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Recombinant Human LIMPII/SR-B2 Fc Chimera Protein, CF

Catalog #: 1966-LM
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1966-LM-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (4)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human LIMPII/SR-B2 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Glucosylceramidase/GBA (Catalog # 7410-GHB) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human LIMPII/SR-B2 Fc Chimera (Catalog # 1966-LM) binds with an ED50 of 100-800 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human LIMPII/SR-B2 protein
Human LIMPII
(Arg27-Thr432)
Accession # Q14108		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
Q14108
N-terminal Sequence
Analysis
Arg27
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
73 kDa (monomer)
SDS-PAGE
105-115 kDa, reducing conditions

Product Datasheets

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1966-LM

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1966-LM

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LIMPII/SR-B2

LIMPII (Lysosomal Integral Membrane Protein II), also known as LPG85 (85 kDa lysosomal membrane sialoglycoprotein) and as CD36 antigen-like 2 (CD36L2), is a major lysosomal membrane protein. It belongs to the scavenger receptor class B subfamily and is designated member 2 (SR-B2). Other mammalian members of this family include SR-B1 (alternatively known as Cla-1 and CD36L1), and SR-B3 (CD36) (1-3). SR-B/CD36 family members are type III integral membrane proteins with an N- as well as a C-terminal cytoplasmic tail, and a large extracellular (or lumenal in the case of LIMPII) loop containing similarly spaced cysteine residues and multiple glycosylation sites. The C-terminal cytoplasmic tail has a di-leucine-based motif that mediates effective lysosomal targeting. LIMPII is widely expressed on all tissues and cell types so far examined. It is also expressed on the surface of activated platelets. LIMPII binds thrombospondin-1, but the biological significance of this interaction is not known. LIMPII-thrombospondin interaction may contribute to the pro-adhesive changes of activated platelets during coagulation, and inflammation (1). Overexpression of LIMPII causes an enlargement of early endosomes and late endosomes, suggesting that LIMPII may play a role in lysosome/endosome biogenesis (4). Mice deficient in LIMPII are impaired in membrane transport processes, resulting in ureteric pelvic junction obstruction, deafness and peripheral neuropathy (5).

References
  1. Crombie, R. and R. Silverstein (1998) J. Biol. Chem. 273:4855. 
  2. Febbraio, M. et al. (2001) J. Clin. Invest. 108:785.
  3. Eskelinen, E-L. et al. (2003) Trends in Cell Biology 13:137.
  4. Kuronita, T. et al. (2002) J. Cell Sci. 115:4117.
  5. Gamp, A-C. et al. (2003) Human Molecular Genetics 12:631.
Long Name
Lysosomal Integral Membrane Protein II
Entrez Gene IDs
950 (Human); 12492 (Mouse)
Alternate Names
AMRF; CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2(lysosomal integral membrane protein II); CD36 antigen; CD36L2; CD36L2LIMP II; HLGP85; LGP85; LIMP-2,85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2; LIMPII; LIMPIICD36 antigen-like 2; LPG85; lysosome membrane protein 2; Lysosome membrane protein II; SCARB2; Scavenger receptor class B member 2,85 kDa lysosomal membrane sialoglycoprotein; scavenger receptor class B, member 2; SRB2; SR-B2; SR-BII

Citations for Recombinant Human LIMPII/SR-B2 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Single Neutralizing Monoclonal Antibodies Targeting the VP1 GH Loop of Enterovirus 71 Inhibit both Virus Attachment and Internalization during Viral Entry.
    Authors: Ku Z, Ye X, Shi J, Wang X, Liu Q, Huang Z
    J Virol, 2015-09-23;89(23):12084-95.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: Bioassay
  2. Human SCARB2-dependent infection by coxsackievirus A7, A14, and A16 and enterovirus 71.
    Authors: Yamayoshi S, Iizuka S, Yamashita T
    J. Virol., 2012-03-21;86(10):5686-96.
    Species: Virus
    Sample Types: Virus
    Applications: Immunoprecipitation
  3. Identification of a Human SCARB2 Region That Is Important for Enterovirus 71 Binding and Infection.
    Authors: Yamayoshi S, Koike S
    J. Virol., 2011-03-09;85(10):4937-46.
    Species: Human
    Sample Types: Protein
    Applications: Immunoprecipitation
  4. Scavenger receptor B2 is a cellular receptor for enterovirus 71.
    Authors: Yamayoshi S, Yamashita Y, Li J, Hanagata N, Minowa T, Takemura T, Koike S
    Nat. Med., 2009-06-21;15(7):798-801.
    Species: Human
    Sample Types: Peptide
    Applications: Bioassay

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