1
N-terminal Sequence
Analysis
Ser20
Predicted Molecular Mass
42 kDa
SDS-PAGE
56-84 kDa, reducing conditions

Product Datasheets

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9376-LK

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9376-LK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human LTK Protein Bioactivity View Larger

When Recombinant Human LTK (Catalog # 9376-LK) is immobilized at 2 μg/mL, 100 μL/well, Recombinant Human FAM-150A binds with an ED50 of 40-240 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LTK

LTK, also known as Leukocyte tyrosine kinase receptor and protein tyrosine kinase 1, is a type I transmembrane protein and shares a high degree of sequence similarity with kinases in the insulin receptor family (1). Alternative splicing generates several isoforms of 50-100 kDa (2). LTK contains a 408 aa extracellular domain, 25 aa transmembrane domain, and 415 aa cytoplasmic domain. The cytoplasmic region has several phosphotyrosines that interact with downstream signaling molecules (3). LTK is reported to undergo dimerand trimerization when under certain conditions. The extracellular domain of human LTK shares 75% homology with mouse LTK. It is expressed in preB lymphocytes, B lymphocytes, and other hematopoietic cells, as well as brain and placenta (48). Human LTK can bind to PLCgamma 1, PI3K, GAP and Raf1 in vivo (6). LTK promotes neurite outgrowth via PI3K/AKT and Ras/MAPK pathways (7). FAM150A and FAM150B can both bind to the extracellular domain of LTK, but only FAM150A is the specific ligand for LTK (9, 10).

References
  1. Honda, H. et al. (1999) Oncogene 18:3821.
  2. Yazaki, Y. et al. (1993) Proc Natl. Acad. Sci. U.S.A. 90:5404.
  3. Maru, Y., et al. (1990) Oncogene Res. 5:19.
  4. Ben-Neriah, Y. et al. (1988) Nature 333:672.
  5. Bernards, A. et al. (1990) EMBO J. 9:2279.
  6. Kozutsumi, H et al. (1994) Oncogene 9:2991.
  7. Yamada, S. et al. (2008) Neuroreport 19:1733.
  8. Roll, JD. et al. (2012) PLoS One 7:e31733.
  9. Reshetnyak, AV. et al. (2015) Proc Natl Acad Sci U S A. 112:15862.
  10. Guan, J, AV. et al. (2015) Elife. 4:e09811.
Long Name
Leukocyte Tyrosine Kinase Receptor
Entrez Gene IDs
4058 (Human); 17005 (Mouse); 311337 (Rat)
Alternate Names
EC 2.7.10.1; leukocyte receptor tyrosine kinase; leukocyte tyrosine kinase receptor; leukocyte tyrosine kinase; LTK; TYK1; TYK1Protein tyrosine kinase 1

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