Recombinant Human IL-17E Protein
Recombinant Human IL-17E Protein Summary
Product Specifications
Tyr33-Gly177, with an N-terminal Met
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1258-IL
Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
Reconstitution | Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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1258-IL/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
Reconstitution | Reconstitute at 100 μg/mL in sterile 4 mM HCl. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
![Recombinant Human IL-17E Protein Bioactivity Recombinant Human IL-17E Protein Bioactivity](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/protein/1258-il_recombinant-human-il-17e-protein-bioactivity-272020132842.jpg)
Measured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED50 for this effect is 0.25-1.5 ng/mL.
Reconstitution Calculator
Background: IL-17E/IL-25
The Interleukin‑17 (IL‑17) family of proteins are immunoregulatory cytokines that share a conserved cysteine‑rich region. IL‑17E, which is also known as IL‑25, promotes Th2‑biased immune responses. This is in contrast to other IL‑17 family members which promote Th1- and Th17‑biased inflammation. IL‑25 is an important mediator of allergic reactions and protection against intestinal parasites (1, 2). Mature human IL‑25 shares 80% amino acid sequence identity with mouse and rat IL‑25 (3, 4). During helminth infections and allergic reactions, IL‑25 is locally up‑regulated in intestinal and airway epithelial cells, atopic dermatitis skin lesions, and local Th2 cells, eosinophils, and basophils (4‑9). It binds to IL‑17 RB but also requires IL‑17 RA to exert its activity (3, 8, 10). IL‑25 acts on a variety of cell types which respond with increased production of Th2 cytokines (e.g. IL‑4, IL‑5, IL‑13) and reduced production of Th1 and Th17 cytokines (e.g. IFN‑ gamma, IL‑12, IL‑23, IL‑17A, IL‑17F) (4‑6, 8, 9, 11‑15). Airway IL‑25 can be activated by MMP‑7, a protease that is up‑regulated in airway epithelium in response to allergen exposure (16). Cleaved IL‑25 shows enhanced binding to IL‑17 RB and stronger induction of Th2 cytokines (16). The Th2 cytokines, in turn, trigger expansion of Th2 memory cells and anti‑inflammatory M2 macrophages, increased eosinophil mobilization and activation, and dendritic cell migration (4, 6, 9, 13). These actions promote protective anti‑helminth immune responses (4, 5) as well as allergic inflammation and airway hyperreactivity (11). The IL‑25 induced suppression of Th1 and Th17 cytokines limits Th17 cell expansion and disease pathology in autoimmunity and colitis (12, 15). IL‑25 also promotes vascular endothelial cell proliferation and assembly into tubular structures (7). It supports the integrity of the blood‑brain barrier and limits CD4+ T cell infiltration into the brain (17).
- Saadoun, D. et al. (2011) Curr. Pharm. Des. 17:3781.
- Iwakura, Y. et al. (2011) Immunity 34:149.
- Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
- Fort, M.M. et al. (2001) Immunity 15:985.
- Zhao, A. et al. (2010) J. Immunol. 185:6921.
- Suzukawa, M. et al. (2012) J. Immunol. 189:3641.
- Corrigan, C.J. et al. (2011) Proc. Natl. Acad. Sci. USA 108:1579.
- Petersen, B.C. et al. (2012) Nat. Med. 18:751.
- Wang, Y.-H. et al. (2007) J. Exp. Med. 204:1837.
- Rickel, E.A. et al. (2008) J. Immunol. 181:4299.
- Hurst, S.D. et al. (2002) J. Immunol. 169:443.
- Kleinschek, M.A. et al. (2007) J. Exp. Med. 204:161.
- Cao, Q. et al. (2011) J. Am. Soc. Nephrol. 22:1229.
- Stock, P. et al. (2009) J. Immunol. 182:5116.
- Caruso, R. et al. (2009) Gastroenterology 136:2270.
- Goswami, S. et al. (2009) Nat. Immunol. 10:496.
- Sonobe, Y. et al. (2009) J. Biol. Chem. 284:31834.
Citations for Recombinant Human IL-17E Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury
Authors: G Xiao, R Kumar, Y Komuro, J Burguet, V Kakarla, I Azizkhania, SA Sheth, CK Williams, XR Zhang, M Macknicki, A Brumm, R Kawaguchi, P Mai, N Kaneko, HV Vinters, ST Carmichael, LA Havton, C DeCarli, JD Hinman
Cell Reports, 2022-12-20;41(12):111848.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
p38 MAPK signalling regulates cytokine production in IL-33 stimulated Type 2 Innate Lymphoid cells
Authors: T Petrova, J Pesic, K Pardali, M Gaestel, JSC Arthur
Sci Rep, 2020-02-26;10(1):3479.
Species: Human
Sample Types: Cells
Applications: Cell Culture -
Expression of c-Kit discriminates between two functionally distinct subsets of human type 2 innate lymphoid cells
Authors: T Hochdörfer, C Winkler, K Pardali, J Mjösberg
Eur. J. Immunol., 2019-03-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The role of IL-36? and its regulation in eosinophilic inflammation in allergic rhinitis
Authors: X Qin, M Liu, S Zhang, C Wang, T Zhang
Cytokine, 2019-03-01;117(0):84-90.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Interleukin-25 and eosinophils progenitor cell mobilization in allergic asthma
Authors: W Tang, SG Smith, W Du, A Gugilla, J Du, JP Oliveria, K Howie, BM Salter, GM Gauvreau, PM O'Byrne, R Sehmi
Clin Transl Allergy, 2018-02-13;8(0):5.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Effector and regulatory dendritic cells display distinct patterns of miRNA expression
Authors: V Lombardi, S Luce, H Moussu, L Morizur, C Gueguen, C Neukirch, S Chollet-Ma, L Mascarell, M Aubier, V Baron-Bodo, P Moingeon
Immun Inflamm Dis, 2017-05-12;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis.
Authors: Ling Y, Cypowyj S, Aytekin C, Galicchio M, Camcioglu Y, Nepesov S, Ikinciogullari A, Dogu F, Belkadi A, Levy R, Migaud M, Boisson B, Bolze A, Itan Y, Goudin N, Cottineau J, Picard C, Abel L, Bustamante J, Casanova J, Puel A
J Exp Med, 2015-04-27;212(5):619-31.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model.
Authors: Yao, Xiujuan, Wang, Wei, Li, Yan, Huang, Ping, Zhang, Qian, Wang, Jingjing, Wang, Wen, Lv, Zhe, An, Yunqing, Qin, Jianguo, Corrigan, Chris J, Huang, Kewu, Sun, Yongchan, Ying, Sun
Respir Res, 2015-03-18;16(0):39.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The differential regulation of human ACT1 isoforms by Hsp90 in IL-17 signaling.
Authors: Wu L, Wang C, Boisson B, Misra S, Rayman P, Finke J, Puel A, Casanova J, Li X
J Immunol, 2014-07-14;193(4):1590-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Composition of innate lymphoid cell subsets in the human skin: enrichment of NCR(+) ILC3 in lesional skin and blood of psoriasis patients.
Authors: Teunissen M, Munneke J, Bernink J, Spuls P, Res P, Te Velde A, Cheuk S, Brouwer M, Menting S, Eidsmo L, Spits H, Hazenberg M, Mjosberg J
J Invest Dermatol, 2014-03-21;134(9):2351-60.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Absence of a role for interleukin-13 in inflammatory bowel disease.
Authors: Biancheri P, Di Sabatino A, Ammoscato F, Facciotti F, Caprioli F, Curciarello R, Hoque S, Ghanbari A, Joe-Njoku I, Giuffrida P, Rovedatti L, Geginat J, Corazza G, MacDonald T
Eur J Immunol, 2014-02-01;44(2):370-85.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
An ACT1 mutation selectively abolishes interleukin-17 responses in humans with chronic mucocutaneous candidiasis.
Authors: Boisson B, Wang C, Pedergnana V, Wu L, Cypowyj S, Rybojad M, Belkadi A, Picard C, Abel L, Fieschi C, Puel A, Li X, Casanova J
Immunity, 2013-10-10;39(4):676-86.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IL-25 in atopic dermatitis: a possible link between inflammation and skin barrier dysfunction?
Authors: Hvid M, Vestergaard C, Kemp K
J. Invest. Dermatol., 2010-09-23;131(1):150-7.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Interleukin-25: a cytokine linking eosinophils and adaptive immunity in Churg-Strauss syndrome.
Authors: Terrier B, Bieche I, Maisonobe T, Laurendeau I, Rosenzwajg M, Kahn JE, Diemert MC, Musset L, Vidaud M, Sene D, Costedoat-Chalumeau N, Le Thi-Huong D, Amoura Z, Klatzmann D, Cacoub P, Saadoun D
Blood, 2010-08-20;116(22):4523-31.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Opposing roles of IL-17A and IL-25 in the regulation of TSLP production in human nasal epithelial cells.
Authors: Xu G, Zhang L, Wang DY, Xu R, Liu Z, Han DM, Wang XD, Zuo KJ, Li HB
Allergy, 2009-11-25;65(5):581-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages.
Authors: Duluc D, Corvaisier M, Blanchard S, Catala L, Descamps P, Gamelin E, Ponsoda S, Delneste Y, Hebbar M, Jeannin P
Int. J. Cancer, 2009-07-15;125(2):367-73.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities.
Authors: Rickel EA, Siegel LA, Yoon BR, Rottman JB, Kugler DG, Swart DA, Anders PM, Tocker JE, Comeau MR, Budelsky AL
J. Immunol., 2008-09-15;181(6):4299-310.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A novel IL-1 family cytokine, IL-33, potently activates human eosinophils.
Authors: Cherry WB, Yoon J, Bartemes KR, Iijima K, Kita H
J. Allergy Clin. Immunol., 2008-06-01;121(6):1484-90.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IL-17E upregulates the expression of proinflammatory cytokines in lung fibroblasts.
Authors: Létuvé S, Lajoie-Kadoch S, Audusseau S, Rothenberg ME, Fiset PO, Ludwig MS, Hamid Q
J. Allergy Clin. Immunol., 2006-02-08;117(3):590-6.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Interleukin-25-induced chemokines and interleukin-6 release from eosinophils is mediated by p38 mitogen-activated protein kinase, c-Jun N-terminal kinase, and nuclear factor-kappaB.
Authors: Wong CK, Cheung PF, Ip WK, Lam CW
Am. J. Respir. Cell Mol. Biol., 2005-04-28;33(2):186-94.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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We used this protein in an in-house ELISA along with mAb (1258) and pAb (AF1258) to quantify IL-17E in human serum and plasma. This combination detected IL-17E in our samples efficiently.
Reason for Rating: This recombinant protein works very well in vitro assay.