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Recombinant Human IL-36 gamma/IL-1F9 (aa 18-169) Protein

Catalog #: 6835-IL
10 Citations 2 Reviews Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
6835-IL-010/CF

With Carrier

Catalog # Availability Size / Price Qty
6835-IL-010
Recombinant Human IL-36 gamma/IL-1F9 (aa 18-169) Protein Bioactivity
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Product Details
Citations (10)
FAQs
Reviews (2)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-36 gamma/IL-1F9 (aa 18-169) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for for this effect is 1.5-9 ng/mL
Source
E. coli-derived human IL-36 gamma/IL-1F9 protein
Ser18-Asp169
Accession #
Q9NZH8
N-terminal Sequence
Analysis
Ser18
Predicted Molecular Mass
17 kDa
SDS-PAGE
17 kDa, reducing conditions

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6835-IL (with carrier)

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6835-IL/CF (carrier free)

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COA
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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6835-IL

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, TCEP and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in Water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

6835-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, TCEP and EDTA.
Reconstitution Reconstitute at 200 μg/mL in Water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human IL-36 gamma/IL-1F9 (aa 18-169) Protein Bioactivity View Larger

Recombinant Human IL-36 gamma/IL-1F9 (Catalog # 6835-IL) induces IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 1.5-9 ng/mL.

SDS-PAGE Recombinant Human IL-36 gamma/IL-1F9 (aa 18-169) Protein SDS-PAGE View Larger

1 µg/lane of Recombinant Human IL-36 gamma /IL-1F9 (aa 18-169) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-36 gamma/IL-1F9

IL-36 gamma [previously called IL-1F9, IL-1 epsilon (epsilon), and IL-1H1] is a member of the IL-1 family which includes IL‑1 beta, IL‑1 alpha, IL‑1ra, IL‑18, IL‑36 Ra (IL‑1F5), IL-36 alpha (IL‑1F6), IL‑36 beta (IL‑1F8), IL‑37 (IL‑1F7) and IL-1F10 (1‑5). All family members show a 12 beta ‑strand, beta ‑trefoil configuration, and are believed to have arisen from a common ancestral gene (2, 3). IL‑36 gamma is an 18‑22 kDa, 169 amino acid (aa) intracellular and secreted protein that contains no signal sequence, no prosegment and no potential N‑linked glycosylation sites (1, 2, 4, 6, 7). Human IL‑36 gamma (aa 18‑169) shares 58%, 59%, 68% and 69% aa sequence identity with mouse, rat, bovine and equine IL‑36 gamma, respectively, and 23‑57% aa sequence identity with other family members. A 134 aa isoform missing aa 19‑53 has been reported (8). Highest levels of IL‑36 gamma are produced by Langerhans cells, keratinocytes, and stomach Chief cells and parietal cells; these cells contribute to first‑line defense against pathogens in the skin, lungs and digestive tract (2, 3, 6, 9). Its expression is induced by LPS treatment of monocytes, and by IL‑ alpha / beta, IL‑17 or TNF‑ alpha treatment of keratinocytes and bronchial epithelia (1, 6, 7, 9‑11). Skin IL-36 gamma expression is increased in contact hypersensitivity and psoriasis (1, 6, 11). It is elevated in inflammatory disorders of the lung (such as asthma) and viral infections. Lung IL‑36 gamma and other IL‑36 proteins contribute to neutrophil influx (4, 7, 10). The receptor for IL-36 gamma is a combination of IL‑1 Rrp2, mainly found in epithelia and keratinocytes, and the widely expressed IL‑1 RAcP (4, 7, 9). IL‑36 alpha,  beta  and  gamma all activate NF‑ kappa B and MAPK pathways in an IL‑1 Rrp2 dependent manner, and IL‑36 gamma induces production of inflammatory cytokines and chemokines such as CXCL8/IL‑8 (7, 9, 10).

References
  1. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  2. Busfield, S.J. et al. (2000) Genomics 66:213.
  3. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  4. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
  5. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
  6. Debets, R. et al. (2001) J. Immunol. 167:1440.
  7. Chustz, R.T. et al. (2010) Am. J. Respir. Cell Mol. Biol. Sept. 24 [Epub ahead of print]
  8. SwissProt Accession # Q9NZH8.
  9. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
  10. Ramadas, R.A. et al. (2011) Am. J. Respir. Cell Mol. Biol. 44:134.
  11. Johnston, A. et al. (2011) J. Immunol. 186:2613.
Long Name
Interleukin 36 gamma/Interleukin 1 Family 9
Entrez Gene IDs
56300 (Human); 215257 (Mouse); 499744 (Rat)
Alternate Names
IL-1 epsilon; IL-1 H1; IL-1 Related Protein 2; IL-1(EPSILON); IL1E; IL-1-epsilon; IL1F9; IL-1F9; IL1H1; IL-1H1; IL-1-Related Protein 2; IL1RP2; IL-1rp2; IL36 gamma; IL-36 gamma; IL36G; interleukin 1 family, member 9; interleukin 1-related protein 2; Interleukin 36, Gamma; Interleukin-1 epsilon; interleukin-1 family member 9; Interleukin-1 homolog 1; Interleukin-36 Gamma

Citations for Recombinant Human IL-36 gamma/IL-1F9 (aa 18-169) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. The CDK4/6-EZH2 pathway is a potential therapeutic target for psoriasis
    Authors: A Müller, A Dickmanns, C Resch, K Schäkel, S Hailfinger, M Dobbelstei, K Schulze-Os, D Kramer
    J. Clin. Invest., 2020-11-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. IL-36? regulates mediators of tissue homeostasis in epithelial cells
    Authors: JE Heath, GM Scholz, PD Veith, EC Reynolds
    Cytokine, 2019-03-08;119(0):24-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Cathepsin G cleaves and activates IL-36? and promotes the inflammation of psoriasis
    Authors: J Guo, J Tu, Y Hu, G Song, Z Yin
    Drug Des Devel Ther, 2019-02-08;13(0):581-588.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. IL-38 has an anti-inflammatory action in psoriasis and its expression correlates with disease severity and therapeutic response to anti-IL-17A treatment
    Authors: L Mercurio, M Morelli, C Scarponi, EZ Eisenmesse, N Doti, G Pagnanelli, E Gubinelli, C Mazzanti, A Cavani, M Ruvo, CA Dinarello, C Albanesi, S Madonna
    Cell Death Dis, 2018-10-30;9(11):1104.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Intramuscular vaccination of guinea pigs with the live-attenuated human herpes simplex vaccine VC2 stimulates a transcriptional profile of vaginal Th17 and regulatory Tr1 responses
    Authors: BA Stanfield, PJF Rider, J Caskey, F Del Piero, KG Kousoulas
    Vaccine, 2018-04-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. RNA-Seq Analysis of IL-1B and IL-36 Responses in Epidermal Keratinocytes Identifies a Shared MyD88-Dependent Gene Signature
    Authors: WR Swindell, MA Beamer, MK Sarkar, S Loftus, J Fullmer, X Xing, NL Ward, LC Tsoi, MJ Kahlenberg, Y Liang, JE Gudjonsson
    Front Immunol, 2018-01-29;9(0):80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Whole Cells
  7. IL-36? inhibits differentiation and induces inflammation of keratinocyte via Wnt signaling pathway in psoriasis
    Authors: W Wang, X Yu, C Wu, H Jin
    Int J Med Sci, 2017-08-18;14(10):1002-1007.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Structural and functional attributes of the Interleukin-36 receptor
    Authors: Guanghui Yi
    J Biol Chem, 2016-06-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Signal Transduction and Intracellular Trafficking by the Interleukin 36 Receptor.
    Authors: Saha S, Singh D, Raymond E, Ganesan R, Caviness G, Grimaldi C, Woska J, Mennerich D, Brown S, Mbow M, Kao C
    J Biol Chem, 2015-08-12;290(39):23997-4006.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Application of IL-36 receptor antagonist weakens CCL20 expression and impairs recovery in the late phase of murine acetaminophen-induced liver injury.
    Authors: Scheiermann P, Bachmann M, Hardle L, Pleli T, Piiper A, Zwissler B, Pfeilschifter J, Muhl H
    Sci Rep, 2015-02-17;5(0):8521.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human IL-36 gamma/IL-1F9 (aa 18-169) Protein

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Recombinant Human IL-36 gamma/IL-1F9 (aa 18-169) Protein
By Anonymous on 12/06/2021
Application: In vitro bioactivity in cell culture
In vitro bioactivity in cell culture Recombinant Human IL-36 gamma/IL-1F9 (aa 18-169) Protein 6835-IL
Recombinant Human IL-36 gamma/IL-1F9 (aa 18-169) Protein
By Anonymous on 12/24/2017
Application: Characterization prior to use.