Recombinant Human Histamine N-Methyltransferase/HNMT, CF

Recombinant Human Histamine N-Methyltransferase/HNMT, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Histamine N-Methyltransferase/HNMT

Histamine N-Methyltransferase (HNMT) catalyzes the transfer of the methyl group of S-adenosyl-L-methionine to the N_e2 atom of the imidazole ring in histamine producing methylhistamine (1). Because histamine is a potent mediator of numerous biological events, including the development of allergic inflammation, termination of its action by N-methylation is important event. Two major pathways exist for the inactivation of histamine in mammals (2). The pathway of histamine inactivation through HNMT plays a major role in bronchial epithelial and endothelial cells of the human airways and gut and HNMT is the only enzyme responsible for termination of the neurotransmitter action of histamine in the brain. The human HNMT gene contains a common single nucleotide polymorphism (SNP) that codes for either threonine or isoleucine at residue 105 (3). The 105I variant has decreased activity and lower protein levels relative to 105T. This SNP does not pose a serious risk for histamine accumulation (4).

Related Research Areas

• Airway Inflammation and Remodeling
• Inflammatory Mediators
• Methyltransferases and Demethylases
• Neurotransmitters, Neuroactive Molecules and Associated Enzymes