Recombinant Human IFN-alpha 6/IFNA6 Protein, CF Summary
Product Specifications
Ser21-Glu189
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
11168-IF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Recombinant Human IFN-alpha 6/IFNA6 Protein (Catalog # 11168-IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 1.00-30.0 pg/mL.
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2 μg/lane of Recombinant Human IFN-alpha 6/IFNA6 Protein (Catalog # 11168-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-22 kDa.
Reconstitution Calculator
Background: IFN-alpha 6/IFNA6
Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). Human IFNA2 was originally cloned in the early ‘80s and now more than a dozen closely related IFN alpha subtypes have been identified in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology (2-4). Structurally, type I IFNs belong to the class of five helical‑bundle cytokines, with the IFNA subtypes containing 2 conserved disulfide bonds (5). Mature human IFNA6, also known as IFN-alpha K, shares 56% aa sequence identity with mouse IFNA6. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit) (6, 7). Individual IFNA subtypes are known to display unique efficacies to viral protection, and IFNA6 has been shown to be a strong inducer of IFN-stimulated genes and anti-viral protection (8). IFNA6 displays high potency against metapneumovirus and inhibition of HIV infection (9).
- Pestka, S. et al. (1987) Annu Rev Biochem. 56:727
- Goeddel, D.V. et al. (1980) Nature 287:411.
- Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
- Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
- Wittling, M.C. et al. (2021) Front Immunol. 11:605673.
- van Pesch, V. et al. (2004) J. Virol. 78:8219.
- James, C.M. et al. (2007) Vaccine. 25(10):1856.
- Moll, H.P. et al. (2011) Cytokine. 53:52.
- George, J. and Mattapallil, J.J. (2018). Frontiers in immunology 9:299.
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