Recombinant Human IL-3R alpha Fc Chimera Avi-tag Protein, CF

Catalog #: AVI11140 Datasheet / COA / SDS

Biotinylated

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AVI11140-025

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-3R alpha Fc Chimera Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.50 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Human IL-3 (Catalog # 203-IL) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human IL-3R alpha/CD123 Fc Chimera Avi-tag (Catalog # AVI11140) binds with an ED₅₀ of 3.00-45.0 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-3R alpha protein

Human IL-3RA (Thr19-Arg305) Accession # P26951.1	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

P26951.1

N-terminal Sequence
Analysis

Thr19 and Lys20

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

61 kDa

SDS-PAGE

83-93 kDa, under reducing conditions.

Product Datasheets

AVI11140

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11140

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human IL-3 (203-IL) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human IL-3R alpha/CD123 Fc Chimera Avi-tag Protein (Catalog # AVI11140) binds with an ED₅₀ of 3.00-45.0 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human IL-3R alpha/CD123 Fc Chimera Avi-tag Protein (Catalog # AVI11140) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 83-93 kDa and 170-190 kDa, respectively.

Reconstitution Calculator

Background: IL-3R alpha

IL-3 receptor chain alpha (IL‑3R alpha) also known as CD123, is a member of the type 1 cytokine receptor family of proteins expressed mainly by activated T cells or mast cells (1-3). IL-3R alpha is a 60-70 kDa receptor component that binds IL-3 with low affinity and forms one-half of the IL-3 receptor complex. IL-3R alpha associates with the 130-140 kDa non-ligand binding IL-3R beta for high-affinity binding to IL-3 to form the heterodimeric receptor complex (4). IL-3R alpha consists of an extracellular domain (ECD) with an N-terminal Ig-like C2-type domain and a cytokine-binding domain containing two fibronectin type-III domains with a WSXWS motif, a helical transmembrane segment and a cytoplasmic domain (3). Within the mature ECD, human IL-3R alpha shares 30% and 31% amino acid sequence identity with mouse and rat IL-3R alpha, respectively. An isoform lacking the N-terminal domain of ECD has been identified in mouse and human (5, 6). IL‑3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage‑committed cells (7). IL-3R alpha is expressed on multiple cell types, including endothelial cells, monocytes, eosinophils, basophils plus mast cells, and plasmacytoid CD4+ T cells (8). IL-3R alpha is over-expressed in acute myeloid leukemia and this is associated with a poor prognosis in AML (9). Our Avi-tag Biotinylated human IL-3R alpha Fc chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Broughton, S.E. et al. (2012) Immunol. Rev. 250:277.
Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
Broughton, S.E. et al. (2014) Acta Crystallogr F Struc Biol Commun. 70:358.
Broughton, S.E. et al. (2014) Cell Reports. 8:410.
Chen, J. et al. (2009) J. Biol. Chem. 284:5763.
Hamming, O.J. et al. (2012) J Biol Chem. 287:9454.
Ogorochi, T. and A. Miyajima (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, ed. Oxford University, New York p. 40.
Michalska, A. et al. (2020) Postepy Dermatol Alergol. 37:299.
Testa, U. et al. (2002) Blood 100:2980.

Long Name

Interleukin 3 Receptor alpha

Entrez Gene IDs

3563 (Human); 16188 (Mouse); 102138639 (Cynomolgus Monkey)

Alternate Names

CD123 antigen; CD123; hIL3Ra; hIL-3Ra; IL-3 R alpha; IL-3 receptor subunit alpha; IL3R alpha; IL-3R alpha; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor, alpha (low affinity); interleukin-3 receptor subunit alpha; MGC34174