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Recombinant Human L-Selectin/CD62L Fc Chimera Protein, CF

Catalog #: 728-LS
12 Citations Datasheet / COA / SDS
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Recombinant Human L-Selectin/CD62L Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of LS180 human colorectal adenocarcinoma cells. When 5 x 104 cells/well are added to Recombinant Human L‑Selectin/CD62L Fc Chimera coated plates, adhesion is induced in a dose dependent manner after 1 hour incubation at 37 °C. The ED50 for this effect is 0.4-2 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human L-Selectin/CD62L protein
Human L-Selectin
(Trp39 - Asn332)
Accession # P14151		 IEGRMD Human IgG1
(Pro100 - Lys330)		 6-His tag
N-terminus C-terminus
Accession #
P14151
N-terminal Sequence
Analysis
Trp39
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
60.4 kDa (monomer)
SDS-PAGE
90-100 kDa, reducing conditions

Product Datasheets

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Product Datasheet
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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

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Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 0.1 mg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: L-Selectin/CD62L

L-Selectin (also known as Leukocyte Selectin, LAM‑1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L), a member of the Selectin family, is a cell surface glycoprotein expressed constitutively on a wide variety of leukocytes. Two forms of L‑Selectin have been reported, apparently arising as a result of post-translational modifications. The lymphocyte form shows an apparent molecular weight of 74 kDa, while the neutrophil form is 90 - 100 kDa. Human and mouse L‑Selectin share 76% amino acid (aa) sequence homology.

L‑Selectin plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites. Acting in cooperation with P-Selectin and E-Selectin, L‑Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic "rolling" of the leukocytes on the endothelium. This initial interaction, also involving ICAM-1 and VCAM-1, leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble L‑Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of L‑Selectin may be elevated or lowered in subjects with a variety of pathological conditions.

Entrez Gene IDs
6402 (Human); 20343 (Mouse); 29259 (Rat); 102142895 (Cynomolgus Monkey)
Alternate Names
CD62L antigen; CD62L; gp90-MEL; hLHRc; LAM1; LAM-1; LAM1LECAM1; LECAM1; LEU8; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LNHRTQ1; LSEL; L-Selectin; LYAM1; Lyam-1; LYAM1CD62 antigen-like family member L; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; PLNHR; selectin L; SELL; TQ1

Citations for Recombinant Human L-Selectin/CD62L Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Lung-Derived Selectins Enhance Metastatic Behavior of Triple Negative Breast Cancer Cells
    Authors: SU Khan, Y Xia, D Goodale, G Schoettle, AL Allan
    Biomedicines, 2021-10-30;9(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Doxycycline-Dependent Self-Inactivation of CRISPR-Cas9 to Temporally Regulate On- and Off-Target Editing
    Authors: A Kelkar, Y Zhu, T Groth, G Stolfa, AB Stablewski, N Singhi, M Nemeth, S Neelamegha
    Mol. Ther., 2019-09-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues
    Authors: EW Martin, R Malgor, VA Resto, DJ Goetz, MM Burdick
    Sci Rep, 2019-06-11;9(1):8511.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  4. Dynamic biochemical tissue analysis detects functional L-selectin ligands on colon cancer tissues
    Authors: GE Carlson, EW Martin, VS Shirure, R Malgor, VA Resto, DJ Goetz, MM Burdick
    PLoS ONE, 2017-03-10;12(3):e0173747.
    Applications: Bioassay
  5. Development of Polymer Microcapsules Functionalized with Fucoidan to Target P-Selectin Overexpressed in Cardiovascular Diseases
    Authors: Bo Li
    Adv Healthc Mater, 2016-12-12;0(0):.
    Applications: Bioassay
  6. Non-fucosylated CB CD34(+) cells represent a good target for enforced fucosylation to improve engraftment following cord blood transplantation
    Authors: Simon N Robinson
    Cytotherapy, 2016-12-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity
    Sci Rep, 2016-11-28;6(0):37953.
    Applications: Bioassay
  8. Using CRISPR-Cas9 to quantify the contributions of O-glycans, N-glycans and Glycosphingolipids to human leukocyte-endothelium adhesion
    Sci Rep, 2016-07-26;6(0):30392.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Glycopeptide analogues of PSGL-1 inhibit P-selectin in vitro and in vivo.
    Authors: Krishnamurthy V, Sardar M, Ying Y, Song X, Haller C, Dai E, Wang X, Hanjaya-Putra D, Sun L, Morikis V, Simon S, Woods R, Cummings R, Chaikof E
    Nat Commun, 2015-03-31;6(0):6387.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Heterotropic modulation of selectin affinity by allosteric antibodies affects leukocyte rolling.
    Authors: Riese S, Kuehne C, Tedder T, Hallmann R, Hohenester E, Buscher K
    J Immunol, 2014-01-15;192(4):1862-9.
    Species: Human
    Sample Types: Protein, Whole Cells
    Applications: Bioassay, Flow Cytometry
  11. Circulating tumor cells from prostate cancer patients interact with E-selectin under physiologic blood flow.
    Authors: Gakhar, Gunjan, Navarro, Vicente, Jurish, Madelyn, Lee, Guang Yu, Tagawa, Scott T, Akhtar, Naveed H, Seandel, Marco, Geng, Yue, Liu, He, Bander, Neil H, Giannakakou, Paraskev, Christos, Paul J, King, Michael, Nanus, David M
    PLoS ONE, 2013-12-27;8(12):e85143.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. A role for the L-selectin adhesion system in mediating cytotrophoblast emigration from the placenta.
    Authors: Prakobphol A, Genbacev O, Gormley M, Kapidzic M, Fisher SJ
    Dev. Biol., 2006-06-15;298(1):107-17.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot (Detection)

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