Home / USP15 / Recombinant Human His6-USP15 Protein, CF

Recombinant Human His6-USP15 Protein, CF

Catalog #: E-594
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
E-594-050
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (3)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human His6-USP15 Protein, CF Summary

Product Specifications

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity

Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human USP15 concentration of 5-20 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555).

Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP15 protein
Met1 - Asn981 with a C-terminal 6-His tag
Accession #
Q9Y4E8.3
Predicted Molecular Mass
113 kDa

Product Datasheets

You must select a language.

x

E-594

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-594

Formulation Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: USP15

Ubiquitin carboxyl-terminal hydrolase 15 (USP15) is a specialized cysteine protease with a predicted molecular weight of 112 kDa.  USP15 is a member of the peptidase C19 family and the human protein shares 98% amino acid sequence identity with its mouse ortholog.  USP15 is a regulator of the TGF beta signaling pathway, where it may promote signaling strength by deubiquitination of SMAD proteins and/or the TGF beta R1.  Other reported targets of USP15 include histone H2B, NF kappa BI alpha, MDM2 and human papillomavirus type 16 E6 protein.

References
 
  1. Eichhorn P.J., et al. (2012) Nat. Med. 18: 429.
  2. Long L., et al. (2014) J. Biol. Chem. 289: 8916.
  3. Voss R.M., et al. (2009) J. Virol. 83: 8885.
  4. Zou Q., et al. (2014) Nat. Immunol. 15: 562.
 
Long Name
Ubiquitin Specific Protease 15
Entrez Gene IDs
9958 (Human); 14479 (Mouse); 171329 (Rat)
Alternate Names
Deubiquitinating enzyme 15; EC 3.1.2.15; EC 3.4.19.12; Gcap18; KIAA0529; KIAA0529ubiquitin carboxyl-terminal hydrolase 15; MGC131982; MGC149838; MGC74854; ubiquitin specific peptidase 15; ubiquitin specific protease 15; ubiquitin thioesterase 15; Ubiquitin thiolesterase 15; Ubiquitin-specific-processing protease 15; Ubp109; UNPH-2; UNPH4; USP15

Citations for Recombinant Human His6-USP15 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Accelerating inhibitor discovery for deubiquitinating enzymes
    Authors: WC Chan, X Liu, RS Magin, NM Girardi, SB Ficarro, W Hu, MI Tarazona G, CA Starnbach, A Felix, G Adelmant, AC Varca, B Hu, AS Bratt, E DaSilva, NJ Schauer, I Jaen Maiso, EK Dolen, AX Ayala, JA Marto, SJ Buhrlage
    Nature Communications, 2023-02-08;14(1):686.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  2. Site-specific ubiquitylation acts as a regulator of linker histone H1
    Authors: E Höllmüller, S Geigges, ML Niedermeie, KM Kammer, SM Kienle, D Rösner, M Scheffner, A Marx, F Stengel
    Nature Communications, 2021-06-09;12(1):3497.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. Structural and Functional Characterization of Ubiquitin Variant Inhibitors of USP15.
    Authors: Teyra J, Singer A, Schmitges F, Jaynes P, Kit Leng Lui S, Polyak M, Fodil N, Krieger J, Tong J, Schwerdtfeger C, Brasher B, Ceccarelli D, Moffat J, Sicheri F, Moran M, Gros P, Eichhorn P, Lenter M, Boehmelt G, Sidhu S
    Structure, 2019-01-31;0(0):.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human His6-USP15 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human His6-USP15 Protein, CF and earn rewards!

Have you used Recombinant Human His6-USP15 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review