Home / KIR2DS4/CD158i / Recombinant Human KIR2DS4/CD158i Fc Chimera Protein, CF

Recombinant Human KIR2DS4/CD158i Fc Chimera Protein, CF

Catalog #: 1847-KR
2 Citations Datasheet / COA / SDS
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1847-KR-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (2)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human KIR2DS4/CD158i Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
Mouse myeloma cell line, NS0-derived human KIR2DS4/CD158i protein
Human KIR2DS4
(Gln22-His245)
Accession # P43632		IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
P43632
N-terminal Sequence
Analysis
No results obtained: Gln22 predicted
Predicted Molecular Mass
51.2 kDa (monomer)
SDS-PAGE
70-85 kDa, reducing conditions

Product Datasheets

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1847-KR

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1847-KR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KIR2DS4/CD158i

KIR2DS4 (2DS4), previously called NKAT-8 and designated CD158i, is a type I transmembrane glycoprotein that belongs to the killer cell Ig-like receptor (KIR) family (1). KIRs are expressed on CD56dim NK cells and T cell subsets where they differentiate normal from abnormal cells and regulate effector functions in the innate immune system (1-3). KIRs are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails. KIR2DS4 cDNA encodes 304 amino acids (aa) including a 21 aa signal sequence, a 224 aa ECD, a 20 aa transmembrane sequence and a 39 aa cytoplasmic domain. KIR receptors have no structural orthologs in nonprimates, although mouse Ly-49 proteins perform similar functions (2). Like other activating KIRs, KIR2DS4 has a short tail and a positively charged amino acid (aa) within the transmembrane domain that interacts with the ITAM-bearing signaling adaptor, DAP12 (2, 3). Expression of activating KIR is balanced with inhibitory KIR to impart specificity to the immune response. KIR2DS4 recognizes six HLA-C allotypes and two HLA-A allotypes (A*1101 and A*1102) but no HLA-B allotypes (4, 5). It has also been suggested that KIR2DS4 recognizes a non-MHC ligand, based on specific binding to melanoma cells that lack MHC Class I (6). A common allele, KIR2DS4*003, is present in up to 89% of Caucasians and ~30% of Asians; it creates a prematurely terminated, soluble, inactive form (7, 8). Lack of functional KIR2DS4 can potentially create unbalanced inhibition of killer cell function (4, 8).

References
  1. Bottino, C. et al. (1996) Eur. J. Immunol. 26:1816.
  2. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
  3. Purdy, A.K. and K.S. Campbell (2009) Cancer Biol. Ther. 8:13.
  4. Graef, T. et al. (2009) J. Exp. Med. 206:2557.
  5. Katz, G. et al. (2001) J. Immunol. 166:7260.
  6. Katz, G. et al. (2004) J. Immunol. 173:1819.
  7. Maxwell, L.D. et al. (2004) Hum. Immunol. 65:613.
  8. Middleton, D. et al. (2007) Hum. Immunol. 68:128.
Long Name
Killer Cell Immunoglobulin-like Receptor, Two Domains, Short Cytoplasmic Tail, 4
Entrez Gene IDs
3809 (Human)
Alternate Names
CD158 antigen-like family member I; CD158i antigen; CD158i; cl-39; killer cell immunoglobulin-like receptor 2DS4; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail4,nkat8; killer inhibitory receptor 4-1-2; KIR antigen 2DS4; KIR2DS4; KKA3KIR1D; MGC120019; MGC125315; MGC125317; MHC class I NK cell receptor; natural killer cell inhibitory receptor; Natural killer-associated transcript 8; NKAT-8; NKAT8KIR412; P58 natural killer cell receptor clones CL-39/CL-17; p58 NK receptor CL-39/CL-17

Citations for Recombinant Human KIR2DS4/CD158i Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Human NK cell receptor KIR2DS4 detects a conserved bacterial epitope presented by HLA-C
    Authors: MJW Sim, S Rajagopala, DM Altmann, RJ Boyton, PD Sun, EO Long
    Proc. Natl. Acad. Sci. U.S.A., 2019-05-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. High affinity anti-TIM-3 and anti-KIR monoclonal antibodies cloned from healthy human individuals
    Authors: S Ryser, A Estellés, E Tenorio, LM Kauvar, ML Gishizky
    PLoS ONE, 2017-07-19;12(7):e0181464.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay

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