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Recombinant Human IL-5 Protein

Catalog #: 205-IL
37 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
205-IL-005/CF
205-IL-025/CF

With Carrier

Catalog # Availability Size / Price Qty
205-IL-005
205-IL-025
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Recombinant Human IL-5 Protein Bioactivity
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Citations (37)
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Recombinant Human IL-5 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.04-0.2 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (stably transfected)-derived human IL-5 protein
Ile20-Ser134
Accession #
P05113
N-terminal Sequence
Analysis
Ile20
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
13 kDa (monomer)
SDS-PAGE
13-15 kDa, reducing conditions

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205-IL (with carrier)

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205-IL/CF (carrier free)

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COA
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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

205-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

205-IL/CF

Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Bioactivity Recombinant Human IL-5 Protein Bioactivity View Larger

Recombinant Human IL-5 (Catalog # 205-IL) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.04-0.2 ng/mL.

SDS-PAGE Recombinant Human IL-5 Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human IL-5 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 13-15 kDa and 28-32 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-5

Interleukin-5 (IL-5) is a secreted glycoprotein that belongs to the alpha -helical group of cytokines (1 ‑ 3). Unlike other family members, it is present as a covalently linked antiparallel dimer (4, 5). The cDNA for human IL-5 encodes a signal peptide and a 115 amino acid (aa) mature protein. Mature human IL-5 shares 70%, 70%, 62%, 71%, 70% and 66%, aa sequence identity with mouse, rat, canine, equine, feline and porcine IL-5, respectively and shows cross‑reactivity with mouse IL-5. IL-5 is primarily produced by CD4+ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, Reed-Sternberg cells in Hodgkin’s disease, and IL‑2‑stimulated invariant natural killer T cells (iNKT) (1 ‑ 3, 6 ‑ 8). IL-5 increases production and mobilization of eosinophils and CD34+ progenitors from the bone marrow and causes maturation of eosinophil precursors outside the bone marrow (1, 6, 9, 10). The receptor for human IL-5, mainly expressed by eosinophils, but also found on basophils and mast cells, consists of a unique ligand-binding subunit (IL-5 R alpha ) and a shared signal‑transducing subunit, beta c (3, 6, 11). IL-5 R alpha first binds IL-5 at low affinity, then associates with preformed beta c dimers, forming a high-affinity receptor (12). IL-5 also binds proteoglycans, potentially enhancing its activity (13). Soluble forms of IL-5 R alpha   antagonize IL-5 and can be found in vivo (10, 14). In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells (1 ‑ 3, 9, 10, 15, 16). IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release (17).

References
  1. Rosenberg, H. F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
  2. Elsas, P.X. and M. I. G. Elsas (2007) Curr. Med. Chem. 14:1925.
  3. Martinez-Moczygemba, M. and D. P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  4. Minamitake, Y. et al. (1990) J. Biochem. 107:292.
  5. McKenzie, A. N. et al. (1991) Mol. Immunol. 28:155.
  6. Shakoory, B. et al. (2004) J. Interferon Cytokine Res. 24:271.
  7. Lalani, T. et al. (1999) Ann. Allergy Asthma Immunol. 82:317.
  8. Sakuishi, K. et al. (2007) J. Immunol. 179:3452.
  9. Clutterbuck, E. J. et al. (1989) Blood 73:1504.
  10. Cameron, L. et al. (2000) J. Immunol. 164:1538.
  11. Tavernier, J. et al. (1991) Cell 66:1175.
  12. Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.
  13. Lipscombe, R. et al. (1998) J. Leukocyte Biol. 63:342.
  14. Tavernier, J. et al. (2000) Blood 95:1600.
  15. Kopf, M. et al. (1996) Immunity 4:15.
  16. Horikawa, K. and K. Takatsu (2006) Immunology 118:497.
  17. Denburg, J. A. et al. (1991) Blood 77:1462.
Long Name
Interleukin 5
Entrez Gene IDs
3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
Alternate Names
BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I

Citations for Recombinant Human IL-5 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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  1. Localization and RNA Interference-Driven Inhibition of a Brugia malayi-Encoded Interleukin-5 Receptor Binding Protein
    Authors: R Mejia, S Bennuru, Y Oksov, S Lustigman, G Munirathin, R Kalyanasun, TB Nutman
    Infection and Immunity, 2022-04-25;0(0):e0031721.
    Species: Nematode - Brugia malayi
    Sample Types: Complex Sample Type
    Applications: ELISA Detection
  2. Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis
    Authors: Y Imoto, T Takabayash, M Sakashita, Y Kato, K Yoshida, M Kidoguchi, K Koyama, N Adachi, Y Kimura, K Ogi, Y Ito, M Kanno, M Okamoto, N Narita, S Fujieda
    Biomolecules, 2020-11-18;10(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation
    Authors: J Yan, SP Pandey, BJ Barnes, JR Turner, C Abraham
    Cell Rep, 2020-06-30;31(13):107820.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Single-site, five-year experience with human eosinophil isolation by density gradient centrifugation and CD16 immunomagnetic negative separation
    Authors: Y Cao, S Shin, DJ Carroll, JA O'Sullivan, BS Bochner
    BMC Res Notes, 2020-04-10;13(1):211.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  5. Fibrinogen Is a Specific Trigger for Cytolytic Eosinophil Degranulation
    Authors: ME Coden, LF Loffredo, MT Walker, BM Jeong, K Nam, BS Bochner, H Abdala-Val, S Berdnikovs
    J. Immunol., 2019-12-09;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. The role of IL-36? and its regulation in eosinophilic inflammation in allergic rhinitis
    Authors: X Qin, M Liu, S Zhang, C Wang, T Zhang
    Cytokine, 2019-03-01;117(0):84-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Control of cytokine-driven eosinophil migratory behavior by TGF-beta-induced protein (TGFBI) and periostin.
    Authors: Karina T Barretto, Calvin M Swanson, Christopher L Nguyen, Douglas S Annis, Stephane J Esnault, Deane F Mosher, Mats W Johansson
    PLoS ONE, 2018-07-26;0(0):1932-6203.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Myeloid-derived growth factor is a resident endoplasmic reticulum protein
    Authors: V Bortnov, DS Annis, FJ Fogerty, KT Barretto, KB Turton, DF Mosher
    J. Biol. Chem., 2018-06-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Interleukin-5 suppresses Vascular Endothelial Growth Factor-induced angiogenesis through STAT5 signaling
    Authors: F Bucher, J Lee, S Shin, MM Kim, YS Oh, S Ha, H Zhang, K Yea
    Cytokine, 2018-06-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. IL-3 but not monomeric IgE regulates Fc?RI levels and cell survival in primary human basophils
    Authors: F Zellweger, P Buschor, G Hobi, D Brigger, CA Dahinden, PM Villiger, A Eggel
    Cell Death Dis, 2018-05-01;9(5):510.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Activation of Human Basophils by A549 Lung Epithelial Cells Reveals a Novel IgE-Dependent Response Independent of Allergen
    Authors: JT Schroeder, AP Bieneman
    J. Immunol., 2017-06-26;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. IL-5-stimulated eosinophils adherent to periostin undergo stereotypic morphological changes and ADAM8-dependent migration
    Authors: MW Johansson, M Khanna, V Bortnov, DS Annis, CL Nguyen, DF Mosher
    Clin. Exp. Allergy, 2017-05-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Human eosinophils constitutively express a unique serine protease, PRSS33
    Authors: S Toyama, N Okada, A Matsuda, H Morita, H Saito, T Fujisawa, S Nakae, H Karasuyama, K Matsumoto
    Allergol Int, 2017-02-16;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Functional IL-33 receptors are expressed in early progenitor stages of allergy-related granulocytes
    Immunology, 2016-09-30;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. The effect of hepatocyte growth factor on secretory functions in human eosinophils
    Cytokine, 2016-08-20;88(0):45-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Impaired P2X1 Receptor-Mediated Adhesion in Eosinophils from Asthmatic Patients
    J Immunol, 2016-05-09;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Eosinophil-specific deletion of IkappaBalpha in mice reveals a critical role of NF-kappaB-induced Bcl-xL for inhibition of apoptosis.
    Authors: Schwartz C, Willebrand R, Huber S, Rupec R, Wu D, Locksley R, Voehringer D
    Blood, 2015-04-10;125(25):3896-904.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Functional analysis of free fatty acid receptor GPR120 in human eosinophils: implications in metabolic homeostasis.
    Authors: Konno, Yasunori, Ueki, Shigehar, Takeda, Masahide, Kobayashi, Yoshiki, Tamaki, Mami, Moritoki, Yuki, Oyamada, Hajime, Itoga, Masamich, Kayaba, Hiroyuki, Omokawa, Ayumi, Hirokawa, Makoto
    PLoS ONE, 2015-03-19;10(3):e0120386.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model.
    Authors: Yao, Xiujuan, Wang, Wei, Li, Yan, Huang, Ping, Zhang, Qian, Wang, Jingjing, Wang, Wen, Lv, Zhe, An, Yunqing, Qin, Jianguo, Corrigan, Chris J, Huang, Kewu, Sun, Yongchan, Ying, Sun
    Respir Res, 2015-03-18;16(0):39.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Physiologic concentrations of HMGB1 have no impact on cytokine-mediated eosinophil survival or chemotaxis in response to Eotaxin-2 (CCL24).
    Authors: Dyer, Kimberly, Rosenberg, Helene F
    PLoS ONE, 2015-03-16;10(3):e0118887.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. SOCS3 silencing attenuates eosinophil functions in asthma patients.
    Authors: Zafra M, Canas J, Mazzeo C, Gamez C, Sanz V, Fernandez-Nieto M, Quirce S, Barranco P, Ruiz-Hornillos J, Sastre J, del Pozo V
    Int J Mol Sci, 2015-03-10;16(3):5434-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Cholesterol selectively regulates IL-5 induced mitogen activated protein kinase signaling in human eosinophils.
    Authors: Burnham M, Esnault S, Roti Roti E, Bates M, Bertics P, Denlinger L
    PLoS ONE, 2014-08-14;9(8):e103122.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Natural killer cells induce eosinophil activation and apoptosis.
    Authors: Awad A, Yassine H, Barrier M, Vorng H, Marquillies P, Tsicopoulos A, Duez C
    PLoS ONE, 2014-04-11;9(4):e94492.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Tumour necrosis factor-alpha regulates human eosinophil apoptosis via ligation of TNF-receptor 1 and balance between NF-kappaB and AP-1.
    Authors: Kankaanranta H, Ilmarinen P, Zhang X, Adcock I, Lahti A, Barnes P, Giembycz M, Lindsay M, Moilanen E
    PLoS ONE, 2014-02-28;9(2):e90298.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Cytokines alter IgA1 O-glycosylation by dysregulating C1GalT1 and ST6GalNAc-II enzymes.
    Authors: Suzuki, Hitoshi, Raska, Milan, Yamada, Koshi, Moldoveanu, Zina, Julian, Bruce A, Wyatt, Robert J, Tomino, Yasuhiko, Gharavi, Ali G, Novak, Jan
    J Biol Chem, 2014-01-07;289(8):5330-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Pin1-FADD interactions regulate Fas-mediated apoptosis in activated eosinophils.
    Authors: Oh, Jiyoung, Malter, James S
    J Immunol, 2013-04-19;190(10):4937-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Osteopontin is expressed and functional in human eosinophils.
    Authors: Puxeddu I, Berkman N, Ribatti D
    Allergy, 2009-10-05;65(2):168-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
    Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
    Clin. Chem., 2007-11-01;53(11):2010-2.
    Applications: ELISA (Standard)
  29. cIAP-2 and survivin contribute to cytokine-mediated delayed eosinophil apoptosis.
    Authors: Vassina EM, Yousefi S, Simon D, Zwicky C, Conus S, Simon HU
    Eur. J. Immunol., 2006-07-01;36(7):1975-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006-06-16;120(2):220-31.
    Applications: ELISA (Standard)
  31. Urokinase-type plasminogen activator modulates airway eosinophil adhesion in asthma.
    Authors: Brooks AM, Bates ME, Vrtis RF, Jarjour NN, Bertics PJ, Sedgwick JB
    Am. J. Respir. Cell Mol. Biol., 2006-05-25;35(4):503-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Pharmacological characterization of guinea pig chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2).
    Authors: Liu F, Gonzalo JA, Manning S, O&amp;apos;Connell LE, Fedyk ER, Burke KE, Elder AM, Pulido JC, Cao W, Tayber O, Qiu Y, Ghosh S, Ocain TD, Hodge MR, Suzuki-Yagawa Y
    Prostaglandins Other Lipid Mediat., 2005-05-01;76(1):133-47.
    Species: Guinea Pig
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Protection of human conjunctival fibroblasts from NO-induced apoptosis by interleukin-4 or interleukin-13.
    Authors: Fujitsu Y, Fukuda K, Kimura K, Seki K, Kumagai N, Nishida T
    Invest. Ophthalmol. Vis. Sci., 2005-03-01;46(3):797-802.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways.
    Authors: Kao CY, Chen Y, Thai P, Wachi S, Huang F, Kim C, Harper RW, Wu R
    J. Immunol., 2004-09-01;173(5):3482-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Eosinophils adhere to vascular cell adhesion molecule-1 via podosomes.
    Authors: Johansson MW, Lye MH, Barthel SR, Duffy AK, Annis DS, Mosher DF
    Am. J. Respir. Cell Mol. Biol., 2004-06-25;31(4):413-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Regulation of the interleukin-5 receptor alpha-subunit on peripheral blood eosinophils from healthy subjects.
    Authors: Hellman C, 107334, Hallden G, Hylander B, Lundahl J
    Lagging strand gap suppression connects BRCA-mediated fork protection to nucleosome assembly through PCNA-dependent CAF-1 recycling, 2003-01-01;131(1):75-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells.
    Authors: Liu X, Kohyama T, Wang H, Zhu YK, Wen FQ, Kim HJ, Romberger DJ, Rennard SI
    Am. J. Physiol. Lung Cell Mol. Physiol., 2002-05-01;282(5):L1049-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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