Recombinant Human Langerin/CD207 Protein, CF
Recombinant Human Langerin/CD207 Protein, CF Summary
Product Specifications
Tyr64-Pro328, with an N-terminal 9-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2088-LN
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Langerin/CD207
Langerin (also known as CD207) is a type II transmembrane glycoprotein which is member K of the C-type lectin domain family 4 (1). Langerin is used as a marker for Langerhans cells (LCs) which represent the immature dendritic cells in the epidermis (1, 2). LCs uniquely contain “tennis racket”-shaped endosomal recycling compartment subdomains with pentalamellar membranes termed Birbeck granules (1 - 3). Langerin is necessary and sufficient for Birbeck granule formation (1). The 328 amino acid (aa) human langerin sequence contains a 43 aa cytoplasmic domain, a 21 aa transmembrane domain and a 264 aa extracellular domain (ECD) that contains a coiled-coil domain and a single C-type lectin domain. Trimerization greatly increases the lectin binding affinity (4). Langerin internalizes endogenous proteins such as type I procollagen. Internalization by LC is thought to lead to suppression of self reactions (4 - 6). Langerin also mediates endocytosis of non-peptide antigens containing mannose, N-acetyl glucosamine and fucose that are expressed by mycobacteria and fungae (4, 7). Some antigens, such as the M. leprae glycolipid arabinomycolate, are ultimately presented by human LC CD1a in cutaneous-draining lymph nodes (8). Langerin performs a barrier-like function to HIV-1 transmission due to its internalization of virus particles for destruction (9). A rare human polymorphism within the lectin domain, W264R, abolishes both carbohydrate recognition and Birbeck granule formation (10, 11). Genetic deletion of mouse langerin was not shown to have functional consequence other than abolishing Birbeck granule formation (12). Human langerin shares 68%, 62%, 71% aa identity with mouse, rat and bovine langerin ECD, respectively.
- Valladeau, J. et al. (2000) Immunity 12:71.
- Valladeau, J. et al. (2003) Immunol. Res. 28:93.
- McDermott, R. et al. (2002) Mol. Biol. Cell 13:317.
- Stambach, N. S. and M. E. Taylor (2003) Glycobiology 13:401.
- Tada, Y. et al. (2006) J. Invest. Dermatol. 126:1549.
- Ritter, U. and A. Osterloh (2007) Med. Microbiol. Immunol. 196:51.
- Takahara, K. et al. (2003) Int. Immunol. 16:819.
- Hunger, R. E. et al. (2004) J. Clin. Invest. 113:701.
- De Witte, L. et al. (2007) Nat. Med. 13:367.
- Verdijk, P. et al. (2005) J. Invest. Dermatol. 124:714.
- Ward, E. M. et al. (2006) J. Biol. Chem. 281:15450.
- Kissenpfennig, A. et al. (2005) Mol. Cell. Biol. 25:88.
Citations for Recombinant Human Langerin/CD207 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Differential recognition of oligomannose isomers by glycan-binding proteins involved in innate and adaptive immunity
Authors: C Gao, K Stavenhage, B Eckmair, TR McKitrick, AY Mehta, Y Matsumoto, AM McQuillan, MS Hanes, D Eris, KJ Baker, N Jia, M Wei, J Heimburg-M, B Ernst, RD Cummings
Science Advances, 2021-06-09;7(24):.
Species: Human
Sample Types: Protein Extracts
Applications: Bioassay -
Langerhans Cell-Dendritic Cell Cross-Talk via Langerin and Hyaluronic Acid Mediates Antigen Transfer and Cross-Presentation of HIV-1.
Authors: van den Berg L, Cardinaud S, van der Aar A, Sprokholt J, de Jong M, Zijlstra-Willems E, Moris A, Geijtenbeek T
J Immunol, 2015-07-13;195(4):1763-73.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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