Recombinant Human IL-24 Protein Summary
Product Specifications
Met28-Leu206, with a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1965-IL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1965-IL/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: IL-24
Interleukin 24 (IL-24), also known as mda-7 (melanoma differentiation associated gene-7), is a member of the IL-10 family of helical cytokines. The IL-24 gene encodes a precursor protein of 207 amino acids (aa) that contains a 48 aa signal sequence and an 18 kDa, 158 aa mature segment. There are three potential N-linked glycosylation sites, at least one of which is used. When secreted, IL-24 is a 35 - 40 kDa phosphorylated glycoprotein that apparently can exist as either a monomer or dimer. It is suggested that glycosylation is essential for activity. Mature human IL-24 shares 69% aa sequence identity with mouse and rat IL-24. Human IL-24 is also active in rodent systems. Cells known to express IL-24 include B cells, CD4+ T cells, NK cells, lymph node dendritic cells, monocytes, melanocytes, and melanoma cells. Functionally, IL‑24 has diverse activities. At low concentrations on monocytes, it induces type I proinflammatory cytokines such as IFN‑ gamma, IL‑1 beta, IL‑12 and TNF-alpha. At high concentrations, it is a strong inducer of apoptosis in tumor cells, but not normal cells. IL‑24 also has anti-angiogenic properties. It directly binds IL-24 receptors on endothelial cells, activating STAT3 and blocking their differentiation. IL-24 binds and signals through two heterodimeric receptor complexes. One complex is the combination of IL‑20 R alpha and IL-20 R beta, which is shared with IL-19 and IL‑20. The second complex is a combination of IL‑22 R and IL-20 R beta, which is shared with IL-20.
- Jiang, H. et al. (1995) Oncogene 11:2477.
- Jiang, H. et al. (1996) Proc. Natl. Acad. Sci. USA 93:9160.
- Wang, M. et al. (2002) J. Biol Chem. 227:7341.
- Chada, S. et al. (2004) Int. Immunopharmacol. 4:649.
- Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
- Chen, J. et al. (2003) Molec. Ther. 8:220.
Citations for Recombinant Human IL-24 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Single-cell analysis of psoriasis resolution demonstrates an inflammatory fibroblast state targeted by IL-23 blockade
Authors: Francis, L;McCluskey, D;Ganier, C;Jiang, T;Du-Harpur, X;Gabriel, J;Dhami, P;Kamra, Y;Visvanathan, S;Barker, JN;Smith, CH;Capon, F;Mahil, SK;
Nature communications
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A new insight into the role of aryl hydrocarbon receptor (AhR) in the migration of glioblastoma by AhR-IL24 axis regulation
Authors: Y Liu, Y Chen, R Sha, Y Li, T Xu, X Hu, L Xu, Q Xie, B Zhao
Environment international, 2021-05-31;154(0):106658.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Characterization of IL-19, -20, and -24 in acute and chronic kidney diseases reveals a pro-fibrotic role of IL-24
Authors: D Pap, A Veres-Szék, B Szebeni, R Rokonay, A Ónody, R Lippai, IM Takács, A Tislér, M Kardos, F Oswald, A Fekete, AJ Szabó, Á Vannay
J Transl Med, 2020-04-19;18(1):172.
Species: Human, Rat
Sample Types: Whole Cells
Applications: Bioassay -
Integrated microRNA/mRNA expression profiling of the skin of psoriasis patients
Authors: D Deli?, K Wolk, R Schmid, O Gabrielyan, D Christou, K Rieber, M Rolser, I Jakob, F Wiech, M Griesser, C Wohnhaas, G Kokolakis, E Witte-Händ, P Baum, R Sabat
J. Dermatol. Sci., 2019-11-30;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Modulation of Th1/Tc1 and Th17/Tc17 responses in pulmonary tuberculosis by IL-20 subfamily of cytokines
Authors: NP Kumar, K Moideen, VV Banurekha, D Nair, S Babu
Cytokine, 2018-04-21;108(0):190-196.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Iron-induced calcification in human aortic vascular smooth muscle cells through interleukin-24 (IL-24), with/without TNF-alpha
Authors: S Kawada, Y Nagasawa, M Kawabe, H Ohyama, A Kida, N Kato-Kogoe, M Nanami, Y Hasuike, T Kuragano, H Kishimoto, K Nakasho, T Nakanishi
Sci Rep, 2018-01-12;8(1):658.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IL-24 Promotes Pseudomonas aeruginosa Keratitis in C57BL/6 Mouse Corneas
Authors: BX Ross, N Gao, X Cui, TJ Standiford, J Xu, FX Yu
J. Immunol, 2017-03-22;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Microarray Analysis Reveals Increased Expression of Matrix Metalloproteases and Cytokines of Interleukin-20 Subfamily in the Kidneys of Neonate Rats Underwent Unilateral Ureteral Obstruction: A Potential Role of IL-24 in the Regulation of Inflammation and Tissue Remodeling
Authors: D Pap, E Sziksz, Z Kiss, R Rokonay, A Veres-Szék, R Lippai, IM Takács, É Kis, A Fekete, G Reusz, A Vannay, AJ Szabó
Kidney Blood Press. Res, 2017-02-28;42(1):16-32.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
MMP8 Is Increased in Lesions and Blood of Acne Inversa Patients: A Potential Link to Skin Destruction and Metabolic Alterations
Authors: Robert Sabat
Mediators Inflamm., 2016-10-23;2016(0):4097574.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Tumor necrosis factor receptor signaling in keratinocytes triggers interleukin-24-dependent psoriasis-like skin inflammation in mice.
Authors: Kumari S, Bonnet M, Ulvmar M, Wolk K, Karagianni N, Witte E, Uthoff-Hachenberg C, Renauld J, Kollias G, Toftgard R, Sabat R, Pasparakis M, Haase I
Immunity, 2013-11-07;39(5):899-911.
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay -
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Induction of phenotype modifying cytokines by FERMT1 mutations.
Authors: Heinemann A, He Y, Zimina E, Boerries M, Busch H, Chmel N, Kurz T, Bruckner-Tuderman L, Has C
Hum. Mutat., 2011-03-01;32(4):397-406.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Interleukin-24 inhibits the plasma cell differentiation program in human germinal center B cells.
Authors: Maarof G, Bouchet-Delbos L, Gary-Gouy H, Durand-Gasselin I, Krzysiek R, Dalloul A
Blood, 2009-11-24;115(9):1718-26.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression.
Authors: Tohyama M, Hanakawa Y, Shirakata Y, Dai X, Yang L, Hirakawa S, Tokumaru S, Okazaki H, Sayama K, Hashimoto K
Eur. J. Immunol., 2009-10-01;39(10):2779-88.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages.
Authors: Duluc D, Corvaisier M, Blanchard S, Catala L, Descamps P, Gamelin E, Ponsoda S, Delneste Y, Hebbar M, Jeannin P
Int. J. Cancer, 2009-07-15;125(2):367-73.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IL-24 modulates IFN-gamma expression in patients with tuberculosis.
Authors: Wu B, Huang C, Kato-Maeda M, Hopewell PC, Daley CL, Krensky AM, Clayberger C
Immunol. Lett., 2007-12-26;117(1):57-62.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Systemic cancer gene therapy using adeno-associated virus type 1 vector expressing MDA-7/IL24.
Authors: Tahara I, Miyake K, Hanawa H, Kurai T, Hirai Y, Ishizaki M, Uchida E, Tajiri T, Shimada T
Mol. Ther., 2007-06-05;15(10):1805-11.
Applications: ELISA (Standard) -
The effects of IL-20 subfamily cytokines on reconstituted human epidermis suggest potential roles in cutaneous innate defense and pathogenic adaptive immunity in psoriasis.
Authors: Sa SM, Valdez PA, Wu J
J. Immunol., 2007-02-15;178(4):2229-40.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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