Recombinant Human Klotho beta Protein, CF Summary
Product Specifications
Phe53-Leu997, with a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5889-KB
| Formulation | Supplied as a 0.2 μm filtered solution in PBS, Glycerol and EDTA. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Human FGF-21 (2539-FG) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Klotho beta His-tag (Catalog # 5889-KB) binds with an ED50 of 2.5-20 ng/mL.
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1 μg/lane of Recombinant Human Klotho beta was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a single band at 113-125 kDa.
Reconstitution Calculator
Background: Klotho beta
Klotho beta, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue (1, 2). Like Klotho, Klotho beta facilitates binding between FGF19 subfamily members and their receptors via formation of a ternary complex (3). The Klotho beta mediated interaction of human FGF19 (mouse FGF15) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1) (2-5). Klotho beta is also a cofactor for the interaction of FGF21 with FGF Receptor 1c in adipocytes, which allows FGF21 to stimulate GLUT1 expression, upregulating adipocyte insulin-dependent glucose uptake (2-4, 6). The 1043 amino acid (aa) type I transmembrane protein is composed of a 51 aa signal sequence, a 943 aa extracellular domain (ECD) containing two glycosidase-like regions, a 21 aa transmembrane domain, and 28 aa intracellular tail. Since Klotho-related proteins lack critical active site Glu residues present in beta -glycosidases, it was initially unclear whether they were functional enzymes (1, 7). However, glucuronidase activity has since been demonstrated for Klotho, indicating that physiologically relevant enzymatic activity for Klotho beta is also possible (8). The extracellular domain shares 79%, 87%, 87% and 67% identity with mouse, equine, canine and rat Klotho beta, respectively. The low identity with rat reflects aa discordance within rodent ECD.
- Mian, I. S. (1998) Blood Cells Mol. Dis. 24:83.
- Kurosu, H. and M. Kuro-o (2009) Mol. Cell. Endocrinol. 299:72.
- Ito, S. et al. (2005) J. Clin. Invest. 115:2202.
- Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687.
- Lin, B. C. et al. (2007) J. Biol. Chem. 282:27277.
- Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci USA 104:7432.
- Chang, Q. et al. (2005) Science 310:490.
- Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.
Citations for Recombinant Human Klotho beta Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Heating-mediated purification of active FGF21 and structure-based design of its variant with enhanced potency
Authors: YE Jung, KW Lee, JH Cho, DW Bae, BG Jeong, YJ Jung, SB Park, YJ An, K Kim, GS Lee, LW Kang, JH Moon, JH Lee, EK Kim, HS Yim, SS Cha
Scientific Reports, 2023-01-18;13(1):1005.
Species: Human
Sample Types: Protein
Applications: SPR -
FGF21-FGFR4 signaling in cardiac myocytes promotes concentric cardiac hypertrophy in mouse models of diabetes
Authors: C Yanucil, D Kentrup, X Li, A Grabner, K Schramm, EC Martinez, J Li, I Campos, B Czaya, K Heitman, D Westbrook, AR Wende, A Sloan, JM Roche, A Fornoni, MS Kapiloff, C Faul
Scientific Reports, 2022-05-05;12(1):7326.
Species: Human
Sample Types: Recombinant Proteins
Applications: Bioassay -
Curtailing FGF19's mitogenicity by suppressing its receptor dimerization ability
Authors: J Niu, J Zhao, J Wu, G Qiao, J Gu, C Zhou, Q Li, L Ying, D Wang, H Lin, X Li, M Mohammadi, Z Huang
Proc Natl Acad Sci U S A, 2020-11-03;0(0):.
Species: Human
Sample Types: Protein
Applications: SPR -
betaKlotho is identified as a target for theranostics in non-small cell lung cancer
Authors: F Li, X Li, Z Li, W Ji, S Lu, W Xia
Theranostics, 2019-10-12;9(25):7474-7489.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Molecular elements in FGF19 and FGF21 defining KLB/FGFR activity and specificity
Authors: A Agrawal, S Parlee, D Perez-Tilv, P Li, J Pan, PA Mroz, AM Kruse Hans, B Andersen, B Finan, A Kharitonen, RD DiMarchi
Mol Metab, 2018-05-11;13(0):45-55.
Species: Human
Sample Types: Protein
Applications: Bioassay -
The transcription factor MITF is a critical regulator of GPNMB expression in dendritic cells.
Authors: Gutknecht M, Geiger J, Joas S, Dorfel D, Salih H, Muller M, Grunebach F, Rittig S
Cell Commun Signal, 2015-03-24;13(0):19.
Species: Human
Sample Types: Whole Cells
Applications: Control -
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
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Why is an antibody used to immobilize Recombinant Human Klotho-beta protein, Catalog # 5889-KB, in the binding assay as described in the insert?
The Recombinant Human Klotho-beta protein is formulated in PBS, 50%(v/v) Glycerol and 0.1 mM EDTA, pH 6.8. This formulation results in lower efficiency of binding of the protein by itself and therefore an antibdy is used to immobilize the protein. Additionally, the protocol as described is more sensitive for detection of FGF-21 binding.
Reviews for Recombinant Human Klotho beta Protein, CF
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