Recombinant Human Layilin Protein Summary
Product Specifications
Ala22-Glu220, with a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2536-LA
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
2536-LA/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Layilin
Layilin (named after the L-A-Y-I-L-I six amino acid motif in the molecule's transmembrane segment) is a 55 kDa member of the animal C-type lectin family. The human Layilin cDNA encodes a 374 amino acid (aa) type I transmembrane protein that, based on the orthologous molecule in hamster, contains a 19 aa signal sequence, a 201 aa extracellular region, a 31 aa transmembrane segment, and a 123 aa cytoplasmic tail (1, 2). The extracellular domain has a 130 aa carbohydrate recognition domain that contains only one potential Ca++-binding site. The intracellular region contains three types of LH (layilin homology) repeats. There are three 16‑18 aa LH1 repeats, 3 five aa (E-G-S-F/W-V) LH2 repeats, and 2 four aa (N-D/E-I-Y) LH3 repeats. Adjacent LH2-LH3 tandem arrays provide docking sites for talin, a cytoskeleton-membrane adaptor protein (3). The human layilin extracellular region is 85% aa identical to the hamster layilin extracellular region, and 82% aa identical to the extracellular region of a similar molecule identified in mouse. Layilin is known to be a receptor for hyaluronan (HA), a negatively charged linear high molecular weight polysaccharide composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine (1, 4, 5). HA is found in the extracellular matrix of most animal tissues and in body fluids. Thus, it may be that layilin modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease. Layilin binds specifically to HA but not to other glycosaminoglycans (4).
- Strausberg, R. (2002) GenBank Accession # AAH25407.
- Borowsky, M.L. and R.O. Hynes (1998) J. Cell Biol. 143:429.
- Rees, D.J.G. et al. (1990) Nature 347:685.
- Bono, P. et al. (2001) Mol. Biol. Cell 12:891.
- Goa, K.L. and P. Benfield (1994) Drugs 47:536.
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human Layilin Protein
Average Rating: 4 (Based on 1 Review)
Have you used Recombinant Human Layilin Protein?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
