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Recombinant Human Integrin alpha 11 beta 1 Protein, CF

Catalog #: 6357-AB
1 Citation Datasheet / COA / SDS
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6357-AB-050
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Integrin alpha 11 beta 1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Collagen I at 10 µg/mL (100 µL/well) can bind Recombinant Human Integrin alpha 11 beta 1 with an apparent Kd <2.0 nM.
Optimal dilutions should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 11 beta 1 protein
Human Integrin alpha 11
(Phe23-Pro1141)
(Leu524Arg)
Accession # Q9UKX5

GGGSGGGSGGGS

 Acidic Tail HHHHHH
Human Integrin beta 1
(Gln21-Asp728)
Accession # P05556

GGGSGGGSGGGS

 Basic Tail
N-terminus C-terminus


N-terminal Sequence
Analysis
Phe23 ( alpha 11) & Gln21 (predicted, not observed) ( beta 1)
Structure / Form
Noncovalently-linked heterodimer
Predicted Molecular Mass
134.6 kDa ( alpha 11) & 88.4 ( beta 1)
SDS-PAGE
160-180 kDa & 120-140 kDa, reducing conditions

Product Datasheets

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6357-AB

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6357-AB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 11 beta 1

Integrin alpha 11 beta 1 is a noncovalent, heterodimeric collagen receptor that contains a 150 kDa alpha 11 subunit complexed to a 130 kDa beta 1/CD29 subunit.  alpha 11 beta 1 is the only known alpha 11-containing integrin. By contrast, at least twelve alpha -integrins share the beta 1 subunit (1-5). alpha 11 beta 1 is expressed on fibroblasts from cornea, skin and periodontal ligament (3‑9). It is also expressed on mesenchymal stem cells and is considered a marker for non‑muscle mesenchymal tissue (3, 8‑10). Expression of alpha 11 beta 1 is stimulated by TGF‑ beta 1, activin A, and type I interferons, and down‑regulated by FGF-2 (6, 9‑11). The alpha 11 extracellular domain (ECD) contains a vWFA domain which includes the ligand binding site (2‑5). The beta 1 ECD also contains a vWFA domain, which participates in binding. Each subunit has a transmembrane sequence and a short cytoplasmic tail. The dimer assumes a folded configuration at "rest". Divalent cations and intracellular (inside‑out) signaling convert it to an active, extended and open conformation (1, 2). The 1120 amino acid (aa) human alpha 11 ECD shares 90‑93% aa sequence identity with mouse, rat, equine, canine and bovine alpha 11, while the 708 aa human beta 1 ECD shares 92‑96% aa sequence identity with rat, bovine, mouse, and feline beta 1. I domain-containing beta 1 integrins alpha 1 beta 1, alpha 2 beta 1, alpha 10 beta 1 and alpha 11 beta 1 all bind collagens (3‑5). alpha 11 beta 1 preferentially binds interstitial collagens such as type I (5, 8, 12). It can contract collagen matrices and influence inflammatory edema (3, 13). Depending on context, alpha 11 beta 1 can support or inhibit cell migration on collagen (7, 8). It is frequently up‑regulated in the stroma of lung adenocarcinomas and enhances tumorigenicity (14, 15).

References
  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  3. Popova, S.N. et al. (2007) Acta Physiol. 190:179.
  4. Lehnert, K. et al. (1999) Genomics 60:179.
  5. Velling, T. et al. (1999) J. Biol. Chem. 274:25735.
  6. Gouttenoire, J. et al. (2010) Eur. J. Cell Biol. 89:307.
  7. Popova, S.N. et al. (2004) Dev. Biol. 270:427.
  8. Tiger, C-F. et al. (2001) Dev. Biol. 237:116.
  9. Carrecedo, S. et al. (2010) J. Biol. Chem. 285:10434.
  10. Varas, L. et al. (2007) Stem Cells Dev. 16:965.
  11. Coelho, L.F.L. et al. (2006) Cytokine 33:352.
  12. Zhang, W-M. et al. (2003) J. Biol. Chem. 278:7270.
  13. Svendsen, O.S. et al. (2009) Arterioscler. Thromb. Vasc. Biol. 29:1864.
  14. Wang, K-K. et al. (2002) Oncogene 21:7598.
  15. Zhu, C-Q. et al. (2007) Proc. Natl. Acad. Sci. USA 104:11754.
Alternate Names
Integrin alpha 11 beta 1

Citation for Recombinant Human Integrin alpha 11 beta 1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. α11?1 Integrin is Induced in a Subset of Cancer-Associated Fibroblasts in Desmoplastic Tumor Stroma and Mediates In Vitro Cell Migration
    Authors: C Zeltz, J Alam, H Liu, PM Erusappan, H Hoschuetzk, A Molven, H Parajuli, E Cukierman, DE Costea, N Lu, D Gullberg
    Cancers (Basel), 2019-06-01;11(6):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

FAQs

  1. What is the amino acid sequence of the acidic and basic tails?

    • Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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