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Recombinant Human LILRB5/CD85c/LIR-8 Fc Chimera Protein, CF

Catalog #: 10410-T4 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
10410-T4-050
Recombinant Human LILRB5/CD85c/LIR-8 Fc Chimera Protein Binding Activity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human LILRB5/CD85c/LIR-8 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human LILRB5/CD85c/LIR-8 Fc Chimera (Catalog # 10410-T4) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with an ED50 of 0.03-0.24 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human LILRB5/CD85c/LIR-8 protein
Human LILRB5/CD85c/LIR-8
(Gly24-Gly458)
Accession # O75023.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
O75023.1
N-terminal Sequence
Analysis
Gly24
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
74 kDa
SDS-PAGE
85-105 kDa, under reducing conditions

Product Datasheets

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10410-T4

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10410-T4

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human LILRB5/CD85c/LIR-8 Fc Chimera Protein Binding Activity View Larger

When Recombinant Human LILRB5/CD85c/LIR-8 Fc Chimera (Catalog # 10410-T4) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin-like 7 (Catalog # 914-AN) binds with an ED50 of 0.03-0.24 µg/mL.

SDS-PAGE Recombinant Human LILRB5/CD85c/LIR-8 Fc Chimera Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Human LILRB5/CD85c/LIR-8 Fc Chimera (Catalog # 10410-T4) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 85-105 kDa and 170-210 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LILRB5/CD85c/LIR-8

LIR8, also known as CD85c and LILRB5, belongs to the Leukocyte immunoglobulin-like receptors (LILR) family of transmembrane glycoproteins involved in regulating immune responses (1,2). There are at least thirteen LILR family members and are divided into activating (LILRA) or inhibiting (LILRB) molecules (1,2). Mature human LIR8 consists of an extracellular domain (ECD) with four Ig-like domains, a transmembrane segment, and a cytoplasmic domain with two immunoreceptor tyrosine-based inhibitory motifs (ITIM). Alternative splicing of human LIR8 generates at least 2 isoforms, one lacking the second Ig-like domain. The LILR family appears to be primate-specific receptors in terms of sequence homology. LIR8 is expressed on NK cells and in the tryptic granules of mast cells and negatively regulates immune cell activation (3, 4). It is present on the mast cell surface following cell activation and degranulation (4). Activated mast cells may also release soluble forms of LIR8 (3). LIR8 has also been shown to be expressed on T cells and induce CD8+ T cell proliferation (5).  Consistent with the demonstrated binding of LILRB2 to Angiopoietin-like 2 and 5 (6), R&D Systems in-house testing indicates that LIR8 binds to Angiopoietin-like 7. Recently, a common missense variant of LIR8 was found to be associated with statin intolerance and myalgia (7).

References
  1. Brown, D. et al. (2004) Tissue antigens. 64:215.
  2. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  3. Borges, L. et al. (1997) J. Immunol. 159:5192.
  4. Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
  5. Hogan, H. E. et al. (2016) Sci Rep. 6:21780.
  6. Zheng, J. et al. (2012) Nature 485:656.
  7. Siddigui, M. et al. (2017) Eur. Heart J. 38:3569.
Long Name
Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM And ITIM Domains), Member 5
Entrez Gene IDs
10990 (Human); 102129427 (Cynomolgus Monkey)
Alternate Names
CD85 antigen-like family member C; CD85c antigen; CD85c; Leukocyte immunoglobulin-like receptor 8; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB5; LIR8; LIR-8; LIR8CD85C; LIR-8leukocyte immunoglobulin-like receptor subfamily B member 5; member 5

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