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Recombinant Human Langerin/CD207 Fc Chimera Protein, CF

Catalog #: 10401-LN Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
10401-LN-050
Recombinant Human Langerin/CD207 Fc Chimera Protein Binding Activity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Langerin/CD207 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Langerin/CD207 Fc Chimera (Catalog # 10401-LN) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Mannose-Polyacrylamide binds with an ED50 of 20-160 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Langerin/CD207 protein
MDHuman IgG1
(Pro100-Lys330)		IEGR Human Langerin/CD207
(Pro65-Pro328)
Accession # Q9UJ71.2
N-terminusC-terminus
Accession #
Q9UJ71.2
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
56 kDa
SDS-PAGE
58-71 kDa, under reducing conditions

Product Datasheets

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10401-LN

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10401-LN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human Langerin/CD207 Fc Chimera Protein Binding Activity View Larger

When Recombinant Human Langerin/CD207 Fc Chimera (10401-LN) is immobilized at 0.2 μg/mL (100 μL/well), Biotinylated Mannose-Polyacrylamide binds with an ED50 of 20-160 ng/mL.

SDS-PAGE Recombinant Human Langerin/CD207 Fc Chimera Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Human Langerin/CD207 Fc Chimera (Catalog # 10401-LN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 58-71 kDa and 116-142 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Langerin/CD207

Langerin (also known as CD207) is a transmembrane glycoprotein within the type II C-Type lectin receptor family and has been identified as a pathogen binding receptor for immune regulation (1). Human langerin consists of an extracellular domain (ECD) containing a coiled-coil domain and a single C-type lectin domain, a transmembrane domain and a short cytoplasmic domain with a proline-rich motif. The mature ECD of human langerin shares 68%, 62%, 71% amino acid identity with mouse, rat and bovine langerin ECD, respectively. Langerin is used as a marker for Langerhans cells (LCs) which represent the immature dendritic cells in the epidermis (1, 2). LCs uniquely contain "tennis racket"-shaped endosomal recycling compartment subdomains with pentalamellar membranes termed Birbeck granules (1-3). Langerin is necessary and sufficient for Birbeck granule formation (1). Trimerization greatly increases the lectin binding affinity (4). Langerin internalizes endogenous proteins such as type I procollagen. Internalization by LC is thought to lead to suppression of self-reactions (4-6). Langerin also mediates endocytosis of non-peptide antigens containing mannose, N-acetyl glucosamine and fucose that are expressed by mycobacteria and fungae (4, 7). Some antigens, such as the M. leprae glycolipid arabinomycolate, are ultimately presented by human LC CD1a in cutaneous-draining lymph nodes (8). Langerin performs a barrier-like function to HIV-1 transmission due to its internalization of virus particles for destruction (9). A rare human polymorphism within the lectin domain, W264R, abolishes both carbohydrate recognition and Birbeck granule formation (10, 11). Genetic deletion of mouse langerin was not shown to have functional consequence other than abolishing Birbeck granule formation (12).

References
  1. Valladeau, J. et al. (2000) Immunity 12:71.
  2. Valladeau, J. et al. (2003) Immunol. Res. 28:93.
  3. McDermott, R. et al. (2002) Mol. Biol. Cell 13:317.
  4. Stambach, N. S. and M. E. Taylor (2003) Glycobiology 13:401.
  5. Tada, Y. et al. (2006) J. Invest. Dermatol. 126:1549.
  6. Ritter, U. and A. Osterloh (2007) Med. Microbiol. Immunol. 196:51.
  7. Takahara, K. et al. (2003) Int. Immunol. 16:819.
  8. Hunger, R. E. et al. (2004) J. Clin. Invest. 113:701.
  9. De Witte, L. et al. (2007) Nat. Med. 13:367.
  10. Verdijk, P. et al. (2005) J. Invest. Dermatol. 124:714.
  11. Ward, E. M. et al. (2006) J. Biol. Chem. 281:15450.
  12. Kissenpfennig, A. et al. (2005) Mol. Cell. Biol. 25:88.
Entrez Gene IDs
50489 (Human); 246278 (Mouse)
Alternate Names
CD207 antigen; CD207 antigen, langerin; CD207 molecule, langerin; CD207; CLEC4KLangerhans cell specific c-type lectin; C-type lectin domain family 4 member K; C-type lectin domain family 4, member K; Langerin

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